Literature DB >> 33236225

Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling.

Duy Ba Ngo1, Thanyarat Chaibun2, Lee Su Yin3, Benchaporn Lertanantawong4, Werasak Surareungchai5,6.   

Abstract

The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 (HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry (SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 ± 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported.Graphical abstract.

Entities:  

Keywords:  Electrochemical DNA sensor; Functionalization; HEV3; PbS quantum dots

Mesh:

Substances:

Year:  2020        PMID: 33236225     DOI: 10.1007/s00216-020-03061-1

Source DB:  PubMed          Journal:  Anal Bioanal Chem        ISSN: 1618-2642            Impact factor:   4.142


  41 in total

1.  Efficacy and safety of a recombinant hepatitis E vaccine in healthy adults: a large-scale, randomised, double-blind placebo-controlled, phase 3 trial.

Authors:  Feng-Cai Zhu; Jun Zhang; Xue-Feng Zhang; Cheng Zhou; Zhong-Ze Wang; Shou-Jie Huang; Hua Wang; Chang-Lin Yang; Han-Min Jiang; Jia-Ping Cai; Yi-Jun Wang; Xing Ai; Yue-Mei Hu; Quan Tang; Xin Yao; Qiang Yan; Yang-Ling Xian; Ting Wu; Yi-Min Li; Ji Miao; Mun-Hon Ng; James Wai-Kuo Shih; Ning-Shao Xia
Journal:  Lancet       Date:  2010-08-20       Impact factor: 79.321

Review 2.  Development of new hepatitis E vaccines.

Authors:  Yufeng Cao; Zhenhong Bing; Shiyu Guan; Zecai Zhang; Xinping Wang
Journal:  Hum Vaccin Immunother       Date:  2018-06-18       Impact factor: 3.452

Review 3.  Hepatitis E virus infection.

Authors:  Nassim Kamar; Harry R Dalton; Florence Abravanel; Jacques Izopet
Journal:  Clin Microbiol Rev       Date:  2014-01       Impact factor: 26.132

4.  Distribution of human polyomaviruses, adenoviruses, and hepatitis E virus in the environment and in a drinking-water treatment plant.

Authors:  Nestor Albinana-Gimenez; Pilar Clemente-Casares; Silvia Bofill-Mas; Ayalkibet Hundesa; Ferran Ribas; Rosina Girones
Journal:  Environ Sci Technol       Date:  2006-12-01       Impact factor: 9.028

5.  Hidden danger: the raw facts about hepatitis E virus.

Authors:  Robert H Purcell; Suzanne U Emerson
Journal:  J Infect Dis       Date:  2010-09-15       Impact factor: 5.226

Review 6.  Hepatitis E: an emerging disease.

Authors:  María Teresa Pérez-Gracia; Beatriz Suay; María Luisa Mateos-Lindemann
Journal:  Infect Genet Evol       Date:  2014-01-13       Impact factor: 3.342

7.  Hepatitis E Virus: Animal Models and Zoonosis.

Authors:  Scott P Kenney; Xiang-Jin Meng
Journal:  Annu Rev Anim Biosci       Date:  2018-10-04       Impact factor: 8.923

Review 8.  Food and environmental routes of Hepatitis E virus transmission.

Authors:  Wim H M Van der Poel
Journal:  Curr Opin Virol       Date:  2014-02-08       Impact factor: 7.090

9.  Prevalence of hepatitis E virus antibodies and infection in New Zealand blood donors.

Authors:  Joanne Hewitt; David Harte; Michelle Sutherland; Dawn Croucher; Lindsey Fouche; Peter Flanagan; Deborah Williamson
Journal:  N Z Med J       Date:  2018-02-02

Review 10.  Zoonotic and foodborne transmission of hepatitis E virus.

Authors:  Xiang-Jin Meng
Journal:  Semin Liver Dis       Date:  2013-04-05       Impact factor: 6.115

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