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Literature DB >> 3323533

A 38 base-pair segment of DNA is required in cis for conjugative mobilization of broad host-range plasmid R1162.

Abstract

oriT, the region required in cis for conjugative mobilization of broad host-range plasmid R1162, has been localized to a 38 base-pair segment of DNA. The oriT DNA is also required for conjugation-dependent recombination. Point mutations at the HinPI cleavage site within oriT affect both mobilization and recombination, and the crossover location has been mapped to this site. An inverted repeat ten base-pairs from the recombination site is also involved in mobilization and recombination, and may be a recognition site for proteins involved in cleavage of the oriT DNA. The properties of conjugation-dependent recombination suggest that mobilization entails the formation of a linear intermediate that is transferred with both a unique origin and polarity.

Entities: Species

Mesh：

Substances：
DNA, Bacterial

Year: 1987 PMID： 3323533 DOI： 10.1016/0022-2836(87)90286-5

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

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Cited

31 in total

Review 1. Conjugative plasmid transfer in gram-positive bacteria.

Authors: Elisabeth Grohmann; Günther Muth; Manuel Espinosa
Journal: Microbiol Mol Biol Rev Date: 2003-06 Impact factor: 11.056

2. Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IncW plasmid R388 and comparison with the related IncN plasmid R46.

Authors: M Llosa; S Bolland; F de la Cruz
Journal: Mol Gen Genet Date: 1991-05

3. Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer.

Authors: Sarah Jandle; Richard Meyer
Journal: J Bacteriol Date: 2006-01 Impact factor: 3.490

4. MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA.

Authors: T Perwez; R Meyer
Journal: J Bacteriol Date: 1996-10 Impact factor: 3.490

5. Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the staphylococcal plasmid pGO1.

Authors: M W Climo; V K Sharma; G L Archer
Journal: J Bacteriol Date: 1996-08 Impact factor: 3.490

A 38 base-pair segment of DNA is required in cis for conjugative mobilization of broad host-range plasmid R1162.

Review 1. Conjugative plasmid transfer in gram-positive bacteria.

2. Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IncW plasmid R388 and comparison with the related IncN plasmid R46.

3. Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer.

4. MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA.

5. Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the staphylococcal plasmid pGO1.

6. Mutational analysis of the R64 oriT region: requirement for precise location of the NikA-binding sequence.

7. Localization of the nic site of IncN conjugative plasmid pCU1 through formation of a hybrid oriT.

8. Conjugal mobilization of streptococcal plasmid pMV158 between strains of Lactococcus lactis subsp. lactis.

9. Isolation and molecular characterization of pMG160, a mobilizable cryptic plasmid from Rhodobacter blasticus.

10. Conjugation-independent, site-specific recombination at the oriT of the IncW plasmid R388 mediated by TrwC.