A T Kabamba1, B T Kalunga2, C M Mwamba3, C M Nyembo3, F Dufrasne4, G Dessilly4, B M Kabamba4, A O Longanga2. 1. Laboratoire de biologie clinique, faculté des sciences pharmaceutiques, université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo; Pôle de microbiologie, institut de recherche expérimentale et clinique, université catholique de Louvain, Brussels, Belgium. Electronic address: arsene.kabamba@uclouvain.be. 2. Laboratoire de biologie clinique, faculté des sciences pharmaceutiques, université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo. 3. Faculté de médecine, université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo. 4. Pôle de microbiologie, institut de recherche expérimentale et clinique, université catholique de Louvain, Brussels, Belgium.
Abstract
OBJECTIVES: The strains of HBV circulating among blood donors in Lubumbashi, Democratic Republic of Congo (DRC), are not yet characterized. The purpose of this study was to determine seroprevalence, changes in biochemical parameters during HBV infection and molecular characterization of HBV in blood donors in Lubumbashi. METHODS: The detection of HBsAg was carried out by rapid diagnostic test then confirmed by the Liaison XL® Quant HBsAg technique. PCR targeting the P gene was carried out on LightCycler® 96 and genotyping by the sequencing technique on ABI 3500. RESULTS: The seroprevalence was 7.9%. The genotypes E (53.1%), A (41.8%), A3/E (3.8%), A1/E (1.3%) and some drug resistance mutations were identified. Disturbances of HDL-cholesterol, direct bilirubin, transaminases (ASAT and ALAT), PAL, GGT and albumin have been observed in HBsAg positive blood donors. CONCLUSION: The results of our study indicated that Lubumbashi is in a region with high endemicity for HBV and report for the first time HBV of genotypes A, E, A1/E and A3/E. They highlight the need to implement strategies to improve transfusion safety in blood transfusion centers and hospital blood banks in Lubumbashi in order to reduce HBV infection in recipients. They could also contribute to the implementation of treatment strategies and the development of mapping of circulating HBV genotypes in the DRC.
OBJECTIVES: The strains of HBV circulating among blood donors in Lubumbashi, Democratic Republic of Congo (DRC), are not yet characterized. The purpose of this study was to determine seroprevalence, changes in biochemical parameters during HBV infection and molecular characterization of HBV in blood donors in Lubumbashi. METHODS: The detection of HBsAg was carried out by rapid diagnostic test then confirmed by the Liaison XL® Quant HBsAg technique. PCR targeting the P gene was carried out on LightCycler® 96 and genotyping by the sequencing technique on ABI 3500. RESULTS: The seroprevalence was 7.9%. The genotypes E (53.1%), A (41.8%), A3/E (3.8%), A1/E (1.3%) and some drug resistance mutations were identified. Disturbances of HDL-cholesterol, direct bilirubin, transaminases (ASAT and ALAT), PAL, GGT and albumin have been observed in HBsAg positive blood donors. CONCLUSION: The results of our study indicated that Lubumbashi is in a region with high endemicity for HBV and report for the first time HBV of genotypes A, E, A1/E and A3/E. They highlight the need to implement strategies to improve transfusion safety in blood transfusion centers and hospital blood banks in Lubumbashi in order to reduce HBV infection in recipients. They could also contribute to the implementation of treatment strategies and the development of mapping of circulating HBV genotypes in the DRC.
Authors: Armel M Sanou; Achille S Nikièma; Seimbou Zalla; Mamadou Ouattara; Nina Pascaline S Dakouo; Alice Kiba-Koumare; Mariam Seynou; Delphine Napon-Zongo; Roger Sombié Journal: Health Sci Rep Date: 2022-08-08