Gisela Hobson Pontes1, Fernando Sérgio Mendes Carneiro Filho, Luis Alejandro Vargas Guerrero2, Leandro Cavalcante Lipinski3, Lucia de Noronha4, Eduardo Nascimento Silva5, Fernando Serra-Guimarães6. 1. Postgraduate Program in Physiopathology and Surgical Sciences, State University of Rio de Janeiro (UERJ), Rio de Janeiro, RJ, Brazil. 2. Resident in the Plastic Surgery Training Program at Ronaldo Pontes Plastic Surgery, Bogota-CUN, Colombia. 3. Postgraduate Program in Health Sciences, State University of Ponta Grossa (UEPG), Ponta Grossa-PR, Brazil. 4. Anatomical Pathology, Pontifical Catholic University of Paraná (PUC-PR), Curitiba-PR, Brazil. 5. Plastic Surgery and Anatomy, State University of Ponta Grossa (UEPG), Ponta Grossa-PR, Brazil. 6. Postgraduate Program in Physiopathology and Surgical Sciences, State University of Rio de Janeiro (UERJ).
Abstract
BACKGROUND: In the biological response to biomaterials, the implant shell plays a key role in immune and inflammatory reactions. We hypothesized that the capsules formed around nanotextured implants exhibit an immunohistochemical behavior different to those formed around polyurethane implants. OBJECTIVES: The aim of this study was to evaluate through immunohistochemistry markers the capsules formed around nanotextured and polyurethane implants. METHODS: Sixty albino female Wistar rats were divided into 2 groups (nanotextured and polyurethane), with 30 animals in each group. A mini silicone implant was inserted on the back of the animals. After a predetermined period, the animals were killed, and the capsules formed around the implants were studied. The capsules in the 30-, 60-, and 90-day subgroups were analyzed via immunohistochemistry to detect markers for fibroblast α smooth muscle actin (α-SMA), transforming growth factor β (TGF-β), cluster of differentiation 34 (CD34), and CD68, via picrosirius staining to determine the density of type I and III collagen fibers and via hematoxylin and eosin staining to assess capsule thickness. A Wilcoxon-Mann-Whitney test was used to compare the groups, and a Kruskal-Wallis test was used to compare the subgroups. RESULTS: Lower α-SMA, TGF-β, CD34 and CD68 immunoexpression was observed in the nanotextured 30- and 60-day subgroups than in the corresponding polyurethane subgroups. In the 90-day subgroup, more pronounced α-SMA and CD34 immunoexpression was observed in the nanotextured group; however, TGF-β and CD68 immunoexpression remained lower. The nanotextured implants showed reduced capsular thickness and greater formation of type I collagen in all the analyzed subgroups. CONCLUSIONS: Nanotextured implants led to reduced immune and inflammatory reactions compared with polyurethane implants according to all analyzed variables.
BACKGROUND: In the biological response to biomaterials, the implant shell plays a key role in immune and inflammatory reactions. We hypothesized that the capsules formed around nanotextured implants exhibit an immunohistochemical behavior different to those formed around polyurethane implants. OBJECTIVES: The aim of this study was to evaluate through immunohistochemistry markers the capsules formed around nanotextured and polyurethane implants. METHODS: Sixty albino female Wistar rats were divided into 2 groups (nanotextured and polyurethane), with 30 animals in each group. A mini silicone implant was inserted on the back of the animals. After a predetermined period, the animals were killed, and the capsules formed around the implants were studied. The capsules in the 30-, 60-, and 90-day subgroups were analyzed via immunohistochemistry to detect markers for fibroblast α smooth muscle actin (α-SMA), transforming growth factor β (TGF-β), cluster of differentiation 34 (CD34), and CD68, via picrosirius staining to determine the density of type I and III collagen fibers and via hematoxylin and eosin staining to assess capsule thickness. A Wilcoxon-Mann-Whitney test was used to compare the groups, and a Kruskal-Wallis test was used to compare the subgroups. RESULTS: Lower α-SMA, TGF-β, CD34 and CD68 immunoexpression was observed in the nanotextured 30- and 60-day subgroups than in the corresponding polyurethane subgroups. In the 90-day subgroup, more pronounced α-SMA and CD34 immunoexpression was observed in the nanotextured group; however, TGF-β and CD68 immunoexpression remained lower. The nanotextured implants showed reduced capsular thickness and greater formation of type I collagen in all the analyzed subgroups. CONCLUSIONS: Nanotextured implants led to reduced immune and inflammatory reactions compared with polyurethane implants according to all analyzed variables.
Authors: Rodrigo Bredariol Achilles; Daniel Vitor DE Souza; Hananiah Tardivo Quintana; Andrea Cristina Moraes Malinverni; Carolina Foot Gomes DE Moura; Giovana Wagner Branda; Ana Claudia Muniz Renno; Daniel Araki Ribeiro Journal: In Vivo Date: 2022 May-Jun Impact factor: 2.406
Authors: Ralf Berger; Jurandir Marcondes Ribas Filho; Marcelo Augusto de Souza; Pedro Henrique de Paula; João Gabriel Cavazzani Doubek; Rafael de Castro E Souza Pires; Paulo Afonso Nunes Nassif; Eduardo Nascimento Silva Journal: Acta Cir Bras Date: 2022-04-22 Impact factor: 1.564