Chang Shu1,2, Andrew E Jaffe1,3,4, Sarven Sabunciyan5, Hongkai Ji4, Jacquie Astemborski6, Jing Sun6, Kelly M Bakulski7, Shruti H Mehta6, Gregory D Kirk6, Brion S Maher1. 1. Department of Mental Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA. 2. Department of Pediatrics, Columbia University Irving Medical Center, New York, NY 10032, USA. 3. Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA. 4. Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA. 5. Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. 6. Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA. 7. Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA.
Abstract
Aim: To investigate the role of epigenetics in HIV pathophysiology. Materials & methods: We conducted an epigenome-wide association scan on HIV infection status among people who inject drugs in the AIDS Linked to the IntraVenous Experience study with primary (n = 397) and validation samples (n = 390). DNA methylation from blood was measured by the Illumina EPIC BeadChip. We controlled for cell type heterogeneity by HIV status. Results: HIV infection status was associated (p < 10-8) with DNA methylation at 49 CpG sites. Sites were enriched in response to virus, interferon signaling pathway, etc. Among these sites, discovery and validation t-statistics were highly correlated (r = 0.96). Conclusion: In a cohort of people who inject drugs, HIV status was associated with differential DNA methylation at biologically meaningful sites.
Aim: To investigate the role of epigenetics in HIV pathophysiology. Materials & methods: We conducted an epigenome-wide association scan on HIV infection status among people who inject drugs in the AIDS Linked to the IntraVenous Experience study with primary (n = 397) and validation samples (n = 390). DNA methylation from blood was measured by the Illumina EPIC BeadChip. We controlled for cell type heterogeneity by HIV status. Results:HIV infection status was associated (p < 10-8) with DNA methylation at 49 CpG sites. Sites were enriched in response to virus, interferon signaling pathway, etc. Among these sites, discovery and validation t-statistics were highly correlated (r = 0.96). Conclusion: In a cohort of people who inject drugs, HIV status was associated with differential DNA methylation at biologically meaningful sites.
Entities:
Keywords:
DNA methylation; HIV; epigenetics; epigenome-wide association; people who inject drugs
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