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Literature DB >> 33226183

Comparison of separation modes for microchip electrophoresis of proteins.

Thushara N Samarasinghe¹, Yong Zeng¹, Carey K Johnson¹.

Abstract

Separation of a set of model proteins was tested on a microchip electrophoresis analytical platform capable of sample injection by two different electrokinetic mechanisms. A range of separation modes-microchip capillary zone electrophoresis, microchip micellar electrokinetic chromatography, and nanoparticle-based sieving-was tested on glass and polydimethylsiloxane/glass microchips and with silica-nanoparticle colloidal arrays. The model proteins calmodulin (18 kiloDalton), bovine serum albumin (66 kDa), and concanavalin (106 kDa) were labeled with Alexa Fluor 647 for laser-induced fluorescence detection. The best separation and resolution were obtained in a silica-nanoparticle colloidal array chip.

Entities: Chemical

Keywords: capillary electrophoresis; electrokinetic injection; laser induced fluorescence detection; microchip electrophoresis

Mesh：

Substances：

Year: 2020 PMID： 33226183 PMCID： PMC8053037 DOI： 10.1002/jssc.202000883

Source DB: PubMed Journal: J Sep Sci ISSN： 1615-9306 Impact factor: 3.645

42 in total

Review 1. Capillary electrophoresis of proteins 2003-2005.

Authors: Vladislav Dolník
Journal: Electrophoresis Date: 2006-01 Impact factor: 3.535

Review 2. Separation media for microchips.

Authors: Mary J Wirth
Journal: Anal Chem Date: 2007-02-01 Impact factor: 6.986

3. Self-assembled colloidal arrays as three-dimensional nanofluidic sieves for separation of biomolecules on microchips.

Authors: Yong Zeng; D Jed Harrison
Journal: Anal Chem Date: 2007-02-16 Impact factor: 6.986

Review 4. Recent innovations in protein separation on microchips by electrophoretic methods: an update.

Authors: Nguyet Thuy Tran; Ichraf Ayed; Antoine Pallandre; Myriam Taverna
Journal: Electrophoresis Date: 2010-01 Impact factor: 3.535

5. Unified theory for gel electrophoresis and gel filtration.

Authors: D Rodbard; A Chrambach
Journal: Proc Natl Acad Sci U S A Date: 1970-04 Impact factor: 11.205

6. Molecular-sieve chromatography and electrophoresis in polyacrylamide gels.

Authors: C J Morris; P Morris
Journal: Biochem J Date: 1971-09 Impact factor: 3.857

7. Microdialysis sampling coupled to microchip electrophoresis with integrated amperometric detection on an all-glass substrate.

Authors: David E Scott; Ryan J Grigsby; Susan M Lunte
Journal: Chemphyschem Date: 2013-06-21 Impact factor: 3.102

8. Droplet sample introduction to microchip gel and zone electrophoresis for rapid analysis of protein-protein complexes and enzymatic reactions.

Authors: Claire M Ouimet; Cara I D'Amico; Robert T Kennedy
Journal: Anal Bioanal Chem Date: 2019-07-13 Impact factor: 4.142

9. Sodium dodecyl sulfate-capillary gel electrophoresis of proteins using non-cross-linked polyacrylamide.

Authors: D Wu; F E Regnier
Journal: J Chromatogr Date: 1992-09-11

10. Hepatic and serologic toxicity of systemic interleukin-2 and/or interferon-alpha. Evidence of a risk-benefit advantage of subcutaneous therapy.

Authors: A Schomburg; H Kirchner; E Lopez-Hänninen; T Menzel; P Rudolph; A Körfer; M Fenner; H Poliwoda; J Atzpodien
Journal: Am J Clin Oncol Date: 1994-06 Impact factor: 2.339

1 in total

1. Microchip Electrophoresis Assay for Calmodulin Binding Proteins.

Authors: Thushara N Samarasinghe; Yong Zeng; Carey K Johnson
Journal: J Sep Sci Date: 2020-12-07 Impact factor: 3.645

1 in total