| Literature DB >> 3322286 |
J W Kwak1, J Kim, O J Yoo, M H Han.
Abstract
A method convenient for isolation of DNA sequences capable of directing gene transcription in both organisms of E. coli and yeast is described. The method is composed of sequential steps of phenotypic selection for chloramphenicol resistance, first in E. coli and then in yeast. A series of promoter-probe, shuttle plasmid vectors between yeast and E. coli were constructed and utilized in the method.Entities:
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Year: 1987 PMID： 3322286 DOI： 10.1016/0006-291x(87)90485-2
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575