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Literature DB >> 3322263

The role of the metal ion in the mechanism of the K+-activated aldehyde dehydrogenase of Saccharomyces cerevisiae.

Abstract

The effect of K+ on assays of the enzyme was studied and it appears that the activation occurs slowly by a two-step process. Kinetic measurements suggest that the enzyme-catalysed reaction can proceed slowly (0.4%) in the complete absence of K+. The enzyme exhibits a K+-activated esterase activity, which is further activated by NAD+ or NADH. Stopped-flow studies indicated that the principal effect of K+ on the dehydrogenase reaction is to accelerate a step (possibly acyl-enzyme hydrolysis) associated with a fluorescence and small absorbance transient that occurs after hydride transfer and before NADH dissociation from the terminal E-NADH complex. The variation of activity of the enzyme with pH was studied. An enzyme group with pKa approx. 7.1 apparently promotes enzyme activity when in its alkaline form.

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Year: 1987 PMID： 3322263 PMCID： PMC1148419 DOI： 10.1042/bj2470377

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

20 in total

1. Kinetics of the glyceraldehyde 3-phosphate dehydrogenase-catalyzed hydrolysis of p-nitrophenyl acetate.

Authors: M T Behme; E H Cordes
Journal: J Biol Chem Date: 1967-12-10 Impact factor: 5.157

2. Studies on the mechanism of sheep liver cytosolic aldehyde dehydrogenase. The effect of pH on the aldehyde binding reactions and a re-examination of the problem of the site of proton release in the mechanism.

Authors: F M Dickinson
Journal: Biochem J Date: 1986-08-15 Impact factor: 3.857

3. Rapid purification and properties of potassium-activated aldehyde dehydrogenase from Saccharomyces cerevisiae.

Authors: K A Bostian; G F Betts
Journal: Biochem J Date: 1978-09-01 Impact factor: 3.857

4. Transient kinetics of nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria.

Authors: K Dalziel; N McFerran; B Matthews; C H Reynolds
Journal: Biochem J Date: 1978-06-01 Impact factor: 3.857

9. The effects of Mg2+ on certain steps in the mechanisms of the dehydrogenase and esterase reactions catalysed by sheep liver aldehyde dehydrogenase. Support for the view that dehydrogenase and esterase activities occur at the same site on the enzyme.

Authors: F M Dickinson; G W Haywood
Journal: Biochem J Date: 1986-02-01 Impact factor: 3.857

10. Effects of Mg2+, Ca2+ and Mn2+ on sheep liver cytoplasmic aldehyde dehydrogenase.

Authors: F M Dickinson; G J Hart
Journal: Biochem J Date: 1982-08-01 Impact factor: 3.857

3 in total

1. Studies on the unusual behaviour of bovine liver UDP-glucose dehydrogenase in assays at acid and neutral pH and on the presence of tightly bound nucleotide material in purified preparations of this enzyme.

Authors: F M Dickinson
Journal: Biochem J Date: 1988-11-01 Impact factor: 3.857

2. Purification and characterization of benzaldehyde dehydrogenase I from Acinetobacter calcoaceticus.

Authors: R M Chalmers; C A Fewson
Journal: Biochem J Date: 1989-11-01 Impact factor: 3.857

3. The purification and some properties of the Mg(2+)-activated cytosolic aldehyde dehydrogenase of Saccharomyces cerevisiae.

Authors: F M Dickinson
Journal: Biochem J Date: 1996-04-15 Impact factor: 3.857