Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells.

Literature DB >> 33222139

Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells.

Sébastien Houy¹, Joana S Martins¹, Ralf Mohrmann², Jakob Balslev Sørensen³.

Abstract

Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.

Entities: Chemical Species

Keywords: Calcium measurements; Capacitance measurement; Carbon-fiber amperometry; Chromaffin cells; Electrophysiology; Exocytosis; Neurosecretion; Patch-clamp; Vesicles

Mesh：

Substances：
Calcium

Year: 2021 PMID： 33222139 DOI： 10.1007/978-1-0716-1044-2_16

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

45 in total

Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells.

Review 1. Exocytosis: the chromaffin cell as a model system.

Review 2. Emerging roles of presynaptic proteins in Ca++-triggered exocytosis.

Review 3. Formation, stabilisation and fusion of the readily releasable pool of secretory vesicles.

Review 4. Vesicle docking in regulated exocytosis.

5. Transport, docking and exocytosis of single secretory granules in live chromaffin cells.

Review 6. How does the stimulus define exocytosis in adrenal chromaffin cells?

7. Differential control of the releasable vesicle pools by SNAP-25 splice variants and SNAP-23.

8. Vesicle pools: lessons from adrenal chromaffin cells.

Review 9. Neurosecretion: what can we learn from chromaffin cells.

Review 10. v-SNARE function in chromaffin cells.

1. Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca²⁺-dependent priming.

2. Membrane Capacitance Measurements of Stimulus-Evoked Exocytosis in Adrenal Chromaffin Cells.

3. Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion.

4. A Novel Role of the TRPM4 Ion Channel in Exocytosis.

Review 5. Vesicle Fusion as a Target Process for the Action of Sphingosine and Its Derived Drugs.