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Literature DB >> 3322193

Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.

P K Jepsen¹, E Riise, K Biedermann, P C Kristensen, C Emborg.

Abstract

Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.

Entities: Species

Mesh：

Substances：
Deoxyribonucleases

Year: 1987 PMID： 3322193 PMCID： PMC204152 DOI： 10.1128/aem.53.10.2593-2596.1987

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

11 in total

1. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.

Authors: J Collins; B Hohn
Journal: Proc Natl Acad Sci U S A Date: 1978-09 Impact factor: 11.205

2. [Effect of the composition of the culture medium on the growth and deoxyribonuclease activity of pigmented and nonpigmented strains of Serratia marcescens].

Authors: D V Iusupova; A Z Gareishina; M I Beliaeva
Journal: Prikl Biokhim Mikrobiol Date: 1972 Nov-Dec

3. Pleiotropic mutations in Serratia marcescens which increase the synthesis of certain exocellular proteins and the rate of spontaneous prophage induction.

Authors: U Winkler; K Timmis
Journal: Mol Gen Genet Date: 1973-08-17

4. Gene dosage studies with pleiotropic mutants of Serratia marcescens superactive in the synthesis of marcescin A and certain other exocellular proteins.

Authors: K Timmis; U Winkler
Journal: Mol Gen Genet Date: 1973-08-17

Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.

1. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.

2. [Effect of the composition of the culture medium on the growth and deoxyribonuclease activity of pigmented and nonpigmented strains of Serratia marcescens].

3. Pleiotropic mutations in Serratia marcescens which increase the synthesis of certain exocellular proteins and the rate of spontaneous prophage induction.

4. Gene dosage studies with pleiotropic mutants of Serratia marcescens superactive in the synthesis of marcescin A and certain other exocellular proteins.

5. An extracellular nuclease from Serratia marcescens. I. Purification and some properties of the enzyme.

6. Secretion of staphylococcal nuclease by Bacillus subtilis.

7. EFFECT OF PH ON THE FORMATION OF EXOCELLULAR NUCLEASE IN AGING BROTH CULTURES OF SERRATIA MARCESCENS.

8. Production of extracellular and total invertase by Candida utilis, Saccharomyces cerevisiae, and other yeasts.

9. Isolation and characterization of nucleases from a clinical isolate of Serratia marcescens kums 3958.

10. Isolation of a Serratia marcescens mutant which is an efficient recipient for the E. coli episome.

1. Influence of growth temperature and lipopolysaccharide on hemolytic activity of Serratia marcescens.

2. Fermentation studies of the secretion of Serratia marcescens nuclease by Escherichia coli.

3. Influence of temperature on the physiology and virulence of the insect pathogen Serratia sp. Strain SCBI.