Rocío Morón1,2, Alba Rodríguez-Nogales3,1,4, Francesca Algieri3, José Garrido-Mesa3, Teresa Vezza5,6, María Jesús Rodríguez-Sojo3,1, María Elena Rodríguez-Cabezas3,1, Mónica Olivares7, Federico García1,8, Julio Gálvez3,1. 1. Instituto de Investigación Biosanitaria de Granada (Ibs.GRANADA), 18012, Granada, Spain. 2. Servicio Farmacia Hospitalaria, Hospital Universitario Clínico San Cecilio, 18100, Granada, Spain. 3. CIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071, Granada, Spain. 4. Servicio de Digestivo, Hospital Universitario Virgen de las Nieves, 18012, Granada, Spain. 5. CIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071, Granada, Spain. teresavezza@hotmail.it. 6. Instituto de Investigación Biosanitaria de Granada (Ibs.GRANADA), 18012, Granada, Spain. teresavezza@hotmail.it. 7. Research Department of Biosearch, 18004, Granada, Spain. 8. Servicio Microbiología, Hospital Universitario Clínico San Cecilio, 18100, Granada, Spain.
Abstract
PURPOSE: Probiotics have been shown to exert beneficial effects in IBD although their exact mechanisms are not completely understood. The aim of the present study was to assess the intestinal anti-inflammatory activity of different probiotics (Lactobacillus fermentum CECT5716, Lactobacillus salivarius CECT5713, Escherichia coli Nissle 1917, Saccharomyces boulardii CNCMI-745 in the dinitrobenzene sulfonic acid (DNBS) model of mouse colitis and correlate it with the modifications of the gut microbiota and the immune response, focusing on miRNA expression. METHODS: The probiotics were daily administered orally for 25 days. On day 19 colitis was induced by rectal installation of DNBS. At the end of the treatment, mice were sacrificed and the colonic damage was assessed biochemically by analysing the expression of different markers involved in the immune response, including miRNAs; and the colonic microbiota by pyrosequencing. Probiotics properties were also evaluated in vitro in different immune cell types (CMT-93 intestinal epithelial cells and bone marrow-derived macrophages), where the expression of different mRNAs and miRNAs was examined. RESULTS: All the probiotics displayed intestinal anti-inflammatory effects but slightly different, especially regarding miRNAs expression. Likewise, the probiotics ameliorated the colitis-associated dysbiosis, although showing differences in the main bacterial groups affected. CONCLUSION: Among the probiotics assayed, Lactobacillus fermentum CECT5716 and Escherichia coli Nissle 1917 appear to present the best intestinal anti-inflammatory effects, being the latter one of the few probiotics with reputed efficacy in human IBD. Therefore, Lactobacillus fermentum CECT5716 could be considered as a complementary nutritional strategy for IBD treatment.
PURPOSE: Probiotics have been shown to exert beneficial effects in IBD although their exact mechanisms are not completely understood. The aim of the present study was to assess the intestinal anti-inflammatory activity of different probiotics (Lactobacillus fermentum CECT5716, Lactobacillus salivarius CECT5713, Escherichia coli Nissle 1917, Saccharomyces boulardii CNCMI-745 in the dinitrobenzene sulfonic acid (DNBS) model of mouse colitis and correlate it with the modifications of the gut microbiota and the immune response, focusing on miRNA expression. METHODS: The probiotics were daily administered orally for 25 days. On day 19 colitis was induced by rectal installation of DNBS. At the end of the treatment, mice were sacrificed and the colonic damage was assessed biochemically by analysing the expression of different markers involved in the immune response, including miRNAs; and the colonic microbiota by pyrosequencing. Probiotics properties were also evaluated in vitro in different immune cell types (CMT-93 intestinal epithelial cells and bone marrow-derived macrophages), where the expression of different mRNAs and miRNAs was examined. RESULTS: All the probiotics displayed intestinal anti-inflammatory effects but slightly different, especially regarding miRNAs expression. Likewise, the probiotics ameliorated the colitis-associated dysbiosis, although showing differences in the main bacterial groups affected. CONCLUSION: Among the probiotics assayed, Lactobacillus fermentum CECT5716 and Escherichia coli Nissle 1917 appear to present the best intestinal anti-inflammatory effects, being the latter one of the few probiotics with reputed efficacy in human IBD. Therefore, Lactobacillus fermentum CECT5716 could be considered as a complementary nutritional strategy for IBD treatment.
Authors: Alba Rodríguez-Nogales; Francesca Algieri; José Garrido-Mesa; Teresa Vezza; M Pilar Utrilla; Natalia Chueca; Federico García; M Elena Rodríguez-Cabezas; Julio Gálvez Journal: J Nutr Biochem Date: 2018-09-01 Impact factor: 6.048
Authors: Alba Rodríguez-Nogales; Francesca Algieri; Jose Garrido-Mesa; Teresa Vezza; M Pilar Utrilla; Natalia Chueca; Federico Garcia; Mónica Olivares; M Elena Rodríguez-Cabezas; Julio Gálvez Journal: Mol Nutr Food Res Date: 2017-08-29 Impact factor: 5.914
Authors: Daniel N Frank; Allison L St Amand; Robert A Feldman; Edgar C Boedeker; Noam Harpaz; Norman R Pace Journal: Proc Natl Acad Sci U S A Date: 2007-08-15 Impact factor: 11.205
Authors: Alba Rodríguez-Nogales; Francesca Algieri; José Garrido-Mesa; Teresa Vezza; Maria P Utrilla; Natalia Chueca; Jose A Fernández-Caballero; Federico García; Maria E Rodríguez-Cabezas; Julio Gálvez Journal: Front Pharmacol Date: 2018-05-11 Impact factor: 5.810
Authors: Abd El-Nasser Khattab; Ahmed M Darwish; Sarah I Othman; Ahmed A Allam; Haifa A Alqhtani Journal: Biol Trace Elem Res Date: 2022-02-21 Impact factor: 3.738
Authors: María Jesús Rodríguez-Sojo; Antonio Jesús Ruiz-Malagón; María Elena Rodríguez-Cabezas; Julio Gálvez; Alba Rodríguez-Nogales Journal: Nutrients Date: 2021-03-21 Impact factor: 5.717