Claudinéia Pereira Maranduba1, Gustavo Torres Souza1, Antônio Márcio Resende do Carmo2, José Marcelo Sallabert de Campos3, Nádia Rezende Barbosa Raposo4, Marcelo de Olivera Santos5, Carlos Magno da Costa Maranduba6, Fernando de Sá Silva5. 1. Laboratory of Human Genetics and Cell Therapy, Biology Department, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, 36036-900, Brazil. 2. Department of Endodontology, Dentistry School, Federal University of Juiz de Fora, Juiz de Fora, 36036-900, Brazil. 3. Laboratory of Genetics, Biology Department, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, 36036-900, Brazil. 4. Center of Research and Innovation in Health Sciences (NUPICS), Federal University of Juiz de Fora, Juiz de Fora, 36036-900, Brazil. 5. Basic Life Sciences Department, Federal University of Juiz de Fora, Governador Valadares, 35020-670, Brazil. 6. Laboratory of Genetics, Biology Department, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, 36036-900, Brazil. carlos.maranduba@ufjf.edu.br.
Abstract
PURPOSE: We aimed at verifying whether resveratrol can decrease cell proliferation and change osteogenic differentiation of cells obtained from patients with type 1 neurofibromatosis (NF1). METHODS: Deciduous dental pulp derived stem cells were isolated from NF1 patient and healthy volunteer. These cells were subjected to increasing concentrations of resveratrol and evaluated for proliferation and mineralization of osteogenic differentiation. RESULTS: The results showed that resveratrol reduced the difference in proliferation between CNT and NF1 cells in a dose-dependent manner and this property was more prominent in affected cells than in healthy cells. Resveratrol showed no statistically significant changes in mineralization in osteogenic differentiation of NF1 cells, at low doses tested. CONCLUSIONS: In conclusion, in a dose-dependent manner, resveratrol displays interesting properties that could be applied in a possible treatment aimed at decreasing cellular proliferation in neurofibromatosis. Furthermore, it is selective concerning healthy cells and not affecting cell differentiation. Further research to cell selectivity, differentiation to other tissue types, and cell cytotoxicity are needed.
PURPOSE: We aimed at verifying whether resveratrol can decrease cell proliferation and change osteogenic differentiation of cells obtained from patients with type 1 neurofibromatosis (NF1). METHODS: Deciduous dental pulp derived stem cells were isolated from NF1patient and healthy volunteer. These cells were subjected to increasing concentrations of resveratrol and evaluated for proliferation and mineralization of osteogenic differentiation. RESULTS: The results showed that resveratrol reduced the difference in proliferation between CNT and NF1 cells in a dose-dependent manner and this property was more prominent in affected cells than in healthy cells. Resveratrol showed no statistically significant changes in mineralization in osteogenic differentiation of NF1 cells, at low doses tested. CONCLUSIONS: In conclusion, in a dose-dependent manner, resveratrol displays interesting properties that could be applied in a possible treatment aimed at decreasing cellular proliferation in neurofibromatosis. Furthermore, it is selective concerning healthy cells and not affecting cell differentiation. Further research to cell selectivity, differentiation to other tissue types, and cell cytotoxicity are needed.
Authors: Mohammad Athar; Jung Ho Back; Xiuwei Tang; Kwang Ho Kim; Levy Kopelovich; David R Bickers; Arianna L Kim Journal: Toxicol Appl Pharmacol Date: 2007-01-03 Impact factor: 4.219
Authors: Paula Nascimento Almeida; Gustavo Torres Souza; Camila Maurmann de Souza; Rafaella Souza Salomão de Zanette; Claudinéia Pereira Maranduba; João Vitor Paes Rettore; Marcelo Oliveira de Santos; Antônio Márcio Resende do Carmo; Carlos Magno Costa da Maranduba; Fernando Sá de Silva Journal: Childs Nerv Syst Date: 2014-12-06 Impact factor: 1.475