Differentiating synchronous double primary lung adenocarcinomas from intrapulmonary metastasis by CT features, EGFR mutations and ALK rearrangement status.

BACKGROUND: Differentiating synchronous double primary lung adenocarcinoma (SDPLA) from interpulmonary metastasis (IPM) has significant therapeutic and prognostic implications. This retrospective study aimed to investigate the potential of computed tomography (CT) features and two known oncogenic driver mutations [epidermal growth factor receptor (EGFR) and anaplastic large-cell lymphoma kinase (ALK)] to discriminate synchronous double primary lung adenocarcinoma from one primary pulmonary adenocarcinoma with intrapulmonary metastasis.
METHODS: Patients with SDPLA were selected at our hospital, and those with IPM served as the control group. All 60 patients (40 with SDPLA and 20 with IPM) were tested for EGFR mutations and ALK status, and they underwent chest CT prior to any treatment. Independent-sample Student's t-test was used for comparisons between two groups of normally distributed variables, and the Chi-square test was used to compare categorical variables.
RESULTS: The discordance rate of EGFR mutations was significantly higher in patients with SDPLA than in patients with IPM (40% vs. 5%, P<0.001). The incidence of ALK-positive status was 15%, and patients with IPM were more likely to be ALK-positive than patients with SDPLA (35% vs. 5%, P<0.001). Compared to IPM, SDPLA more frequently occurred in different lobes (P=0.024), presented with less lymphadenopathy (P=0.014), showed a smaller difference in diameter (Äd) between tumors (P=0.001) and more commonly presented as lobulated tumors (P<0.001), spiculated masses (P<0.001), ground-glass opacities (GGOs) (P=0.001) and air bronchograms (P=0.020).
CONCLUSION: Patients with SDPLA showed higher discordance with EGFR mutations and were less frequently ALK-positive than those with IPM. Thus, the CT characteristics are significantly different between SDPLA and IPM. 2020 Journal of Thoracic Disease. All rights reserved.

Introduction

In recent decades, lung cancer has become one of the leading causes of cancer-related deaths worldwide. The incidence of multiple primary lung cancers (MPLCs) in reported clinical series ranges from 1% to 7% (1). Recently, autopsy studies have more accurately revealed that the incidence of multiple primary tumors in lungs ranges from 3.5% to 14% (2). Differentiating synchronous double primary lung adenocarcinoma (SDPLA) from interpulmonary metastasis (IPM) has significant therapeutic and prognostic implications. However, when two tumors sharing common histological features exist simultaneously in the lungs, their classification as metastases or as separate primary tumors becomes challenging. Therefore, exploring more convenient and accurate methods to differentiate two concurrent lung tumors is important.

Criteria for the identification of multiple primary lung tumors were initially published by Martini and Melamed in 1975 (3) and were updated by the American College of Chest Physicians (ACCP) (4). These criteria are mainly based on tumor locations and histological findings, and neither set of criteria incorporates the use of molecular data to distinguish separate primary tumors from metastases. However, some cases do not completely meet these criteria (5). In recent years, studies have tried to identify SDPLA and IPM using molecular genetics and imaging techniques (2,6-9). Many indicators based on molecular genetics exist, such as epidermal growth factor receptor (EGFR)/KRAS mutation status (2,8), p53 mutation (6,10) and array comparative genomic hybridization (aCGH) (9). However, none of these methods have been widely recognized. Recent oncology and pathology practice guidelines recommend testing all locally advanced and metastatic adenocarcinomas for the most commonly targeted genetic test abnormalities, such as EGFR mutations and anaplastic lymphoma kinase (ALK) gene rearrangements. EGFR mutations (sensitizing activating mutations) are associated with tumor sensitivity to EGFR tyrosine kinase inhibitors (gefitinib, erlotinib and afatinib) (11), and crizotinib was the first drug approved for adenocarcinoma harboring ALK rearrangements (12,13). Therefore, EGFR and ALK tests may be helpful for distinguishing patients with SDPLA from those with IPM in a highly cost-effective manner.

Currently, computed tomography (CT) is the best method to detect and characterize pulmonary tumors (14). Some studies have supported the lack of characteristic imaging findings for SDPLA (10,15). However, signs of malignancy (e.g., lobulation, spiculate and pleural indentations) are often found in each MPLC tumor (16), and IPM lesions typically manifest as solid nodules with smooth margins on CT. Therefore, CT may possess the potential to discriminate SDPLA from IPM.

Therefore, we aimed to investigate the potential of two known oncogenic driver mutations (EGFR and ALK) and CT features to discriminate SDPLA from IPM disease. We also identified relationships among EGFR mutation status, clinical features and CT characteristics in patients with SDPLA.

We present the following article in accordance with the STROBE reporting checklist (available at http://dx.doi.org/10.21037/jtd-19-3570).

Methods

Patients and inclusion criteria

In total, 1,193 patients evaluated by the multidisciplinary thoracic oncology group between January 2012 and April 2018 at our institution were retrospectively screened. Among them, 40 patients (80 lesions) with two primary tumors, including a synchronous pulmonary tumor diagnosed within 6 months after diagnosis of the index tumor and lung cancer that metastasized to the same lobe (stage IIIb) or different lobes (stage IV), were consecutively screened and included in the control group (or IPM group) until the sample size was achieved (20 patients and 40 lesions). The inclusion criteria were as follows: (I) available pathology reports (including predominant pathological subtypes, lymph node metastasis and pleural invasion) indicating a diagnosis of lung adenocarcinoma; (II) available results for both EGFR mutations and ALK status; and (III) available clinical data, including age, sex, smoking history and tumor node metastasis (TNM) stage. The exclusion criteria were as follows: (I) thin-section CT was not available; (II) preoperative treatment was administered, such as radiation therapy or chemotherapy; and (III) surgery was not performed. An overview of the inclusion and exclusion criteria is presented in a flow chart ().

Figure 1

An overview of the inclusion and exclusion criteria (A) as well as molecular testing results (B). SDPLA, synchronous double primary lung adenocarcinoma; IPM, intrapulmonary metastasis; EGFR, epidermal growth factor receptor; ALK, anaplastic large-cell lymphoma kinase.

The criteria for the definitions of SDPLA and IPM were according to comprehensive histologic assessment (CHA) (17,18). SDPLA was histologically indicated as follows: (I) at least one of the multiple lesions was adenocarcinoma in situ (AIS); (II) the major histopathologic subtype was different among multiple lesions; (III) the major histopathologic subtype was similar, but there were differences in other histological subtypes/cytology/stroma; and (IV) the tumor-free interval between cancers was <2 years. In addition, IPM was indicated as follows: (I) similar major histopathologic subtype and similar other histological subtypes; and (II) similar major histopathologic subtype and similar growth pattern/architecture.

Patient clinical characteristics, including age, sex, smoking history, histopathology, tumor size, nodal involvement, distant metastasis, and tumor stage, were recorded. Non-smoking status was defined as lifetime exposure to fewer than 100 cigarettes, and the remaining patients were categorized as ever-smokers. Tumor node metastasis (TNM) staging was based on the IASLC TNM Lung Cancer Staging System (8th edition). All CT data were independently reviewed by two experienced radiologists who were blinded to EGFR and ALK status. The medical records of each patient were reviewed retrospectively. This study was approved by the Ethics Committee of Tongji Medical College of Huazhong University of Science and Technology (No. S377). The informed consent was waived by the Ethics Committee for this retrospective study. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013).

EGFR mutation analysis

The overall distribution of EGFR mutations is presented in . EGFR mutations were analyzed according to the principle of the amplified drug resistance mutation system (ARMS). Primary tumors, lymph nodes, distant metastases or pleural effusion specimens were excised, aspirated or biopsied followed by 10% neutral buffered formalin fixation and paraffin embedding. DNA was extracted from formalin-fixed paraffin-embedded tissue sections and analyzed using the Qiagen FFPE Tissue Kit (Netherlands, Roots, NV) according to the manufacturer’s instructions. PCR was performed using the Mx3000PtM System (Stratagene, La Jolla, CA, USA) and the EGFR 29 Mutations Detection Kit (Amoy Diagnostics, Xiamen, People’s Republic of China), and the results were interpreted according to the manufacturer’s instructions.

VENTANA ALK immunohistochemical (IHC) assay

VENTANA is a fully automated IHC detection method based on the D5F3 monoclonal antibody. The VENTANA IHC assay has been approved by the US FDA and China FDA for the identification of patients with non-small cell lung cancer (NSCLC) who are eligible for treatment with ALK tyrosine kinase inhibitors (TKIs). Formalin-fixed paraffin-embedded tissue sections with a thickness of 4 µm were cut according to the manufacturer's instructions and scored using a scoring algorithm. The presence of any percentage of positive tumor cells with strong granular cytoplasmic staining was regarded as ALK positivity, while all other observations were regarded as ALK negativity.

CT image acquisition

CT was performed at our institution using a multislice spiral CT system (SOMATOM Definition AS +, Siemens Healthineers, Germany). Scanning was performed from the level of the chest inlet to the inferior level of the costophrenic angle. The CT parameters were as follows: detector collimation width, 128×0.6 mm; and tube voltage, 120 kV. The tube current was regulated by an automatic exposure control system (CARE Dose 4D). The image thickness and interval were both 5 mm, and the matrix size was 512×512. Thin-slice reconstruction (1.5 mm) and image postprocessing were performed after scanning.

Two radiologists with different degrees of experience in interpreting chest CT images independently performed all qualitative image analyses. One was a senior radiologist with 10 years of experience in thoracic imaging, and the other was a fellow with 4 years of experience in the interpretation of CT images. Both analyzed the Digital Imaging and Communications in Medicine (DICOM) images from the CT studies without access to clinical and histological findings but were aware of the presence and sites of the tumors. If their interpretations differed, the senior reader’s decision was accepted. Data for each CT scan are presented in .

Table 1

CT features for lung adenocarcinoma

Variable	Definition
Location	Central, tumor located in the segmental or more proximal bronchi; peripheral, tumor located in the subsegmental bronchi or more distal airway
Tumor size	Longest diameter of the tumor
Texture	Solid or ground grass opacity
Shape	Indicated as lobulated, others (round, or oval)
Margin	Evaluated in the lung window, and indicated as smooth, or spiculated
Margin definition	Valuated in the lung window, and indicated as well-defined, or poor-defined
Air bronchogram	Tubelike or branched air structure within the tumor
Bubble-like lucency	The presence of air in the tumor at the time of diagnosis prior to biopsy or treatment
Margins	Evaluated in the lung window, and indicated as smooth, or spiculated
Heterogeneity	Homogeneity and heterogeneity
Pleural retraction	Retraction of the pleura toward the tumor
Pleural effusion	Presence or absence of pleural effusion.
Cavitation	Presence or absence of cavitation
Intramodular calcifications	Presence or absence of calcifications
Peripheral emphysema	Presence or absence of peripheral emphysema
Vascular convergence	Convergence of vessels to the tumor, applied to the peripheral tumors
Enhancement	“mild” =0–20 HU; “moderate” =20–40 HU, “marked” >40 HU
Lymphadenopathy	Presence or absence of lymphadenopathy thoracic lymph nodes (hilar or mediastinal) with short-axis diameter greater than 1 cm

Statistical analysis

Statistical analyses of all data were performed using SPSS software (SPSS 21.0 for Windows, IBM, Chicago, IL, USA). In the two groups, clinical characteristics (age, sex, smoking history and TNM stage) were described on a patient basis. CT findings, EGFR status and ALK status description were described on a lesion basis. Interobserver agreement was assessed by the k coefficient. The normality of the distribution was checked using the Kolmogorov-Smirnov test. Normally distributed data, non-normally distributed data and categorical variables were expressed as the mean ± standard deviation, median (interquartile range) and frequency (percentage), respectively. The independent-sample Student’s t test was used for comparisons of two groups of normally distributed variables, and the Chi-square test was used to compare categorical variables. Pearson correlation analyses were performed for normally distributed variables, and Spearman correlation analyses were performed for non-normally distributed data. A P value <0.05 (two-tailed) was considered statistically significant.

Results

Clinical characteristics

In total, 60 eligible patients with synchronous malignancies (40 SDPLA and 20 IPM) were included. The mean age of the SDPLA patients (15 males and 25 females) was 57±7 years. Other patient characteristics, including smoking status, are presented in . No significant differences were found between the two groups in terms of age (57±7 vs. 55±10 years, P=0.336), sex (P=1.000) or smoking history (P=0.541). Regarding clinical staging, 19 patients with SDPLA (47.5%) presented with stage IIIb or IV disease, while most patients with IPM (85%) had an advanced TNM stage (IIIb–IV) ().

Table 2

Comparison of clinical features between the two groups

Variable	SDPLA (n=40)	IPM (n=20)	P values
Age (years)	57±7	55±10	0.336
Sex			1.000
Male	15	8
Female	25	12
Smoking history			0.541
Yes	12	4
No	28	16
Stage&			–
Ia	0	0
Ib	0	0
IIa	0	0
IIb	9	0
IIIa	12	3
IIIb	2	2
IV	17	15

&, according to the IASLC 8th TNM Lung Cancer Staging System. SDPLA, synchronous double primary lung adenocarcinoma; IPM, intrapulmonary metastasis; EGFR, epidermal growth factor receptor; ALK, anaplastic large-cell lymphoma kinase.

Histopathology, EGFR and ALK results

Regarding tumor histology, SDPLA tumors were more frequently associated with acinar predominant subtypes (42.5%) similar to IPM tumors (60%). When tumors were divided into lepidic predominant adenocarcinomas (adenocarcinoma in situ, minimally invasive adenocarcinoma and lepidic predominant invasive adenocarcinoma) and other subtypes of dominant histological findings (acinar, papillary, micropapillary and solid predominant adenocarcinoma as well as variants of invasive adenocarcinoma), SDPLA tumors were more likely to be lepidic predominant adenocarcinomas than IPM tumors (25% vs. 0, P<0.001). Among the 40 cases of SDPLA, 28 patients with SDPLA had at least one tumor exhibiting EGFR mutations (70%). Among 80 tumors of the 40 patients with SDPLA, 45 lesions showed EGFR mutations (56.3%). The incidence of EGFR mutations in this series was 49.2%, whereas patients with SDPLA were more likely to have EGFR mutations than patients with IPM (56.3% vs. 35%, P=0.028). In addition, the discordance rate of EGFR mutations was significantly higher in patients with SDPLA than in those with IPM (40% vs. 5%, P<0.001) () (). The incidence of ALK-positive status in our series was 15%, and patients with IPM were more likely to be ALK-positive than patients with SDPLA (35% vs. 5%, P<0.001) () ().

Table 3

Comparison of pathology and gene status between tumors of two groups

Variable	SDPLA (n=40)	IPM (n=20)	P values
Histological subtype
Lepidic predominant	20	0	<0.001*
Others subtypes	60	40
Acinar predominant	34	24	–
Papillary predominant	15	12	–
Solid predominant	7	6	–
EGFR			0.028*
Mutation	45	14
Wild-type mutation	35	26
EGFR mutation			<0.001*
Identical	24	19
Discrepancy	16	1
ALK expression			<0.001*
Positive	4	14
Negative	76	26

*, P values were based on comparisons between the two groups. SDPLA, synchronous double primary lung adenocarcinoma; IPM, intrapulmonary metastasis; EGFR, epidermal growth factor receptor; ALK, anaplastic large-cell lymphoma kinase.

Interobserver agreement of CT interpretation

The concordance between the two observers was almost perfect with k coefficients ranging between 0.65 and 1.0 ().

Table 4

Analysis of inter-reader agreement percent of concordance and kappa of agreement

CT features	N (% of concordance)	Kappa (95% CI)	Kappa interpretation
Shape	148/160	0.77 (0.69–0.84)	Substantial
Size	154/160	0.90 (0.86–0.93)	Almost perfect
location	156/160	0.95 (0.94–1.00)	Almost perfect
Texture	153/160	0.88 (0.79–0.96)	Almost perfect
Bubblelike lucency	147/160	0.77 (0.69–0.84)	Substantial
Margins	143/160	0.73 (0.69–0.76)	Substantial
Vascular convergence	152/160	0.87 (0.78–0.93)	Almost perfect
Air bronchogram	147/160	0.77 (0.69–0.84)	Substantial
Cavitation	159/160	0.98 (0.96–1.00)	Almost perfect
Pleural retraction	153/160	0.88 (0.79–0.96)	Almost perfect
Spiculate	154/160	0.94 (0.68–0.82)	Almost perfect
Calcifications	151/160	0.86 (0.84–0.89)	Almost perfect
Enhancement degree	156/160	0.96 (0.93–1.00)	Almost perfect
Lymphadenopathy	153/160	0.89 (0.85–0.91)	Almost perfect
Heterogeneity	140/160	0.68 (0.65–0.72)	Substantial

CT signs

Compared with IPM, SDPLA was more frequently located in different lobes (P=0.024), presented less frequently with lymphadenopathy (P=0.014) and showed a smaller difference (Δd) in the largest diameter between tumors (P=0.001). Tumors in the SDPLA group more frequently presented as GGOs (P=0.001) (), lobulated (P<0.001) or spiculated (P<0.001) masses and air bronchograms (P=0.020) (), while tumors in the IPM groups more frequently presented as round/oval solid nodules () with smooth margins on CT images. The incidences of other CT signs were similar between the two groups ().

Figure 2

A 61-year-old female with double primary lung adenocarcinomas with one in the right upper lobe (Tumor A, A) and one in the right lower lobe (Tumor B, D). Tumor A appeared as a mixed ground grass opacity (mGGO) with a lobulated border on CT. Tumor B appeared as a pure ground grass opacity (pGGO) on CT. Hematoxylin-eosin staining (B,E) showed different histological types of adenocarcinoma (×100), and the ARMS method (C,F) revealed a 19_del mutation within exon 19 of the epidermal growth factor receptor (EGFR) gene in Tumor A but not in Tumor B.

Figure 3

A 53-year-old female with double primary lung adenocarcinomas with one in the right middle lobe (Tumor A, A) and one in the right middle lower lobe (Tumor B, D). Tumor A appeared as a pure ground-glass opacities (GGOs) with air bronchogram. Tumor B appeared as a solid nodule with pleural retraction on CT. Hematoxylin-eosin staining (B,E) showed different histological types of adenocarcinoma (×100), and the ARMS method (C,F) revealed a L858R mutation within exon 21 of the epidermal growth factor receptor (EGFR) gene in Tumor A but not in Tumor B.

Figure 4

A 73-year-old male with one primary lung adenocarcinoma in the left upper lobe (A) and one metastasis in the left lower lobe of the same histological type (HE, ×200) and same epidermal growth factor receptor (EGFR) mutation status.

Table 5

Comparison of CT features between SDPLA and IPM

Characteristic	SDPLA (n=40)	IPM (n=20)	P values
Tumor location			0.024*
Same lobe	11	12
Different lobe	29	8
Size difference#	1.1±0.9	2.4±1.9	0.001*
Texture			0.001*
Solid	47	37
GGO	33	3
Shape			<0.001*
Lobulated	63	9
Round/oval	17	31
Margin			<0.001*
Smooth	22	26
Spiculate	58	14
Margin definition			0.838
Well-defined	54	25
Poorly-defined	26	15
Air bronchogram			0.020*
Yes	15	1
No	65	39
Pleural attachment			0.100
Yes	31	9
No	49	31
Bubble-like lucency			0.138
Yes	12	2
No	68	38
Vascular convergence			0.542
Yes	20	8
No	60	32
Cavitation			0.605
Yes	6	2
No	74	38
Calcifications			1.000
Yes	4	2
No	76	38
Lymphadenopathy			0.014*
Yes	7	10
No	33	10
Heterogeneity			0.114
Yes	28	20
No	52	20

*, P values were based on comparisons between the two groups; #, the maximum diameter difference (Äd) between the two lesions. SDPLA, synchronous double primary lung adenocarcinoma; IPM, intrapulmonary metastasis; GGO, ground grass opacity.

Discussion

The differentiation of SDPLA and IPM is clinically important. However, clinical and imaging criteria for differential diagnosis are not well established. In this study, we identified that SDPLA groups had higher discordance rates of EGFR mutations and less commonly ALK-positive mutations compared to IPM patients. CT characteristics were found to be significantly different between SDPLA and IPM.

Patients with SDPLA (52.5%) typically had two early-stage tumors (I–IIIa), while most of those with IPM (85%) had an advanced TNM stage (IIIb–IV). This finding was consistent with the results of a previous study (7). Moreover, regarding tumor histology, SDPLA tumors were more likely to be lepidic predominant adenocarcinomas than IPM tumors. These findings indicated that patients with SDPLA were at an earlier stage than those with IPM and that their cancer was potentially curable.

TKIs have shown remarkable therapeutic effects and enable prolonged progression-free survival (PFS) in NSCLC patients with EGFR mutations or ALK rearrangements (12,13). These discoveries have led to the recommendation of molecular profiling as the standard of care for advanced NSCLC patients. EGFR mutations have been found in adenocarcinomas in female nonsmokers (19,20), and the mutation rate has been reported to be 27–56% in Asian patients (19-21). The incidence of EGFR mutations in the present study was 49.2%, whereas SDPLA tumors were more likely to have EGFR (56.3%) mutations than IPM tumors (35%). This may be due to ethnic differences in the study population and the diagnostic procedures used. Many studies have found that EGFR gene mutations have a high inconsistency rate (80–92.1%) in multiple primary NSCLC, especially when the lesion appears as multiple ground-glass nodules (22,23). The inconsistency rate of EGFR mutations in the present study was significantly higher in patients with SDPLA than in patients with IPM. However, the overall EGFR mutation inconsistency rate in SDPLA was 40%, which was much lower than that reported in previous studies (22,23). Such a distinction may have been observed because EGFR mutation inconsistency is more common in cases with multiple GGOs, while the incidence of GGOs in our study was low (30%). One patient in a previous study had a diagnosis of SDPLA based on differences in EGFR mutation status, while the pathological types of the tumors were similar (5). However, it is not reliable to distinguish SDLA from IPM merely on EGFR mutation status because discordance of EGFR mutations between primary tumors and their corresponding metastases in pulmonary adenocarcinoma has been observed (24,25).

ALK is a tyrosine kinase receptor that shows a dramatic response and prolongs PFS with ALK TKI treatment in NSCLC patients (13). ALK rearrangements have a low incidence (3% to 5% of NSCLC cases) (26) and occur more frequently in young, never-smoker patients with clinically advanced lung adenocarcinoma (27). Studies have reported that ALK-positive tumors are more invasive NSCLCs and are associated with a larger volume, lymph node metastasis or distant metastasis, indicating poor prognosis (28,29). In our data, the rate of ALK-positive SDPLA was 5% similar to the rate of single pulmonary adenocarcinoma (26). Therefore, the EGFR mutation status of SDPLA in the same patient may be independent without mutual interference. However, patients with IPM had a much higher ALK-positive rate (35%). As patients with IPM (85%) typically had a more advanced TNM stage (IIIb–IV) in our study, ALK positivity may be related to advanced tumor status and metastatic status.

Regarding CT signs in the present study, SDPLA tumors occurred more frequently in different lung lobes than IPM tumors, which was consistent with previous studies (9). This phenomenon is related to the fact that metastasis commonly occur sin the same lobe and ipsilateral to the primary lesion. Some studies have supported the lack of characteristic imaging findings in SDPLA (10,15). Dijkman et al. (7) found that a definite diagnosis based on CT morphological features can be made in only 50% of patients (8/16) in the second primary group. However, in the present study, SDPLA more commonly presented as GGOs, while IPM more frequently presented as solid nodules. This might be related to the fact that GGOs are considered multiple primary lung cancers and are detected at an early stage (30,31). Li et al. (16) reported that multiple synchronous lesions commonly present as single or multiple subsolid nodules. Moreover, as mentioned earlier, SDPLA patients were more likely to have EGFR (56.3%) mutations than IPM patients (35%), while patients with IPM had a much higher rate (35%) than SDPLA (5%). Previous data (21) have shown that the occurrence of GGOs is significantly associated with EGFR mutations and that the presence of solid nodules is one of the distinctive CT features of ALK rearrangement lung adenocarcinomas (32). Therefore, these results may also account for the potential association between EGFR and GGO as well as the association between ALK and solid nodules. In the present study, SDPLA more frequently presented as lobulated, spiculated and air bronchograms than IPM, which was consistent with previous studies (16), while IPM more frequently presented as round/oval solid nodules with smooth margins on CT images. These findings indicated that each SDPLA lesion tended to have the malignant characteristics of primary lung cancer. Moreover, IPM patients showed lymph node enlargement more frequently than SDPLA patients, which was confirmed as lymph node metastasis by surgery and was associated with the higher tumor stage of IPM.

Our study had some limitations. (I) This study was performed at a single center with a small series of cases; thus, multicenter studies with large sample sizes are needed to verify our conclusions. (II) This study was a retrospective analysis, and although we strictly used double-blind methods to record CT signs, EGFR gene mutations and ALK results, selection bias was inevitable. (III) We classified the tumors by histological features, which were not verified by molecular biology. (IV) In this study, the prognosis between the two groups was not provided because the cases were collected from 2012 to 2018 and the follow-up time was insufficient.

In conclusion, CT features, EGFR mutations and ALK status may help differentiate SDPLA from IPM. Our preliminary results encourage the establishment of comprehensive diagnostic criteria, including clinical characteristics, histological features, CT images and gene analysis in further study.

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