| Literature DB >> 33206314 |
Carmen Díaz-Galindo1, Denisse Calderón-Vallejo1,2, Irma Hernández-Jasso1, Daniel Cervantes-García3, Diego Martínez-Díaz1, David Ibarra-Martínez2, Martín Muñoz-Ortega4, J Luis Quintanar5.
Abstract
The expression of the gonadotrophin-releasing hormone receptor expression on pituitary gonadotrophs in humans is well characterized. In nervous system they have also been found in hippocampi and cerebral cortex. However, gonadotrophin-releasing hormone receptor expression in human spinal cord has not been reported. This study was to analyze the gonadotrophin-releasing hormone receptor expression in human spinal cord by immunohistochemistry, mRNAs by reverse transcriptase polymerase chain reaction, cDNA cloning and Western blot. The results show immunoreactive material to gonadotrophin-releasing hormone receptor in motoneurons of the spinal cord. Further, the study revealed that spinal cord expressed the gonadotrophin-releasing hormone receptor mRNA. The amplicon sequence corresponds to 100% of identity to GenBank. In Western blot, a band of 37 kDa were found in extracts of spinal cord and placenta as a control. In conclusion, human spinal cord expresses gonadotrophin-releasing hormone receptor analyzed through immunohistochemistry, the expression of its mRNA, cloning its cDNA and Western blot analysis. The presence of gonadotrophin-releasing hormone receptor in the spinal cord suggests the possibility of an extrapituitary functional role independent of reproductive system.Entities:
Keywords: GnRH; Human GnRH-R; Human spinal cord; Immunohistochemistry; RT-PCR
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Year: 2020 PMID： 33206314 DOI： 10.1007/s11064-020-03178-w
Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996