Luciana S Branco-de-Almeida1,2, Yenisel Cruz-Almeida3, Yandy Gonzalez-Marrero1, Rachad Kudsi4, Izabel C V de Oliveira2, Bogdan Dolia1, Hong Huang1, Ikramuddin Aukhil1, Peter Harrison1,5, Luciana M Shaddox1,4. 1. Department of Periodontology, College of Dentistry, University of Florida, Gainesville, Florida, USA. 2. Post Graduate Program in Dentistry, School of Dentistry, Federal University of Maranhão, São Luís, Brazil. 3. Pain Research & Intervention Center of Excellence, University of Florida, Gainesville, Florida, USA. 4. Division of Periodontology, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA. 5. Department of Periodontology, Trinity College School of Dental Sciences, Dublin, Ireland.
Abstract
AIM: To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. METHODS: Sixty-six African-Americans with LAP (7-21 years old) were included. After periodontal examination, all patients received periodontal treatment with mechanical debridement plus systemic amoxicillin/metronidazole for 7 days. Gingival crevicular fluid was collected from diseased and healthy sites at baseline and 3, 6, 12, and 24 months following treatment. Levels of 16 inflammatory/bone resorption markers were determined using Milliplex® . Univariate and correlation analyses were performed among all parameters/biomarkers. Discriminant analyses (DA) evaluated profile differences between LAP diseased and healthy sites at each time point as compared to the baseline. RESULTS: Reductions in the clinical parameters (except for visible plaque) were observed at all time points compared to the baseline. Levels of IL-12p70, IL-2, IL-6, MIP-1α, RANKL, and OPG were reduced after treatment, and several cytokines/chemokines were correlated with clinical parameters reductions. DA showed that differences in the immunoinflammatory profiles between LAP diseased and healthy sites decreased after periodontal treatment compared to the baseline. CONCLUSIONS: Periodontal treatment modified the local immunoinflammatory profile of LAP sites in the long term, as suggested by changes in biomarkers from baseline, along with clinical stability of the disease. (Clinicaltrials.gov number, NCT01330719).
AIM: To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. METHODS: Sixty-six African-Americans with LAP (7-21 years old) were included. After periodontal examination, all patients received periodontal treatment with mechanical debridement plus systemic amoxicillin/metronidazole for 7 days. Gingival crevicular fluid was collected from diseased and healthy sites at baseline and 3, 6, 12, and 24 months following treatment. Levels of 16 inflammatory/bone resorption markers were determined using Milliplex® . Univariate and correlation analyses were performed among all parameters/biomarkers. Discriminant analyses (DA) evaluated profile differences between LAP diseased and healthy sites at each time point as compared to the baseline. RESULTS: Reductions in the clinical parameters (except for visible plaque) were observed at all time points compared to the baseline. Levels of IL-12p70, IL-2, IL-6, MIP-1α, RANKL, and OPG were reduced after treatment, and several cytokines/chemokines were correlated with clinical parameters reductions. DA showed that differences in the immunoinflammatory profiles between LAP diseased and healthy sites decreased after periodontal treatment compared to the baseline. CONCLUSIONS: Periodontal treatment modified the local immunoinflammatory profile of LAP sites in the long term, as suggested by changes in biomarkers from baseline, along with clinical stability of the disease. (Clinicaltrials.gov number, NCT01330719).
Authors: L S Branco-de-Almeida; Y Cruz-Almeida; Y Gonzalez-Marrero; H Huang; I Aukhil; P Harrison; S M Wallet; L M Shaddox Journal: JDR Clin Trans Res Date: 2017-04-14
Authors: N Soedarsono; D Rabello; H Kamei; D Fuma; Y Ishihara; M Suzuki; T Noguchi; Y Sakaki; A Yamaguchi; T Kojima Journal: J Periodontal Res Date: 2006-10 Impact factor: 4.419