Yongji Yan1, Hongfeng Zhang2, Chunfan Jiang3, Xin Ma4, Xueying Zhou5, Xun Tian5,6, Yuping Song7, Xu Chen8, Liyao Yu5, Rui Li5, Hongwei Chen5, Xin Wang6, Ting Liu5, Zhaohui He9, Hongzhao Li4. 1. Department of Urology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. 2. Department of Pathology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. 3. Department of Pathology, Xiangyang Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei, China. 4. Department of Urology, General Hospital of the People's Liberation Army, Beijing, China. 5. Academician expert workstation of Obstetrics and Gynecology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. 6. Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. 7. East Lake Hospital, Wuhan, Hubei, China. 8. Department of Urology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. 9. Department of Urology, Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China.
Abstract
BACKGROUND: Human papillomavirus (HPV) infection is associated with multiple types of cancer, but the evidence has not yet been fully elucidated in bladder cancer. METHODS: Frozen tissue samples collected from 146 patients aged 32 to 89 years with bladder cancer pathological diagnosis between 2015 and 2019 were analyzed. HPV genotyping and integration status determination were performed by capture-based next generation sequencing. Statistical analysis of HPV type distributions was performed according to stage, grade, sex, and age group of patients. RESULTS: Mean (SD) age of the 146 patients was 66.64 ± 10.06 years and 83.56% were men. Overall HPV infection rate was 28.77% (37.50% in women and 27.05% in men), with 11.90% HPV integration events. Among them, 17.12% single and 11.65% coinfections were observed. HPV18 (24.66%) was the most prevalent genotype, followed by HPV33, 16, and 39. All HPV were European lineage (A). HPV16 was more prevalent in women (P = .04). CONCLUSIONS: HPV infection may contribute to the etiology both in men and women with bladder cancer. HPV18, followed by HPV33, 16, and 39 genotypes, potentially represent the predominant oncogenic risk types for bladder carcinogenesis.
BACKGROUND: Human papillomavirus (HPV) infection is associated with multiple types of cancer, but the evidence has not yet been fully elucidated in bladder cancer. METHODS: Frozen tissue samples collected from 146 patients aged 32 to 89 years with bladder cancer pathological diagnosis between 2015 and 2019 were analyzed. HPV genotyping and integration status determination were performed by capture-based next generation sequencing. Statistical analysis of HPV type distributions was performed according to stage, grade, sex, and age group of patients. RESULTS: Mean (SD) age of the 146 patients was 66.64 ± 10.06 years and 83.56% were men. Overall HPV infection rate was 28.77% (37.50% in women and 27.05% in men), with 11.90% HPV integration events. Among them, 17.12% single and 11.65% coinfections were observed. HPV18 (24.66%) was the most prevalent genotype, followed by HPV33, 16, and 39. All HPV were European lineage (A). HPV16 was more prevalent in women (P = .04). CONCLUSIONS:HPV infection may contribute to the etiology both in men and women with bladder cancer. HPV18, followed by HPV33, 16, and 39 genotypes, potentially represent the predominant oncogenic risk types for bladder carcinogenesis.
Authors: Narcisa Muresu; Biagio Di Lorenzo; Laura Saderi; Illari Sechi; Arcadia Del Rio; Andrea Piana; Giovanni Sotgiu Journal: Diagnostics (Basel) Date: 2022-07-20