Camilla Dos Santos Tibúrcio-Machado1, Pauline Mastella Lang2, Maria Martha Campos3, Carlos Frederico Brilhante Wolle4, Raquel Cristine Silva Barcelos5, Natália Brezolin Zago6, Carlos Eduardo Leite7, Adriana Etges8, Carlos Alexandre Souza Bier6. 1. Graduate Program in Dental Science, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil. Electronic address: camilla_tiburcio@hotmail.com. 2. Undergraduate Program in Dentistry, Universidade Regional Integrada do Alto Uruguai e das Missões (URI), Erechim, Rio Grande do Sul, Brazil. 3. Faculty of Dentistry/Institute of Toxicology and Pharmacology, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), RS, Brazil. Electronic address: maria.campos@pucrs.br. 4. Uningá Eleva, Santa Maria, RS, Brazil. Electronic address: carlosfbwolle@ibest.com.br. 5. Graduate Program in Dental Science, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil. 6. Graduate Program in Dental Science, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil. 7. Undergraduate Program in Dentistry and Nursing, IMED/RS, Porto Alegre, Rio Grande do Sul, Brazil. 8. Oral Pathology Department, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
Abstract
AIMS: To evaluate the effects of a high-fat diet (HFD) on the progression of apical periodontitis (AP), local inflammation, systemic antioxidant status, and blood lipid profile in rats. MAIN METHODS: Sixteen male Wistar rats were fed a standard diet (SD) or a HFD. At the sixth experimental week, the pulp chambers of the mandibular first molars were exposed to develop AP. A glucose tolerance test was performed the week before euthanasia. At the tenth experimental week, the animals were euthanized and the livers were collected to estimate catalase (CAT) and reduced glutathione (GSH) levels. Blood was acquired for biochemical analysis. The size of AP was estimated from radiographs and described as AP size-to-body weight ratio; inflammatory grade of AP was determined by histological analysis. KEY FINDINGS: At the end of the experimental period, the rats fed the HFD had 30% less weight (P < 0.0001) and higher blood glucose levels after 30 min of sucrose intake (P < 0.05) than those fed the SD. Animals from the HFD group had lower levels of CAT (P < 0.01), but the same was not observed in the GSH levels. Plasma insulin and total cholesterol were not affected by the diet. The rats fed the HFD presented greater AP than those fed the SD (P < 0.05). However, the local inflammatory infiltrate was similar in both groups. SIGNIFICANCE: The alterations promoted by the consumption of a HFD were not only observed systemically, but also locally, producing greater AP in rats than a SD.
AIMS: To evaluate the effects of a high-fat diet (HFD) on the progression of apical periodontitis (AP), local inflammation, systemic antioxidant status, and blood lipid profile in rats. MAIN METHODS: Sixteen male Wistar rats were fed a standard diet (SD) or a HFD. At the sixth experimental week, the pulp chambers of the mandibular first molars were exposed to develop AP. A glucose tolerance test was performed the week before euthanasia. At the tenth experimental week, the animals were euthanized and the livers were collected to estimate catalase (CAT) and reduced glutathione (GSH) levels. Blood was acquired for biochemical analysis. The size of AP was estimated from radiographs and described as AP size-to-body weight ratio; inflammatory grade of AP was determined by histological analysis. KEY FINDINGS: At the end of the experimental period, the rats fed the HFD had 30% less weight (P < 0.0001) and higher blood glucose levels after 30 min of sucrose intake (P < 0.05) than those fed the SD. Animals from the HFD group had lower levels of CAT (P < 0.01), but the same was not observed in the GSH levels. Plasma insulin and total cholesterol were not affected by the diet. The rats fed the HFD presented greater AP than those fed the SD (P < 0.05). However, the local inflammatory infiltrate was similar in both groups. SIGNIFICANCE: The alterations promoted by the consumption of a HFD were not only observed systemically, but also locally, producing greater AP in rats than a SD.