Literature DB >> 3319844

Monoclonal antibodies against pig transferrin. Blocking and binding activity.

I Trebichavský1, B Macků, V Viklický, M Koukal, P Siman.   

Abstract

Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Only the PTF-03 antibody can be used for immunofluorescence detection of pig transferrin bound to specific receptors on the plasma membrane. The binding of iodinated pig transferrin to PK cells was studied. It could be blocked by non-labelled transferrin in excess, by pig serum or by anti-pig transferrin monoclonal antibodies. PTF-03 expressed the lowest blocking activity among the antibodies tested.

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Year:  1987        PMID： 3319844     DOI： 10.1007/BF02887578

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  9 in total

1.  Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.

Authors:  J Bártek; V Viklický; A Stratil
Journal:  Br J Haematol       Date:  1985-03       Impact factor: 6.998

2.  The role of iron and transferrin in lymphocyte transformation.

Authors:  J H Brock; T Mainou-Fowler
Journal:  Immunol Today       Date:  1983-12

3.  A method of trace iodination of proteins for immunologic studies.

Authors:  P J McConahey; F J Dixon
Journal:  Int Arch Allergy Appl Immunol       Date:  1966

4.  Transferrin binding by mitogen-activated human peripheral blood lymphocytes.

Authors:  G M Galbraith; J M Goust; S M Mercurio; R M Galbraith
Journal:  Clin Immunol Immunopathol       Date:  1980-08

Review 5.  Iron transport and storage proteins.

Authors:  P Aisen; I Listowsky
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

6.  Production and characterization of monoclonal antibodies against human transferrin.

Authors:  J Bártek; V Viklický; V Horejsí; H Verlová; P Dráber
Journal:  Folia Biol (Praha)       Date:  1984       Impact factor: 0.906

7.  Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.

Authors:  I S Trowbridge; J Lesley; R Schulte
Journal:  J Cell Physiol       Date:  1982-09       Impact factor: 6.384

8.  Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity.

Authors:  J Bártek; V Viklický; F Franĕk; P Angelisová; P Dráber; T Jarosíková; M Nĕmec; H Verlová
Journal:  Immunol Lett       Date:  1982-05       Impact factor: 3.685

9.  The effect of trypsin digestion on the structure and iron-donating properties of transferrins from several species.

Authors:  I Esparza; J H Brock
Journal:  Biochim Biophys Acta       Date:  1980-04-25
  9 in total

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