Fei Xia1, Jing Zhao1, Dan Fu1, Ye Xu1, Peijun Yao1, Meiyan Li1, Aruma Aruma1, Xingtao Zhou2. 1. Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. 2. Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. Electronic address: doctzhouxingtao@163.com.
Abstract
PURPOSE: To investigate the optical transmittance and ultrastructure of small incision lenticule extraction (SMILE)-derived lenticules preserved via three different methods. METHODS: A total of 90 lenticules obtained from myopic patients undergoing SMILE surgery were divided into control and experimental groups. Fresh lenticules served as the control. The preserved lenticules of the experimental group were randomly divided into three subgroups according to different storage conditions: anhydrous glycerol, silicone oil and allochroic silica gel groups. Optical transmittance was evaluated, histological changes were analysed by haematoxylin eosin (HE) staining, and collagen fibril densities and necrotic response were assessed via transmission electron microscopy (TEM) at 48 h, 14 days and 4 weeks. RESULTS: After storage for 4 weeks, the mean percentage transmittance values in glycerol and silicone oil groups significantly decreased (P = 0.034 and P = 0.042, respectively), but the lenticules preserved in silica gel remained unchanged when compared with the control lenticules. In all the groups, HE staining results showed a regular arrangement of collagen fibers with a few keratocytes and several cavitation bubbles. TEM revealed that the fibril densities in the glycerol group (273.70 ± 31.42/μm2) after 4 weeks were significant less than those in the other two groups (silicone oil, 335.20 ± 33.09/μm2; silica gel, 371.80 ± 37.60/μm2) and control group (340 ± 33.61/μm2) (all P < 0.001). In each group, a few necrotic and apoptotic keratocytes were observed. CONCLUSIONS: All the three agents, namely glycerol, silicone oil and silica gel, could be used for lenticule preservation. Silica gel facilitates better maintenance of optical transmittance than the other two agents.
PURPOSE: To investigate the optical transmittance and ultrastructure of small incision lenticule extraction (SMILE)-derived lenticules preserved via three different methods. METHODS: A total of 90 lenticules obtained from myopic patients undergoing SMILE surgery were divided into control and experimental groups. Fresh lenticules served as the control. The preserved lenticules of the experimental group were randomly divided into three subgroups according to different storage conditions: anhydrous glycerol, silicone oil and allochroic silica gel groups. Optical transmittance was evaluated, histological changes were analysed by haematoxylin eosin (HE) staining, and collagen fibril densities and necrotic response were assessed via transmission electron microscopy (TEM) at 48 h, 14 days and 4 weeks. RESULTS: After storage for 4 weeks, the mean percentage transmittance values in glycerol and silicone oil groups significantly decreased (P = 0.034 and P = 0.042, respectively), but the lenticules preserved in silica gel remained unchanged when compared with the control lenticules. In all the groups, HE staining results showed a regular arrangement of collagen fibers with a few keratocytes and several cavitation bubbles. TEM revealed that the fibril densities in the glycerol group (273.70 ± 31.42/μm2) after 4 weeks were significant less than those in the other two groups (silicone oil, 335.20 ± 33.09/μm2; silica gel, 371.80 ± 37.60/μm2) and control group (340 ± 33.61/μm2) (all P < 0.001). In each group, a few necrotic and apoptotic keratocytes were observed. CONCLUSIONS: All the three agents, namely glycerol, silicone oil and silica gel, could be used for lenticule preservation. Silica gel facilitates better maintenance of optical transmittance than the other two agents.
Authors: Martina Nemcokova; Jakub Dite; Yun Min Klimesova; Magdalena Netukova; Pavel Studeny Journal: Cell Tissue Bank Date: 2022-02-06 Impact factor: 1.522
Authors: Gary Hin-Fai Yam; Francisco Bandeira; Yu-Chi Liu; Kavya Devarajan; Nur Zahirah Binte M Yusoff; Hla-Myint Htoon; Jodhbir S Mehta Journal: J Adv Res Date: 2021-09-16 Impact factor: 12.822