Removal of pathogen and antibiotic resistance genes from waste activated sludge by different pre-treatment approaches.

In wastewater treatment plants, most of the pathogens and antibiotic resistant genes (ARGs) transferred into and concentrated in waste activated sludge (WAS), which would cause severe public health risks. In this study, the capabilities of several WAS pre-treatment approaches to inactivate coliforms/E. coli and ARGs, as well as the subsequent regrowth of coliforms/E. coli and ARGs/intI1 in treated sludge were investigated. The results showed that electro-Fenton (EF), with continuous hydroxyl radical generation, could efficiently inactivate coliforms/E. coli in 60 min (about 4 log units), followed by methanol (MT), anode oxidization (AO), and acidification (AT). Kinetic analysis showed that the inactivation mainly occurred in the first 10 min. However, the efficiencies of all studied pre-treatment approaches on inactivating ARGs/intI1 (<2 log units) were lower than coliforms/E. coli, whilst EF still had the highest efficiency of ARGs/intI1 reduction. Mechanical ultrasound treatment (ULS) could not inactivate coliforms/E. coli in WAS, but it could efficiently reduce ARGs/intI1. High regrowth rates of coliforms/E. coli were observed in the treated WAS in 10 days, but the abundances of ARGs/intI1 continuously reduced during the after-treatment incubation. Our study showed that EF could efficiently disinfect potential pathogens, however, the reduction of ARGs/intI1 in WAS need further investigation.