Untargeted metabolomics for Achilles heel of engineered nanomaterials' risk assessment.

Owing to the superlative properties, engineered nanomaterials (ENM) are being used in food, cosmetics, medicine, and electronics. Therefore, exogenous ENM can be housed into humans through a multitude of exposure routes, leading to compromise of the biomolecules' functionalities through structural deformations, and even at the metabolic level. Consequently, it is of great importance to understand the perturbations introduced at the metabolic level for the timely risk assessment (RA) of ENM. Current technological advancements in metabolomics empower us to visualize the metabolic dysregulations in biological cells, tissues, and living objects, instigated by the ENM. Given the fact, we propose multitiered untargeted metabolomics for the risk assessment of ENM. We propose largely validated experimental design principles that enable the well-organized and authentic identification of metabolic dysregulation connected with a newly engineered nanomaterial. Our scheme could participate in the enhanced transparency of the RA course of rapidly emerging ENM.