Hariprasath Prakash1, Ponmurugan Karuppiah2, Naif A Al-Dhabi2, Gandham S Prasad3, Chandan Badapanda4, Arunaloke Chakrabarti1, Shivaprakash M Rudramurthy1. 1. Department of Medical Microbiology, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India. 2. Department of Botany & Microbiology, College of Sciences, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. 3. Technology, Industrial Liaison & Entrepreneurship Unit, University of Hyderabad, Hyderabad 500046, Telangana, India. 4. Bioinformatics Division, Xcelris Labs Limited, Ahmedabad 380015, Gujarat, India.
Abstract
Aim: To understand the phylogenomics, pathogenic/virulence-associated genes and genomic evolution of pathogenic Sporothrix species. Materials & methods: We performed in silico comparative genome analysis of Sporothrix species using ab initio tools and in-house scripts. We predicted genes and repeats, compared genomes based on synteny, identified orthologous clusters, assessed genes family expansion/contraction, predicted secretory proteins and finally searched for similar sequences from various databases. Results: The phylogenomics revealed that Sporothrix species are closely related to Ophiostoma species. The gene family evolutionary analysis revealed the expansion of genes related to virulence (CFEM domain, iron acquisition genes, lysin motif domain), stress response (Su[var]3-9, Enhancer-of-zeste and Trithorax domain and Domain of unknown function 1996), proteases (aspartic protease, x-pro dipeptidyl-peptidase), cell wall composition associated genes (chitin deacetylase, chitinase) and transporters (major facilitator superfamily transporter, oligo-peptide transporter family) in Sporothrix species. Conclusion: The present study documents the putative pathogenic/virulence-associated genes in the Sporothrix species.
Aim: To understand the phylogenomics, pathogenic/virulence-associated genes and genomic evolution of pathogenic Sporothrix species. Materials & methods: We performed in silico comparative genome analysis of Sporothrix species using ab initio tools and in-house scripts. We predicted genes and repeats, compared genomes based on synteny, identified orthologous clusters, assessed genes family expansion/contraction, predicted secretory proteins and finally searched for similar sequences from various databases. Results: The phylogenomics revealed that Sporothrix species are closely related to Ophiostoma species. The gene family evolutionary analysis revealed the expansion of genes related to virulence (CFEM domain, iron acquisition genes, lysin motif domain), stress response (Su[var]3-9, Enhancer-of-zeste and Trithorax domain and Domain of unknown function 1996), proteases (aspartic protease, x-pro dipeptidyl-peptidase), cell wall composition associated genes (chitin deacetylase, chitinase) and transporters (major facilitator superfamily transporter, oligo-peptide transporter family) in Sporothrix species. Conclusion: The present study documents the putative pathogenic/virulence-associated genes in the Sporothrix species.
Authors: Laura C García-Carnero; Héctor M Mora-Montes; Nallely Nava-Pérez; Lisset G Neri-García; Oscar E Romero-González; Joshua A Terrones-Cruz Journal: Infect Drug Resist Date: 2022-04-22 Impact factor: 4.177