Mohsen Asouri1,2, Hamid Alinejad Rokni3, Mohammad Ali Sahraian4, Sadegh Fattahi2, Nima Motamed5, Rozita Doosti4, Hamzeh Rahimi1, Maryam Lotfi2, Azam Moslemi6, Morteza Karimpoor1, Fereidoun Mahboudi1, Haleh Akhavan-Niaki7,8. 1. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. 2. North Research Center, Pasteur Institute of Iran, Amol, Iran. 3. Systems Biology and Health Data Analytics Lab, the Graduate School of Biomedical Engineering, UNSW Sydney, 2052, NSW, AU. 4. Multiple Sclerosis Research Center; Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. 5. Department of Social Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. 6. Department of Biostatistics, Faculty of Medical Sciences, Arak University of Medical Sciences, Arak, Iran. 7. Zoonoses Research Center, North Research Center, Pasteur Institute of Iran, Amol, Iran. 8. Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Abstract
BACKGROUND: Multiple sclerosis (MS) is a common demyelinating neurodegenerative disorder with significant heritability. Previous studies have associated genetic variants in human leukocyte antigen (HLA) complex, IL2RA , and HMGB1 genes with the pathophysiology of MS. METHODS: In order to investigate the gene association in the Iranian population, we performed a genotyping study of 36 variants in the mentioned genes using Sanger sequencing in 102 MS patients and 113 healthy controls. RESULTS: Our results identified significant associations as well as significant allele frequency differences in some of the studied single-nucleotide polymorphisms including rs4935356, rs3177928, and rs7197 from HLA-DRA gene, and rs12722489 and rs12722490 variants from IL2RA gene (p< 0.05). Moreover, the strong linkage disequilibrium of two common haplotypes was estimated from the HLA-DRA gene. CONCLUSION: This association study may suggest the role of these polymorphisms in the genetic susceptibility of MS in the Iranian population and would facilitate the recognition of causative variants in this disease.
BACKGROUND: Multiple sclerosis (MS) is a common demyelinating neurodegenerative disorder with significant heritability. Previous studies have associated genetic variants in human leukocyte antigen (HLA) complex, IL2RA , and HMGB1 genes with the pathophysiology of MS. METHODS: In order to investigate the gene association in the Iranian population, we performed a genotyping study of 36 variants in the mentioned genes using Sanger sequencing in 102 MS patients and 113 healthy controls. RESULTS: Our results identified significant associations as well as significant allele frequency differences in some of the studied single-nucleotide polymorphisms including rs4935356, rs3177928, and rs7197 from HLA-DRA gene, and rs12722489 and rs12722490 variants from IL2RA gene (p< 0.05). Moreover, the strong linkage disequilibrium of two common haplotypes was estimated from the HLA-DRA gene. CONCLUSION: This association study may suggest the role of these polymorphisms in the genetic susceptibility of MS in the Iranian population and would facilitate the recognition of causative variants in this disease.
Authors: María L Cavanillas; Antonio Alcina; Concepción Núñez; Virginia de las Heras; Miguel Fernández-Arquero; Manuel Bartolomé; Emilio G de la Concha; Oscar Fernández; Rafael Arroyo; Fuencisla Matesanz; Elena Urcelay Journal: Eur J Hum Genet Date: 2010-02-24 Impact factor: 4.246
Authors: Arnaud Friggeri; Yanping Yang; Sami Banerjee; Yong-Jun Park; Gang Liu; Edward Abraham Journal: Am J Physiol Cell Physiol Date: 2010-09-08 Impact factor: 4.249
Authors: Asa Andersson; Ruxandra Covacu; Dan Sunnemark; Alexandre I Danilov; Assunta Dal Bianco; Mohsen Khademi; Erik Wallström; Anna Lobell; Lou Brundin; Hans Lassmann; Robert A Harris Journal: J Leukoc Biol Date: 2008-07-21 Impact factor: 4.962
Authors: T Berge; I S Leikfoss; I S Brorson; S D Bos; C M Page; M W Gustavsen; A Bjølgerud; T Holmøy; E G Celius; J Damoiseaux; J Smolders; H F Harbo; A Spurkland Journal: Genes Immun Date: 2016-01-14 Impact factor: 2.676