| Literature DB >> 33178823 |
Xu Jia-Yuan1, Song Wei1, Lu Fang-Fang1, Dai Zhi-Jian1, Cao Long-He1, Lin Sen1.
Abstract
[This corrects the article DOI: 10.1155/2020/9704245.].Entities:
Year: 2020 PMID: 33178823 PMCID: PMC7648670 DOI: 10.1155/2020/3595402
Source DB: PubMed Journal: Biomed Res Int Impact factor: 3.411
Figure 1Expression levels of miR-375 and PDK1 in NPC tissues and cell lines were assessed. (a, b) qRT-PCR was performed to measure expression levels of miRNAs and PDK1 in 23 normal samples and in 38 NPC samples. miR-375 is downregulated in NPC tissue, compared with normal tissue, while PDK1 is upregulated in NPC tissue, compared with normal tissue (Student's t-test, ∗∗P < 0.01). (c, d) qRT-PCR was performed to evaluate miR-375 expression in the N69 epithelial normal nasopharynx cell line and NPC cell lines (CNE-1,CNE-2, C666-1, and HONE1). Conversely, levels of PDK1 were markedly increased in NPC cell lines, compared to NP69 (CNE-1, CNE-2, C666-1, and HONE1) (Student's t-test, ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001). Cell lines CNE-2 and CNE-1 showed the greatest difference in miR-375 expression levels when compared to NP69 cells and were used for subsequent analyses. Each assay was conducted in duplicate, three times. Values are presented ±s.e.m.