A Ghalyanchilangeroudi1, H Najafi2, M H Fallah Mehrabadi3, Z Ziafati Kafi4, N Sadri4, A Hojabr Rajeoni4, A Modiri5, A Safari5, H Hosseini6. 1. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 2. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran, and Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran (current address). 3. Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. 4. Ph.D. Student in Virology, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 5. Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 6. Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
Abstract
BACKGROUND: Avian infectious bronchitis (IB) is an infectious viral disease of chickens. The effective protection of chickens against many different infectious bronchitis virus (IBV) variants is not achieved unless the circulating genotypes in the region are identified and the cross-protection of the potential of vaccines in use is assessed. AIMS: In a monitoring program of IBVs, a new genotype was identified in the north of Iran, 2019. This work was conducted to isolate and characterize this new IBV genotype. METHODS: Tracheal tissues were collected from chickens showing signs of respiratory involvement. Specimens were homogenized and inoculated to the allantoic fluid of embryonated specific pathogen-free (SPF) eggs. Infectious bronchitis virus was detected using real time-polymerase chain reaction (RT-PCR). The hypervariable region of the IBV S1 gene was amplified for sequencing. RESULTS: Positive samples were phylogenetically analyzed, and both positive isolates were clustered with Q1 IBV strains. CONCLUSION: This is the first report of the Q1 outbreak in Iran. More investigations are needed to find the role of Q1 IBV in the respiratory disease complex of chickens.
BACKGROUND: Avian infectious bronchitis (IB) is an infectious viral disease of chickens. The effective protection of chickens against many different infectious bronchitis virus (IBV) variants is not achieved unless the circulating genotypes in the region are identified and the cross-protection of the potential of vaccines in use is assessed. AIMS: In a monitoring program of IBVs, a new genotype was identified in the north of Iran, 2019. This work was conducted to isolate and characterize this new IBV genotype. METHODS: Tracheal tissues were collected from chickens showing signs of respiratory involvement. Specimens were homogenized and inoculated to the allantoic fluid of embryonated specific pathogen-free (SPF) eggs. Infectious bronchitis virus was detected using real time-polymerase chain reaction (RT-PCR). The hypervariable region of the IBV S1 gene was amplified for sequencing. RESULTS: Positive samples were phylogenetically analyzed, and both positive isolates were clustered with Q1 IBV strains. CONCLUSION: This is the first report of the Q1 outbreak in Iran. More investigations are needed to find the role of Q1 IBV in the respiratory disease complex of chickens.
Authors: Mohamed H Houta; Kareem E Hassan; Azza A El-Sawah; Magdy F Elkady; Walid H Kilany; Ahmed Ali; Ahmed S Abdel-Moneim Journal: Arch Virol Date: 2021-01-08 Impact factor: 2.574