Marisol Porto Rocha1, Mariana Sousa Santos2, Paôlla Layanna Fernandes Rodrigues3, Thalita Santos Dantas Araújo4, Janeide Muritiba de Oliveira5, Luciano Pereira Rosa6, Vanderlei Salvador Bagnato7, Francine Cristina da Silva8. 1. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: marisolporto87@gmail.com. 2. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: mariana.sousa.19@hotmail.com. 3. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: p.layanna@hotmail.com. 4. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: thalita_sda@hotmail.com. 5. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: janeidemuritiba@yahoo.com.br. 6. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: drlucianorosa@yahoo.com.br. 7. Institute of Physics of São Carlos, University of São Paulo, São Carlos, SP, Brazil. Electronic address: vander@usp.br. 8. Multidisciplinary Institute in Health, Federal University of Bahia - UFBA, Brazil. Electronic address: drfransilva@yahoo.com.br.
Abstract
BACKGROUND: Antimicrobial photodynamic therapy (PDT) has emerged as a therapeutic strategy to conventional procedures using antibiotics. OBJECTIVE: To evaluate the antimicrobial effectiveness of PDT using blue light emitting diode (LED) associated with curcumin on biofilms of Enterococcus faecalis in bovine bone cavities and also to analyze the presence of these biofilms through spectral fluorescence. MATERIALS AND METHODS: Standardized suspensions of E. faecalis (ATCC 29212) were incubated in artificial bone cavities for 14 days at 36 °C ± 1 °C for biofilm formation. The test specimens were distributed among the four experimental groups (n = 10): L-C- (control), L + C- (LED for 5 min), L-C+ (curcumin for 5 min) and L + C+ (PDT). Aliquots were collected from the bone cavities after treatments and seeded on BHI agar for 24 h at 36 °C ± 1 °C for CFU count. Before and after each treatment the specimens were submitted to spectral fluorescence, whose images were compared in the Image J program. The log10 CFU/mL results were submitted to the Kruskal-Wallis test (5%) and the biofilm fluorescence spectroscopy results were submitted to the Wilcoxon test (5%). RESULTS: All treatments presented statistical difference when compared to the control, and PDT was responsible for the largest reduction (1.92 log10 CFU/mL). There was a reduction in the fluorescence emitted after the treatments, with greater statistical difference in the PDT group. CONCLUSION: PDT was efficient in the reduction of E. faecalis biofilms. In all groups post treatment there was a significant reduction of biofilms in the fluorescence spectroscopy images with greater reduction in the PDT group.
BACKGROUND: Antimicrobial photodynamic therapy (PDT) has emerged as a therapeutic strategy to conventional procedures using antibiotics. OBJECTIVE: To evaluate the antimicrobial effectiveness of PDT using blue light emitting diode (LED) associated with curcumin on biofilms of Enterococcus faecalis in bovine bone cavities and also to analyze the presence of these biofilms through spectral fluorescence. MATERIALS AND METHODS: Standardized suspensions of E. faecalis (ATCC 29212) were incubated in artificial bone cavities for 14 days at 36 °C ± 1 °C for biofilm formation. The test specimens were distributed among the four experimental groups (n = 10): L-C- (control), L + C- (LED for 5 min), L-C+ (curcumin for 5 min) and L + C+ (PDT). Aliquots were collected from the bone cavities after treatments and seeded on BHIagar for 24 h at 36 °C ± 1 °C for CFU count. Before and after each treatment the specimens were submitted to spectral fluorescence, whose images were compared in the Image J program. The log10 CFU/mL results were submitted to the Kruskal-Wallis test (5%) and the biofilm fluorescence spectroscopy results were submitted to the Wilcoxon test (5%). RESULTS: All treatments presented statistical difference when compared to the control, and PDT was responsible for the largest reduction (1.92 log10 CFU/mL). There was a reduction in the fluorescence emitted after the treatments, with greater statistical difference in the PDT group. CONCLUSION: PDT was efficient in the reduction of E. faecalis biofilms. In all groups post treatment there was a significant reduction of biofilms in the fluorescence spectroscopy images with greater reduction in the PDT group.