Augusto Rodrigues Lima1,2, Daniel Rodrigo Herrera1,3, Priscila Amanda Francisco1, Andrea Cardoso Pereira1,2, Jose Lemos2, Jacqueline Abranches4, Brenda P F A Gomes5. 1. Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, University of Campinas-UNICAMP, Av Limeira, 901, Bairro Areião, Piracicaba, SP, 13414-903, Brazil. 2. Department of Oral Biology, University of Florida, UF. College of Dentistry, 1395 Center Drive, Box 100424, Gainesville, FL, 32610-0424, USA. 3. Department of Endodontics, Fluminense Federal University - UFF, Niteroi, RJ, Brazil. 4. Department of Oral Biology, University of Florida, UF. College of Dentistry, 1395 Center Drive, Box 100424, Gainesville, FL, 32610-0424, USA. jabranches@dental.ufl.edu. 5. Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, University of Campinas-UNICAMP, Av Limeira, 901, Bairro Areião, Piracicaba, SP, 13414-903, Brazil. bpgomes@fop.unicamp.br.
Abstract
OBJECTIVES: To investigate the presence of Streptococcus mutans in root canals of symptomatic necrotic teeth (SNT) and their associated acute apical abscesses (AAA) and in the root canals of asymptomatic necrotic teeth (ANT). It also aimed to investigate the presence of the cnm and cbm genes in specimens that harbored S. mutans. MATERIALS AND METHODS: DNA was extracted from samples collected from 10 patients presenting pulpal necrosis associated with radiographic evidence of apical periodontitis (ANT) and from 10 patients in need of endodontic therapy due to the presence of pulpal necrosis (SNT) and AAA. The control group consisted of 10 patients with teeth with normal vital pulp and requiring endodontic treatment for prosthetic reasons. The presence of S. mutans was detected by quantitative real-time-PCR (qPCR) using species-specific primers. Samples harboring S. mutans were further evaluated for the presence of CBP genes by qPCR as well. RESULTS: All studied sites showed a high prevalence of S. mutans, except the control group. Specifically, 60% of ANT and 70% of AAA/SNT paired samples were positive for S. mutans. The cnm gene was detected positive for S. mutans only in ANT samples (66.6%). The cbm gene was not detected in any of the investigated sites. CONCLUSIONS: S. mutans was found in high prevalence in both asymptomatic and symptomatic endodontic infections, including in abscesses, but it was not detected in the root canals of teeth with normal vital pulp. Interestingly, cnm+ S. mutans was only detected in asymptomatic/chronic primary endodontic infections associated with apical lesion. Therefore, it appears that cnm, and possibly other CBPs, may play an underestimated role in chronic endodontic infections. CLINICAL RELEVANCE: A high prevalence of Streptococcus mutans cnm+ gene was detected only in asymptomatic primary endodontic infections associated with apical lesion. Therefore, it appears that this collagen-binding protein gene plays an underestimated role in asymptomatic/chronic endodontic infections.
OBJECTIVES: To investigate the presence of Streptococcus mutans in root canals of symptomatic necrotic teeth (SNT) and their associated acute apical abscesses (AAA) and in the root canals of asymptomatic necrotic teeth (ANT). It also aimed to investigate the presence of the cnm and cbm genes in specimens that harbored S. mutans. MATERIALS AND METHODS: DNA was extracted from samples collected from 10 patients presenting pulpal necrosis associated with radiographic evidence of apical periodontitis (ANT) and from 10 patients in need of endodontic therapy due to the presence of pulpal necrosis (SNT) and AAA. The control group consisted of 10 patients with teeth with normal vital pulp and requiring endodontic treatment for prosthetic reasons. The presence of S. mutans was detected by quantitative real-time-PCR (qPCR) using species-specific primers. Samples harboring S. mutans were further evaluated for the presence of CBP genes by qPCR as well. RESULTS: All studied sites showed a high prevalence of S. mutans, except the control group. Specifically, 60% of ANT and 70% of AAA/SNT paired samples were positive for S. mutans. The cnm gene was detected positive for S. mutans only in ANT samples (66.6%). The cbm gene was not detected in any of the investigated sites. CONCLUSIONS: S. mutans was found in high prevalence in both asymptomatic and symptomatic endodontic infections, including in abscesses, but it was not detected in the root canals of teeth with normal vital pulp. Interestingly, cnm+ S. mutans was only detected in asymptomatic/chronic primary endodontic infections associated with apical lesion. Therefore, it appears that cnm, and possibly other CBPs, may play an underestimated role in chronic endodontic infections. CLINICAL RELEVANCE: A high prevalence of Streptococcus mutanscnm+ gene was detected only in asymptomatic primary endodontic infections associated with apical lesion. Therefore, it appears that this collagen-binding protein gene plays an underestimated role in asymptomatic/chronic endodontic infections.
Authors: Francisco Montagner; Rogério C Jacinto; Fernanda G C Signoretti; Paula F Sanches; Brenda P F A Gomes Journal: J Endod Date: 2011-12-16 Impact factor: 4.171
Authors: J J De Soet; M C M van Gemert-Schriks; M L Laine; W E van Amerongen; S A Morré; A J van Winkelhoff Journal: Caries Res Date: 2008-08-14 Impact factor: 4.056