Literature DB >> 3316711

Expression of dengue virus structural proteins and nonstructural protein NS1 by a recombinant vaccinia virus.

B T Zhao1, G Prince, R Horswood, K Eckels, P Summers, R Chanock, C J Lai.   

Abstract

A recombinant vaccinia virus containing cloned DNA sequences coding for the three structural proteins and nonstructural proteins NS1 and NS2a of dengue type 4 virus was constructed. Infection of CV-1 cells with this recombinant virus produced dengue virus structural proteins as well as the nonstructural protein NS1. These proteins were precipitated by specific antisera and exhibited the same molecular size and glycosylation patterns as authentic dengue virus proteins. Infection of cotton rats with the recombinant virus induced NS1 antibodies in 1 of 11 animals. However, an immune response to the PreM and E glycoproteins was not detected. A reduced level of gene expression was probably the reason for the limited serologic response to these dengue virus antigens.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3316711      PMCID： PMC256024          DOI： 10.1128/JVI.61.12.4019-4022.1987

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  17 in total

1.  Properties and antigenic relationships of hemagglutinins associated with the dengue viruses.

Authors:  B H SWEET; A B SABIN
Journal:  J Immunol       Date:  1954-11       Impact factor: 5.422

2.  Physical and biological properties of dengue-2 virus and associated antigens.

Authors:  T J Smith; W E Brandt; J L Swanson; J M McCown; E L Buescher
Journal:  J Virol       Date:  1970-04       Impact factor: 5.103

3.  Studies on the nature of dengue viruses. IV. The structural proteins of type 2 dengue virus.

Authors:  V Stollar
Journal:  Virology       Date:  1969-11       Impact factor: 3.616

4.  Studies on the nature of dengue viruses. I. Correlation of particle density, infectivity, and RNA content of type 2 virus.

Authors:  T M Stevens; R W Schlesinger
Journal:  Virology       Date:  1965-09       Impact factor: 3.616

5.  Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution.

Authors:  C M Rice; E M Lenches; S R Eddy; S J Shin; R L Sheets; J H Strauss
Journal:  Science       Date:  1985-08-23       Impact factor: 47.728

6.  Identification of dengue type 2 virus-specific high molecular weight proteins in virus-infected BHK cells.

Authors:  G R Cleaves
Journal:  J Gen Virol       Date:  1985-12       Impact factor: 3.891

7.  Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.

Authors:  A L Tarentino; F Maley
Journal:  J Biol Chem       Date:  1974-02-10       Impact factor: 5.157

8.  Change involving a viral membrane glycoprotein during morphogenesis of group B arboviruses.

Authors:  D Shapiro; W E Brandt; P K Russell
Journal:  Virology       Date:  1972-12       Impact factor: 3.616

9.  Dengue haemorrhagic fever--a public health problem and a field for research.

Authors:  S B Halstead
Journal:  Bull World Health Organ       Date:  1980       Impact factor: 9.408

10.  Protection against 17D yellow fever encephalitis in mice by passive transfer of monoclonal antibodies to the nonstructural glycoprotein gp48 and by active immunization with gp48.

Authors:  J J Schlesinger; M W Brandriss; E E Walsh
Journal:  J Immunol       Date:  1985-10       Impact factor: 5.422
View more
  32 in total

1.  Mutational analysis of the octapeptide sequence motif at the NS1-NS2A cleavage junction of dengue type 4 virus.

Authors:  M Pethel; B Falgout; C J Lai
Journal:  J Virol       Date:  1992-12       Impact factor: 5.103

2.  Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2a.

Authors:  B Falgout; R Chanock; C J Lai
Journal:  J Virol       Date:  1989-05       Impact factor: 5.103

3.  Processing of dengue virus type 2 structural proteins containing deletions in hydrophobic domains.

Authors:  A Gruenberg; P J Wright
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

4.  Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses.

Authors:  Y S Hahn; E M Lenches; R Galler; C M Rice; J Dalrymple; J H Strauss
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

5.  Predominance of HLA-restricted cytotoxic T-lymphocyte responses to serotype-cross-reactive epitopes on nonstructural proteins following natural secondary dengue virus infection.

Authors:  A Mathew; I Kurane; S Green; H A Stephens; D W Vaughn; S Kalayanarooj; S Suntayakorn; D Chandanayingyong; F A Ennis; A L Rothman
Journal:  J Virol       Date:  1998-05       Impact factor: 5.103

6.  Cleavage of dengue virus NS1-NS2A requires an octapeptide sequence at the C terminus of NS1.

Authors:  H Hori; C J Lai
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

7.  In vitro processing of dengue virus structural proteins: cleavage of the pre-membrane protein.

Authors:  L Markoff
Journal:  J Virol       Date:  1989-08       Impact factor: 5.103

8.  Dengue virus-specific cross-reactive CD8+ human cytotoxic T lymphocytes.

Authors:  J F Bukowski; I Kurane; C J Lai; M Bray; B Falgout; F A Ennis
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

9.  NS2B-3 proteinase-mediated processing in the yellow fever virus structural region: in vitro and in vivo studies.

Authors:  S M Amberg; A Nestorowicz; D W McCourt; C M Rice
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

10.  Mice immunized with recombinant vaccinia virus expressing dengue 4 virus structural proteins with or without nonstructural protein NS1 are protected against fatal dengue virus encephalitis.

Authors:  M Bray; B T Zhao; L Markoff; K H Eckels; R M Chanock; C J Lai
Journal:  J Virol       Date:  1989-06       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.