| Literature DB >> 33165782 |
Gen Zou1, Zhihua Zhou2.
Abstract
In this protocol, we describe the establishment of a CRISPR/Cas9 system in Trichoderma reesei by generating a specific, codon-optimized Cas9-expressing strain and by in vitro transcription of a gRNA. This system induces mutagenesis or introduces a gene in a targeted way based on PEG-mediated protoplast transformation. Up to three targets, multiplexed genome editing can be obtained in one transformation.Entities:
Keywords: CRISPR/Cas9; Multiplexed genome editing; Protoplast transformation; Specific codon optimization; gRNA transcription
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Year: 2021 PMID： 33165782 DOI： 10.1007/978-1-0716-1048-0_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745