Literature DB >> 3316395

Injection of physiological saline facilitates recovery of ascitic fluids for monoclonal antibody production.

J M Coll1.   

Abstract

The intraperitoneal injection of physiological saline into anti-C-reactive protein (anti-CRP) hybridoma tumor-bearing mice facilitates extraction of diluted ascitic fluids. The dilution with physiological saline before withdrawal of ascitic fluid reduces the processing time in large scale operations, facilitates further manipulations, increases the number of extractions per animal and permits the recovery of ascitic fluid containing monoclonal antibodies from solid tumor-bearing mice.

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Year:  1987        PMID: 3316395     DOI: 10.1016/0022-1759(87)90507-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  3 in total

1.  The free nucleocapsids of the viral haemorrhagic septicaemia virus contain two antigenically related nucleoproteins.

Authors:  B Basurco; F Sanz; M A Marcotegui; J M Coll
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

2.  Antibody-mediated depletion of lymphocyte-activation gene-3 (LAG-3(+) )-activated T lymphocytes prevents delayed-type hypersensitivity in non-human primates.

Authors:  N Poirier; T Haudebourg; C Brignone; N Dilek; J Hervouet; D Minault; F Coulon; R V de Silly; F Triebel; G Blancho; B Vanhove
Journal:  Clin Exp Immunol       Date:  2011-02-24       Impact factor: 4.330

3.  Neutralizing-enhancing monoclonal antibody recognizes the denatured glycoprotein of viral haemorrhagic septicaemia virus.

Authors:  F Sanz; J M Coll
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

  3 in total

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