Literature DB >> 3316194

Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322.

Y Kuriki1.   

Abstract

The amp gene of pBR328 and pBR322 was expressed less efficiently at 45 than at 30 degrees C in an in vitro coupled transcription-translation system of Escherichia coli. Preincubation of S30 extract at 45 degrees C reduced specifically its ability to express the amp gene at 30 degrees C, indicating inactivation of a factor(s) required for efficient expression of the amp gene.

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Year:  1987        PMID： 3316194      PMCID： PMC214187          DOI： 10.1128/jb.169.12.5856-5858.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors:  R A Laskey; A D Mills
Journal:  Eur J Biochem       Date:  1975-08-15

2.  Studies on stringent control in a cell-free system. Regulation by guanosine-5'-diphosphate-3'-diphosphate of the synthesis of elongation factor Tu.

Authors:  M Shibuya; Y Kaziro
Journal:  J Biochem       Date:  1979-08       Impact factor: 3.387

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor.

Authors:  R Landick; V Vaughn; E T Lau; R A VanBogelen; J W Erickson; F C Neidhardt
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

5.  Identification of gene products programmed by restriction endonuclease DNA fragments using an E. coli in vitro system.

Authors:  J M Pratt; G J Boulnois; V Darby; E Orr; E Wahle; I B Holland
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

6.  Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase.

Authors:  W E Taylor; D B Straus; A D Grossman; Z F Burton; C A Gross; R R Burgess
Journal:  Cell       Date:  1984-09       Impact factor: 41.582

7.  The htpR gene product of E. coli is a sigma factor for heat-shock promoters.

Authors:  A D Grossman; J W Erickson; C A Gross
Journal:  Cell       Date:  1984-09       Impact factor: 41.582

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Authors:  X Soberon; L Covarrubias; F Bolivar
Journal:  Gene       Date:  1980-05       Impact factor: 3.688

10.  Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12.

Authors:  T Yamamori; T Yura
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205
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  1 in total

1.  The translation start signal region of TEM beta-lactamase mRNA is responsible for heat shock-induced repression of amp gene expression in Escherichia coli.

Authors:  Y Kuriki
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490
  1 in total

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