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Literature DB >> 3316036

Characterization of the secreted antigens of Mycobacterium bovis BCG: comparison of the 46-kilodalton dimeric protein with proteins MPB64 and MPB70.

Abstract

Western blot analysis showed that the 46-kilodalton (kDa) dimeric protein antigen secreted in large amounts by some daughter strains of Mycobacterium bovis BCG corresponded to protein MPB70 present in long-term culture filtrates of the Japanese substrain. The 46/23-kDa antigen is the most abundant protein in supernatant from a 5-day culture but is masked by leaked products in old culture supernatants. No similarities were found between the 46-kDa protein and MPB64, a protein with the same strain distribution, or with the antigen of similar molecular mass recognized by monoclonal antibody SA1.D2D.

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Year: 1987 PMID： 3316036 PMCID： PMC260053 DOI： 10.1128/iai.55.12.3213-3214.1987

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

7 in total

1. A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles.

Authors: C Abou-Zeid; E Filley; J Steele; G A Rook
Journal: J Immunol Methods Date: 1987-04-02 Impact factor: 2.303

2. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.

Authors: M Harboe; S Nagai; M E Patarroyo; M L Torres; C Ramirez; N Cruz
Journal: Infect Immun Date: 1986-04 Impact factor: 3.441

3. Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG.

Authors: S Nagai; J Matsumoto; T Nagasuga
Journal: Infect Immun Date: 1981-03 Impact factor: 3.441

4. Subdivision of daughter strains of bacille Calmette-Guérin (BCG) according to secreted protein patterns.

Authors: C Abou-Zeid; I Smith; J Grange; J Steele; G Rook
Journal: J Gen Microbiol Date: 1986-11

5. Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae.

Authors: D B Young; M J Fohn; S R Khanolkar; T M Buchanan
Journal: Clin Exp Immunol Date: 1985-06 Impact factor: 4.330

6. MPB70, a unique antigen of Mycobacterium bovis BCG.

Authors: M Harboe; S Nagai
Journal: Am Rev Respir Dis Date: 1984-03

7. Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB70.

Authors: K Miura; S Nagai; M Kinomoto; S Haga; T Tokunaga
Journal: Infect Immun Date: 1983-02 Impact factor: 3.441

7 in total

10 in total

1. Tuberculosis vaccines: current progress.

Authors: Ian M Orme
Journal: Drugs Date: 2005 Impact factor: 9.546

2. Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.

Authors: M Lin; E A Sugden; M E Jolley; K Stilwell
Journal: Clin Diagn Lab Immunol Date: 1996-07

3. Genetic identification of Mycobacterium bovis BCG by restriction fragment length polymorphism analysis of the direct-repeat region.

Authors: S T Howard; A Laszlo; W M Johnson
Journal: J Clin Microbiol Date: 1997-04 Impact factor: 5.948

4. Simple and rapid identification of the Mycobacterium tuberculosis complex by immunochromatographic assay using anti-MPB64 monoclonal antibodies.

Authors: C Abe; K Hirano; T Tomiyama
Journal: J Clin Microbiol Date: 1999-11 Impact factor: 5.948

Characterization of the secreted antigens of Mycobacterium bovis BCG: comparison of the 46-kilodalton dimeric protein with proteins MPB64 and MPB70.

1. A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles.

2. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.

3. Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG.

4. Subdivision of daughter strains of bacille Calmette-Guérin (BCG) according to secreted protein patterns.

5. Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae.

6. MPB70, a unique antigen of Mycobacterium bovis BCG.

7. Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB70.

1. Tuberculosis vaccines: current progress.

2. Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.

3. Genetic identification of Mycobacterium bovis BCG by restriction fragment length polymorphism analysis of the direct-repeat region.

4. Simple and rapid identification of the Mycobacterium tuberculosis complex by immunochromatographic assay using anti-MPB64 monoclonal antibodies.

5. Enzymatic and antigenic analyses of strains of Mycobacterium bovis, M. bovis BCG, and M. tuberculosis.

6. Characterization of fibronectin-binding antigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG.

7. Chemical definition, cloning, and expression of the major protein of the leprosy bacillus.

Review 8. The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis.

9. Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG.

10. Mycobacterium tuberculosis pili (MTP), a putative biomarker for a tuberculosis diagnostic test.