M L Christou1, S Vasileiadis2, S D Kalamaras1, D G Karpouzas2, I Angelidaki3, T A Kotsopoulos4. 1. Department of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece. 2. Department of Biochemistry and Biotechnology, University of Thessaly, GR-41500 Larissa, Greece. 3. Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. 4. Department of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece. Electronic address: mkotsop@agro.auth.gr.
Abstract
Three Continuously Stirred Tank Reactors (CTSRs) were operating at steady state conditions with Organic Loading Rates (OLR) of 2.09, 3.024 and 4.0 g VS L-1 d-1. Glucose was used as the sole factor for increasing the OLR, linking the increase of the OLR with the C/N ratio increase. The reactors were stressed by increasing the ammonia concentration to 5 g L-1 from 1.862 g L-1. The results showed elevating inhibition of the anaerobic process by increasing the C/N ratio just by increasing the OLR, under the high ammonia concentration. A different response of the bacterial and archaeal community under ammonia stressed conditions was also observed. Under the high ammonia concentration, hydrogen-depended methylotrophic was the dominant methanogenesis route at OLR of 2.09 g VS L-1d-1, while the hydrogenotrophic route was the dominant at the high OLR of 4 g VS L-1d-1, which coincided with high acetate and propionate concentrations.
Three Continuously Stirred Tank Reactors (CTSRs) were operating at steady state conditions with Organic Loading Rates (OLR) of 2.09, 3.024 and 4.0 g VS L-1 d-1. Glucose was used as the sole factor for increasing the OLR, linking the increase of the OLR with the C/N ratio increase. The reactors were stressed by increasing the n class="Chemical">ammonia concentration to 5 g L-1 from 1.862 g L-1. The results showed elevating inhibition of the anaerobic process by increasing the C/N ratio just by increasing the OLR, under the high ammonia concentration. A different response of the bacterial and archaeal community under ammonia stressed conditions was also observed. Under the high ammonia concentration, hydrogen-depended methylotrophic was the dominant methanogenesis route at OLR of 2.09 g VS L-1d-1, while the hydrogenotrophic route was the dominant at the high OLR of 4 g VS L-1d-1, which coincided with high acetate and propionate concentrations.
Authors: Marium Saba; Anum Khan; Huma Ali; Amna Bibi; Zeeshan Gul; Alam Khan; Muhammad Maqsood Ur Rehman; Malik Badshah; Fariha Hasan; Aamer Ali Shah; Samiullah Khan Journal: Front Microbiol Date: 2022-04-07 Impact factor: 6.064