Iris Xiaoxue Yin1, Ollie Yiru Yu1, Irene Shuping Zhao2, May Lei Mei3, Quan Li Li4, Jinyao Tang5, Edward Chin Man Lo1, Chun Hung Chu6. 1. Faculty of Dentistry, the University of Hong Kong, Hong Kong, China. 2. School of Stomatology, Shenzhen University Health Science Center, Shenzhen, China. 3. Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, New Zealand. Electronic address: may.mei@otago.ac.nz. 4. College of Stomatology, Anhui Medical University, Hefei, China. 5. Department of Chemistry, the University of Hong Kong, Hong Kong, China. 6. Faculty of Dentistry, the University of Hong Kong, Hong Kong, China. Electronic address: chchu@hku.hk.
Abstract
OBJECTIVES: To investigate the remineralising and staining effects of sodium fluoride (NaF) with silver nanoparticles (AgNPs) on artificial dentine caries. METHODS: Human dentine blocks with artificial caries were divided into four groups. Group 1 received 5 % NaF (22,600 ppm fluoride) with 4000 ppm AgNPs; group 2 received 4000 ppm AgNPs; group 3 received 5 % NaF, group 4 received deionised water (negative control). All groups underwent three biochemical cycles. Each cycle included Streptococcus mutans biofilm challenge and remineralisation process. The lesion depth, mineral-organic content, surface morphology and crystal characteristics of dentine blocks were evaluated using micro-computed tomography, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction. Colour change of dentine blocks was assessed using spectrophotometry. RESULTS: The mean lesion depths of groups 1-4 were 151.13 ± 29.13 μm, 172.38 ± 23.44 μm, 190.41 ± 32.81 μm and 221.24 ± 27.91 μm, respectively. The hydrogen phosphate-to-amide I ratios of groups 1-4 were 5.98 ± 0.36, 3.86 ± 0.56, 4.00 ± 0.67 and 2.53 ± 0.40, respectively. There was no significant interaction effect between AgNPs and NaF. SEM showed less exposure of dentine collagen fibres in group 1 when compared to other groups. X-ray diffraction revealed presence of silver chloride and metallic silver in group 1 and 2. There was no significant difference in colour change among the four groups (p = 0.74). CONCLUSIONS: NaF solution with AgNPs can remineralise dentine caries without staining. CLINICAL SIGNIFICANCE: Sodium fluoride solutions that include silver nanoparticles have potential uses in the management of caries.
OBJECTIVES: To investigate the remineralising and staining effects of sodium fluoride (NaF) with silver nanoparticles (AgNPs) on artificial dentine caries. METHODS:Human dentine blocks with artificial caries were divided into four groups. Group 1 received 5 % NaF (22,600 ppm fluoride) with 4000 ppm AgNPs; group 2 received 4000 ppm AgNPs; group 3 received 5 % NaF, group 4 received deionised water (negative control). All groups underwent three biochemical cycles. Each cycle included Streptococcus mutans biofilm challenge and remineralisation process. The lesion depth, mineral-organic content, surface morphology and crystal characteristics of dentine blocks were evaluated using micro-computed tomography, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction. Colour change of dentine blocks was assessed using spectrophotometry. RESULTS: The mean lesion depths of groups 1-4 were 151.13 ± 29.13 μm, 172.38 ± 23.44 μm, 190.41 ± 32.81 μm and 221.24 ± 27.91 μm, respectively. The hydrogen phosphate-to-amide I ratios of groups 1-4 were 5.98 ± 0.36, 3.86 ± 0.56, 4.00 ± 0.67 and 2.53 ± 0.40, respectively. There was no significant interaction effect between AgNPs and NaF. SEM showed less exposure of dentine collagen fibres in group 1 when compared to other groups. X-ray diffraction revealed presence of silver chloride and metallic silver in group 1 and 2. There was no significant difference in colour change among the four groups (p = 0.74). CONCLUSIONS:NaF solution with AgNPs can remineralise dentine caries without staining. CLINICAL SIGNIFICANCE: Sodium fluoride solutions that include silver nanoparticles have potential uses in the management of caries.
Authors: John Yun Niu; Iris Xiaoxue Yin; William Ka Kei Wu; Quan-Li Li; May Lei Mei; Chun Hung Chu Journal: Clin Oral Investig Date: 2021-10-12 Impact factor: 3.573