Literature DB >> 3315657

A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis.

G L Erexson1, A D Kligerman.   

Abstract

A detailed methodology is presented for culturing mouse peripheral blood lymphocytes isolated on density gradients and stimulated to divide using either phytohemagglutinin, concanavalin A, or lipopolysaccharide. The techniques described yield more than sufficient numbers of mitotic cells for analyzing sister chromatid exchange, chromosome aberrations, and micronuclei following in vitro or in vivo exposure to chemicals or radiation.

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Year:  1987        PMID： 3315657     DOI： 10.1002/em.2850100407

Source DB:  PubMed          Journal:  Environ Mol Mutagen        ISSN： 0893-6692            Impact factor:   3.216


  10 in total

1.  A mouse model of cytogenetic analysis to evaluate caesium137 radiation dose exposure and contamination level in lymphocytes.

Authors:  Sandrine Roch-Lefèvre; Cécile Martin-Bodiot; Eric Grégoire; Aurélie Desbrée; Laurence Roy; Joan Francesc Barquinero
Journal:  Radiat Environ Biophys       Date:  2016-01-18       Impact factor: 1.925

2.  Reciprocal translocations in somatic and germ cells of mice chronically exposed by inhalation to ethylene oxide: implications for risk assessment.

Authors:  E Maria Donner; Brian A Wong; R Arden James; R Julian Preston
Journal:  Mutagenesis       Date:  2009-11-01       Impact factor: 3.000

3.  The cytogenetic evaluation of in vivo genotoxic and cytotoxic activity using rodent somatic cells.

Authors:  R R Tice
Journal:  Cell Biol Toxicol       Date:  1988-12       Impact factor: 6.691

4.  Oxidation and methylation status determine the effects of arsenic on the mitotic apparatus.

Authors:  A D Kligerman; C L Doerr; A H Tennant
Journal:  Mol Cell Biochem       Date:  2005-11       Impact factor: 3.396

5.  Effect of dose rate on residual γ-H2AX levels and frequency of micronuclei in X-irradiated mouse lymphocytes.

Authors:  H C Turner; I Shuryak; M Taveras; A Bertucci; J R Perrier; C Chen; C D Elliston; G W Johnson; L B Smilenov; S A Amundson; D J Brenner
Journal:  Radiat Res       Date:  2015-03-04       Impact factor: 2.841

6.  Cytogenetic Damage of Human Lymphocytes in Humanized Mice Exposed to Neutrons and X Rays 24 h After Exposure.

Authors:  Qi Wang; Younghyun Lee; Monica Pujol-Canadell; Jay R Perrier; Lubomir Smilenov; Andrew Harken; Guy Garty; David J Brenner; Brian Ponnaiya; Helen C Turner
Journal:  Cytogenet Genome Res       Date:  2021-09-06       Impact factor: 1.941

7.  Detection of chromosome malsegregation to the daughter nuclei in cytokinesis-blocked transgenic mouse splenocytes.

Authors:  J J Boei; A T Natarajan
Journal:  Chromosome Res       Date:  1995-01       Impact factor: 5.239

8.  Effects on the immune system associated with living near a pesticide dump site.

Authors:  M F Vine; L Stein; K Weigle; J Schroeder; D Degnan; C K Tse; C Hanchette; L Backer
Journal:  Environ Health Perspect       Date:  2000-12       Impact factor: 9.031

9.  Induction of Genomic Instability in a Primary Human Fibroblast Cell Line Following Low-Dose Alpha-Particle Exposure and the Potential Role of Exosomes.

Authors:  Eman Mohammed Elbakrawy; Ammar Mayah; Mark A Hill; Munira Kadhim
Journal:  Biology (Basel)       Date:  2020-12-28

10.  Radiation-induced Chromosome Instability: The Role of Dose and Dose Rate.

Authors:  Eman Mohammed Elbakrawy; Mark A Hill; Munira A Kadhim
Journal:  Genome Integr       Date:  2019-10-25
  10 in total

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