Suhair M Jabbar1, Nihad A M Al-Rashedi2. 1. Department of Biology, College of Science, Al Muthanna University, Samawah, Iraq. 2. Department of Biology, College of Science, Al Muthanna University, Samawah, Iraq. nhidaee@mu.edu.iq.
Abstract
BACKGROUND: Although Iraq has one of the world's oldest cultural histories and an important geographic location, forensic reference data on mitochondrial DNA (mtDNA) control region in Iraqi populations are scarce, particularly for populations residing in the southern part of Iraq. Mitochondrial DNA typing is an excellent tool for forensic investigations and in missing-person cases because of its unique qualities, such as mtDNA non-coding control region with specific genetic markers, high copy numbers in cells, maternal inheritance, and lack of recombination. METHODS: Forensic analysis was performed on the entire mtDNA control region in 203 unrelated Iraqi individuals residing in Samawah City of Iraq. Polymorphisms in the mtDNA were detected using polymerase chain reaction and Sanger-type sequencing, and the sequences were aligned to compare with revised Cambridge Reference Sequence (rCRS). RESULTS: The sequencing results revealed 111 haplotypes characterized by 143 polymorphic positions. Of these haplotypes, 63 were unique and 48 were shared by more than one person. The haplotype data generated in this study will be available on EMPOP via accession number EMP00814.
BACKGROUND: Although Iraq has one of the world's oldest cultural histories and an important geographic location, forensic reference data on mitochondrial DNA (mtDNA) control region in Iraqi populations are scarce, particularly for populations residing in the southern part of Iraq. Mitochondrial DNA typing is an excellent tool for forensic investigations and in missing-person cases because of its unique qualities, such as mtDNA non-coding control region with specific genetic markers, high copy numbers in cells, maternal inheritance, and lack of recombination. METHODS: Forensic analysis was performed on the entire mtDNA control region in 203 unrelated Iraqi individuals residing in Samawah City of Iraq. Polymorphisms in the mtDNA were detected using polymerase chain reaction and Sanger-type sequencing, and the sequences were aligned to compare with revised Cambridge Reference Sequence (rCRS). RESULTS: The sequencing results revealed 111 haplotypes characterized by 143 polymorphic positions. Of these haplotypes, 63 were unique and 48 were shared by more than one person. The haplotype data generated in this study will be available on EMPOP via accession number EMP00814.
Keywords:
EMPOP; Forensic population data; Forensic science; Haplogroups; Iraq; mtDNA
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