Elizabeth Madla-Cruz1, Myriam De la Garza-Ramos2, César I Romo-Sáenz1, Patricia Tamez-Guerra1, Marco A Garza-Navarro3, Victor Urrutia-Baca4, María A Martínez-Rodríguez3, Ricardo Gomez-Flores5. 1. Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolas de los Garza, Nuevo León, C.P. 66455, Mexico. 2. Universidad Autonoma de Nuevo Leon, Facultad de Odontología/Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Monterrey, Nuevo León, CP. 64460, Mexico. 3. Universidad Autonoma de Nuevo Leon, Facultad de Ingeniería Mecánica y Eléctrica, San Nicolás de los Garza, Nuevo León, C.P. 66455, Mexico. 4. CHRISTUS Excellence and Innovation Center, Monterrey, N.L., C.P. 66260, Mexico. 5. Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolas de los Garza, Nuevo León, C.P. 66455, Mexico. Electronic address: ricardo.gomezf@uanl.mx.
Abstract
OBJECTIVE: To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms. DESIGN: AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay. In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms. RESULTS: Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized. They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL. In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control. We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs. CONCLUSION: AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
OBJECTIVE: To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms. DESIGN:AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay. In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms. RESULTS: Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized. They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL. In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control. We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs. CONCLUSION:AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.