Literature DB >> 33145715

3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.

Mariana A Branco1, Tiago P Dias1, João P Cotovio1, Carlos A V Rodrigues1, Tiago G Fernandes1, Joaquim M S Cabral1, Maria Margarida Diogo2.   

Abstract

The generation of cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs) represents a valuable tool for a myriad of in vitro applications, including drug screening, disease modeling and regenerative medicine. However, the success of these applications is dependent on the establishment of reliable, efficient, simple, and cost-effective differentiation methods. In this chapter, we describe an efficient and robust 3D platform for the generation of hPSC-CMs based on the use of a microwell culture system, which can be applied in any laboratory environment. Additionally, we will also describe protocols for the structural and functional characterization of the obtained CMs for further quality control upon differentiation.
© 2020. Springer Science+Business Media New York.

Entities:  

Keywords:  3D aggregates; Cardiac differentiation; Cardiomyocytes; Human pluripotent stem cells; Microwells

Mesh：

Year:  2022        PMID： 33145715     DOI： 10.1007/7651_2020_336

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

1.  Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells.

Authors:  Vincent C Chen; Jingjing Ye; Praveen Shukla; Giau Hua; Danlin Chen; Ziguang Lin; Jian-chang Liu; Jing Chai; Joseph Gold; Joseph Wu; David Hsu; Larry A Couture
Journal:  Stem Cell Res       Date:  2015-08-13       Impact factor: 2.020

2.  Universal cardiac induction of human pluripotent stem cells in two and three-dimensional formats: implications for in vitro maturation.

Authors:  Miao Zhang; Jan Sebastian Schulte; Alexander Heinick; Ilaria Piccini; Jyoti Rao; Roberto Quaranta; Dagmar Zeuschner; Daniela Malan; Kee-Pyo Kim; Albrecht Röpke; Philipp Sasse; Marcos Araúzo-Bravo; Guiscard Seebohm; Hans Schöler; Larissa Fabritz; Paulus Kirchhof; Frank Ulrich Müller; Boris Greber
Journal:  Stem Cells       Date:  2015-05       Impact factor: 6.277

3.  The use of agarose microwells for scalable embryoid body formation and cardiac differentiation of human and murine pluripotent stem cells.

Authors:  Julia Dahlmann; George Kensah; Henning Kempf; David Skvorc; Anke Gawol; David A Elliott; Gerald Dräger; Robert Zweigerdt; Ulrich Martin; Ina Gruh
Journal:  Biomaterials       Date:  2013-01-16       Impact factor: 12.479

4.  A Universal and Robust Integrated Platform for the Scalable Production of Human Cardiomyocytes From Pluripotent Stem Cells.

Authors:  Hananeh Fonoudi; Hassan Ansari; Saeed Abbasalizadeh; Mehran Rezaei Larijani; Sahar Kiani; Shiva Hashemizadeh; Ali Sharifi Zarchi; Alexis Bosman; Gillian M Blue; Sara Pahlavan; Matthew Perry; Yishay Orr; Yaroslav Mayorchak; Jamie Vandenberg; Mahmood Talkhabi; David S Winlaw; Richard P Harvey; Nasser Aghdami; Hossein Baharvand
Journal:  Stem Cells Transl Med       Date:  2015-10-28       Impact factor: 6.940

5.  An intermittent rocking platform for integrated expansion and differentiation of human pluripotent stem cells to cardiomyocytes in suspended microcarrier cultures.

Authors:  Sherwin Ting; Allen Chen; Shaul Reuveny; Steve Oh
Journal:  Stem Cell Res       Date:  2014-06-27       Impact factor: 2.020

6.  A massive suspension culture system with metabolic purification for human pluripotent stem cell-derived cardiomyocytes.

Authors:  Natsuko Hemmi; Shugo Tohyama; Kazuaki Nakajima; Hideaki Kanazawa; Tomoyuki Suzuki; Fumiyuki Hattori; Tomohisa Seki; Yoshikazu Kishino; Akinori Hirano; Marina Okada; Ryota Tabei; Rei Ohno; Chihana Fujita; Tomoko Haruna; Shinsuke Yuasa; Motoaki Sano; Jun Fujita; Keiichi Fukuda
Journal:  Stem Cells Transl Med       Date:  2014-10-29       Impact factor: 6.940

7.  A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability.

Authors:  Paul W Burridge; Susan Thompson; Michal A Millrod; Seth Weinberg; Xuan Yuan; Ann Peters; Vasiliki Mahairaki; Vassilis E Koliatsos; Leslie Tung; Elias T Zambidis
Journal:  PLoS One       Date:  2011-04-08       Impact factor: 3.240

8.  Self-organizing human cardiac microchambers mediated by geometric confinement.

Authors:  Zhen Ma; Jason Wang; Peter Loskill; Nathaniel Huebsch; Sangmo Koo; Felicia L Svedlund; Natalie C Marks; Ethan W Hua; Costas P Grigoropoulos; Bruce R Conklin; Kevin E Healy
Journal:  Nat Commun       Date:  2015-07-14       Impact factor: 14.919

9.  Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture.

Authors:  Henning Kempf; Ruth Olmer; Christina Kropp; Michael Rückert; Monica Jara-Avaca; Diana Robles-Diaz; Annika Franke; David A Elliott; Daniel Wojciechowski; Martin Fischer; Angelica Roa Lara; George Kensah; Ina Gruh; Axel Haverich; Ulrich Martin; Robert Zweigerdt
Journal:  Stem Cell Reports       Date:  2014-10-30       Impact factor: 7.765

10.  Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.

Authors:  Caroline Halloin; Kristin Schwanke; Wiebke Löbel; Annika Franke; Monika Szepes; Santoshi Biswanath; Stephanie Wunderlich; Sylvia Merkert; Natalie Weber; Felix Osten; Jeanne de la Roche; Felix Polten; Kai Christoph Wollert; Theresia Kraft; Martin Fischer; Ulrich Martin; Ina Gruh; Henning Kempf; Robert Zweigerdt
Journal:  Stem Cell Reports       Date:  2019-07-25       Impact factor: 7.765
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