Christina Hadjichristou1, Imad About2, Petros Koidis3, Athina Bakopoulou3. 1. Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki (A.U.Th), GR-54124, Thessaloniki, Greece. cchatzic@dent.auth.gr. 2. Centre National de la Recherche Scientifique, Institute of Movement Sciences, Aix Marseille University, Marseille, France. 3. Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki (A.U.Th), GR-54124, Thessaloniki, Greece.
Abstract
OBJECTIVE: Experimental procedures have been used to monitor cellular responses at the dentin/pulp interface. Aiming to divert from in vivo studies and oversimplified two-dimensional assays, three-dimensional (3D) models have been developed. This review provides an overview of existing literature, regarding 3D in vitro dentin/pulp reconstruction. MATERIAL & METHODS: PubMed, Scopus, Cochrane Library and Web of Science- were systematically searched for attributes between 1998 and 2020. The search focused on articles on the development of three-dimensional tools for the reconstruction of a dentin/pulp complex under in vitro conditions, which were then screened and qualitatively assessed. Article grouping according to mode of implementation, resulted in five categories: the customised cell perfusion chamber (CPC) (n = 8), the tooth bud model (TBM) (n = 3), the 3D dentin/pulp complex manufactured by tissue engineering (DPC) (n = 6), the entire tooth culture (ETC) (n = 4) and the tooth slice culture model (TSC) (n = 5). RESULTS: A total of 26 publications, applying nine and eight substances for pulp and dentin representation respectively, were included. Natural materials and dentin components were the most widely utilized. The most diverse category was the DPC, while the CPC group was the test with the highest longevity. The most consistent categories were the ETC and TSC models, while the TBM presented as the most complete de novo approach. CONCLUSIONS: All studies presented with experimental protocols with potential upgrades. Solving the limitations of each category will provide a complete in vitro testing and monitoring tool of dental responses to exogenous inputs. CLINICAL RELEVANCE: The 3D dentin/pulp complexes are valid supplementary tools for in vivo studies and clinical testing. Graphical Abstract.
OBJECTIVE: Experimental procedures have been used to monitor cellular responses at the dentin/pulp interface. Aiming to divert from in vivo studies and oversimplified two-dimensional assays, three-dimensional (3D) models have been developed. This review provides an overview of existing literature, regarding 3D in vitro dentin/pulp reconstruction. MATERIAL & METHODS: PubMed, Scopus, Cochrane Library and Web of Science- were systematically searched for attributes between 1998 and 2020. The search focused on articles on the development of three-dimensional tools for the reconstruction of a dentin/pulp complex under in vitro conditions, which were then screened and qualitatively assessed. Article grouping according to mode of implementation, resulted in five categories: the customised cell perfusion chamber (CPC) (n = 8), the tooth bud model (TBM) (n = 3), the 3D dentin/pulp complex manufactured by tissue engineering (DPC) (n = 6), the entire tooth culture (ETC) (n = 4) and the tooth slice culture model (TSC) (n = 5). RESULTS: A total of 26 publications, applying nine and eight substances for pulp and dentin representation respectively, were included. Natural materials and dentin components were the most widely utilized. The most diverse category was the DPC, while the CPC group was the test with the highest longevity. The most consistent categories were the ETC and TSC models, while the TBM presented as the most complete de novo approach. CONCLUSIONS: All studies presented with experimental protocols with potential upgrades. Solving the limitations of each category will provide a complete in vitro testing and monitoring tool of dental responses to exogenous inputs. CLINICAL RELEVANCE: The 3D dentin/pulp complexes are valid supplementary tools for in vivo studies and clinical testing. Graphical Abstract.
Authors: Kathleen A Fitzgerald; Meenakshi Malhotra; Caroline M Curtin; Fergal J O' Brien; Caitriona M O' Driscoll Journal: J Control Release Date: 2015-07-26 Impact factor: 9.776
Authors: Kirsten L Van Landuyt; Stephanie Krifka; Karl-Anton Hiller; Carola Bolay; Claudia Waha; Bart Van Meerbeek; Gottfried Schmalz; Helmut Schweikl Journal: Dent Mater Date: 2015-05-27 Impact factor: 5.304
Authors: Mauricio Gonçalves da Costa Sousa; Gabriela Conceição de Almeida; Danilo César Martins Mota; Rosiane Andrade da Costa; Simoni Campos Dias; Samuel Nunes Limberger; Frank Ko; Li Ting Lin; Evan F Haney; Hashem Etayash; Beverlie Baquir; Michael J Trimble; Ya Shen; Zheng Su; Markus Haapasalo; Daniel Pletzer; Letícia Chaves de Souza; Gláucia Schuindt Teixeira; Renato M Silva; Robert E W Hancock; Octavio Luiz Franco; Taia Maria Berto Rezende Journal: Bioact Mater Date: 2022-02-01