Takuya Murata1, Chihiro Hozumi2, Yukihiko Hiroshima3, Koichiro Shimoya4, Atsushi Hongo5, Sachiko Inubushi6, Hirokazu Tanino6, Robert M Hoffman7,8. 1. Department of Obstetrics and Gynecology 2, Kawasaki Medical School, Okayama, Japan t-muratactac@gmail.com all@anticancer.com. 2. AntiCancer Japan Inc, Narita, Japan. 3. Department of Cancer Genome Medicine, Kanagawa Cancer Center Research Institute, Kanagawa, Japan. 4. Department of Obstetrics and Gynecology 1, Kawasaki Medical School, Kurashiki, Japan. 5. Department of Obstetrics and Gynecology 2, Kawasaki Medical School, Okayama, Japan. 6. Breast Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. 7. AntiCancer Inc, San Diego, CA, U.S.A. t-muratactac@gmail.com all@anticancer.com. 8. Department of Surgery, University of California, San Diego, CA, U.S.A.
Abstract
BACKGROUND/AIM: The discovery of the nude mouse model enabled the experimental growth of human-patient tumors. However, the low establishment rate of tumors in nude and other immunodeficient strains of mice has limited wide-spread clinical use. MATERIALS AND METHODS: In order to increase the establishment rate of surgical specimens of patient tumors, we transplanted tumors to nude mice subcutaneously along with large amounts of surrounding tissue of the tumor. RESULTS: The new transplantation method increased the establishment rate in nude mice to 66% compared to the old method of implanting the surgical tumor specimen with surrounding tissue removed (14%). High stage and presence of metastasis in the patient donor are positively correlated to tumor engraftment in nude mice. CONCLUSION: The new method can potentially allow most cancer patients who undergo surgery or biopsy to have their own mouse model for drug-sensitivity testing. Copyright
BACKGROUND/AIM: The discovery of the nude mouse model enabled the experimental growth of human-patienttumors. However, the low establishment rate of tumors in nude and other immunodeficient strains of mice has limited wide-spread clinical use. MATERIALS AND METHODS: In order to increase the establishment rate of surgical specimens of patienttumors, we transplanted tumors to nude mice subcutaneously along with large amounts of surrounding tissue of the tumor. RESULTS: The new transplantation method increased the establishment rate in nude mice to 66% compared to the old method of implanting the surgical tumor specimen with surrounding tissue removed (14%). High stage and presence of metastasis in the patient donor are positively correlated to tumor engraftment in nude mice. CONCLUSION: The new method can potentially allow most cancerpatients who undergo surgery or biopsy to have their own mouse model for drug-sensitivity testing. Copyright
Authors: Eunsung Jun; Yejong Park; Woohyung Lee; Jaewoo Kwon; Song Lee; Moon Bo Kim; Ji Sun Lee; Ki Byung Song; Dae Wook Hwang; Jae Hoon Lee; Robert M Hoffman; Song Cheol Kim Journal: Sci Rep Date: 2020-07-20 Impact factor: 4.379
Authors: Noriyuki Masaki; Yutaka Yonemura; Nathaniel F Wu; Carissa Samonte; Chihiro Hozumi; Yutaro Kubota; Yusuke Aoki; Michael Bouvet; Jun Miyazaki; Robert M Hoffman Journal: In Vivo Date: 2022 Jul-Aug Impact factor: 2.406