BACKGROUND/AIM: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant human cancer. MATERIALS AND METHODS: A highly-fluorescent green fluorescent protein (GFP)-expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 human bladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box. RESULTS: The GFP-expressing orthotopic bladder tumor was imaged in real-time non-invasively as well as intra-vitally, with the two methods correlating at r=0.99. CONCLUSION: This is the first non-invasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease. Copyright
BACKGROUND/AIM: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant humancancer. MATERIALS AND METHODS: A highly-fluorescent green fluorescent protein (GFP)-expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 humanbladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box. RESULTS: The GFP-expressing orthotopic bladder tumor was imaged in real-time non-invasively as well as intra-vitally, with the two methods correlating at r=0.99. CONCLUSION: This is the first non-invasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease. Copyright
Authors: Sarah E Lee; Shawn F Bairstow; Jane O Werling; Mahesh V Chaubal; Lawrence Lin; Mary Ann Murphy; James P DiOrio; Jerome Gass; Barrett Rabinow; Xiaoen Wang; Yong Zhang; Zhijian Yang; Robert M Hoffman Journal: Pharm Dev Technol Date: 2013-04-25 Impact factor: 3.133
Authors: T H Kuo; T Kubota; M Watanabe; T Furukawa; S Kase; H Tanino; Y Saikawa; K Ishibiki; M Kitajima; R M Hoffman Journal: Anticancer Res Date: 1993 May-Jun Impact factor: 2.480
Authors: Matthew H Katz; Shinako Takimoto; Daniel Spivack; A R Moossa; Robert M Hoffman; Michael Bouvet Journal: J Surg Res Date: 2003-07 Impact factor: 2.192
Authors: Fang-Xian Sun; Akiko Tohgo; Michael Bouvet; Shigeo Yagi; Rounak Nassirpour; Abdoul R Moossa; Robert M Hoffman Journal: Cancer Res Date: 2003-01-01 Impact factor: 12.701