Sexual Dimorphism in the Chemical Composition of Male and Female in the Dioecious Tree, Juniperus communis L., Growing under Different Nutritional Conditions.

We hypothesized that female and male individuals of the dioecious tree species, Juniperus communis, exhibit different strategies of resource allocation when growing under stress conditions. To test this hypothesis, we performed a two-year pot experiment on plants exposed to different levels of nutrient availability. Analysis of the plants revealed a higher concentration of carbohydrates, carbon, and phenolic compounds in needles of female plants, indicating that females allocate more resources to storage and defense than males. This difference was independent of nutrient availability. Differences in carbohydrates levels between the sexes were most often significant in June, during the most intensive phase of vegetative growth in both sexes, but could also be attributed to female resources investment in cone development. A higher level of nitrogen and other macroelements was observed in males than in females, which may have been connected to the accumulation of resources (nitrogen) for pollen grain production in males or greater allocation of these elements to seeds and cones in females. The interaction between sex and soil fertilization for the C:N ratio may also indicate sex-specific patterns of resource allocation and utilization, which is impacted by their availability during specific periods of J. communis annual life cycle.

1. Introduction

Females of dioecious plants are often reported to show a greater reproductive effort than male plants [1,2] and this effort is more resource-dependent [3]. Females produce resource-consuming seeds and fruits (or cones) that carry substantial cost and for this reason, their reproductive effort can be greater than in males, despite the fact that males often produce a greater number of flowers (e.g., [4]). n class="Species">Juniperus communis L., a dioecious tree or shrub commoclass="Chemical">n iclass="Chemical">n the class="Chemical">northerclass="Chemical">n hemisphere, is aclass="Chemical">n example of a species that exhibits a greater reproductive effort iclass="Chemical">n females thaclass="Chemical">n iclass="Chemical">n males [5]. Due to that, females iclass="Chemical">n this species are thought to be more proclass="Chemical">ne to suffer from eclass="Chemical">nviroclass="Chemical">nmeclass="Chemical">ntal class="Chemical">n class="Disease">stress than males. Other studies show that in dioecious species multiple stresses impact female plants more severely than male plants [6]. In this regard, females are more susceptible inter alia to drought [6,7,8], herbivory [6], and in some dioecious species, they are also affected by nutritional limitations [9].

The differences in n class="Disease">stress resistaclass="Chemical">nce observed betweeclass="Chemical">n male aclass="Chemical">nd female placlass="Chemical">nts caclass="Chemical">n result iclass="Chemical">n greater female class="Chemical">n class="Disease">mortality [7]. This, however, is attributed also to the greater reproductive effort of females [10,11]. As a consequence, this can lead to a male-biased sex ratio. Lower stress tolerance in females has also been reported to influence the geographical range of populations [12].

The different reproductive roles of male and female individuals not only lead to the generation of different reproductive structures, but they are also connected with the secondary sexual dimorphism, i.e., not directly connected with generative organs. It can be observed inter alia as different resource demands. For example, male reproductive structures are reported to require more n class="Chemical">nitrogen for polleclass="Chemical">n productioclass="Chemical">n while females require more class="Chemical">n class="Chemical">carbon for seed development [13]. In this regard, greater concentrations of carbohydrates have been reported in females by many authors (e.g., [14,15]). Moreover, different concentrations of secondary metabolites have also been reported in some dioecious woody plants [16].

In addition to inherent differences in resource composition in males and females, resource allocation can vary between both sexes as the response of females to greater reproductive costs [17], but also as the response and adaptation to local environmental conditions [18]. In a longer time perspective, resource allocation to reproduction in females can be greater even if males flower earlier and more frequently than females [17]. As a consequence, males can use a greater amount of resources available for vegetative growth than females [17]. In regards to n class="Species">J. communis, however, it is importaclass="Chemical">nt to class="Chemical">note that female coclass="Chemical">nes are able to photosyclass="Chemical">nthesize as it was observed iclass="Chemical">n class="Chemical">n class="Species">Taxus baccata L. green arils [19] and they compensate at least a part of their production costs [20].

n class="Disease">Stress coclass="Chemical">nditioclass="Chemical">ns (e.g., drought, class="Chemical">nutrieclass="Chemical">nt deficieclass="Chemical">ncy) caclass="Chemical">n affect allocatioclass="Chemical">n strategies differeclass="Chemical">ntly iclass="Chemical">n males aclass="Chemical">nd females [21]. The depletioclass="Chemical">n of class="Chemical">nutrieclass="Chemical">nts stored iclass="Chemical">n stems caclass="Chemical">n have a more severe effect oclass="Chemical">n reproductioclass="Chemical">n iclass="Chemical">n females thaclass="Chemical">n iclass="Chemical">n males [9]. Iclass="Chemical">n fact, differeclass="Chemical">nces iclass="Chemical">n the sex-related allocatioclass="Chemical">n of resources iclass="Chemical">n dioecious species growiclass="Chemical">ng uclass="Chemical">nder differeclass="Chemical">nt class="Chemical">nutritioclass="Chemical">nal coclass="Chemical">nditioclass="Chemical">ns have beeclass="Chemical">n reported [14,22]. Despite some research coclass="Chemical">ncerclass="Chemical">niclass="Chemical">ng differeclass="Chemical">nces betweeclass="Chemical">n sexes, further studies are class="Chemical">needed pertaiclass="Chemical">niclass="Chemical">ng to life-history traits aclass="Chemical">nd resource allocatioclass="Chemical">n strategies [23].

Most of the nutrients obtained by plants originate in the soil and soil fertilization influences the overall physiology of a plant. n class="Chemical">Nitrogen aclass="Chemical">nd class="Chemical">n class="Chemical">phosphorus are the most limiting nutrients in plants [24]. Nitrogen deficiency has been demonstrated to affect the expression of genes connected with N and carbohydrate metabolism, secondary metabolism, and stress response [25]. Moreover, higher nitrogen availability increases the uptake of phosphorus [26].

Seed and fruit (or female cone) formation requires a significant amount of macro- and microelements. One of the most abundant elements in seeds are P and Ca, while K is present to a lesser extent [27]. Seed and accompanied structure formation requires also additional resources. Their reservoirs in plants are mainly n class="Chemical">starch aclass="Chemical">nd soluble class="Chemical">n class="Chemical">sugars. Starch synthesis serves as a method of storing energy in plants, while soluble sugars are typically used for ongoing metabolism [28]. A higher concentration of soluble sugars can be achieved by starch hydrolysis, while this can be induced in response to the energy needs for other physiological processes [29]. Moreover, stress can result in lower starch levels [30].

In conclusion, dioecious plants often show secondary sexual dimorphism and the sexes differ in reproductive effort, resource demands, and allocation as well as in their response to n class="Disease">stress. We feel that studies about the iclass="Chemical">nflueclass="Chemical">nce of loclass="Chemical">ng-term class="Chemical">nutritioclass="Chemical">n limitatioclass="Chemical">n oclass="Chemical">n resource storage strategies iclass="Chemical">n both sexes are class="Chemical">needed, especially regardiclass="Chemical">ng particular class="Chemical">n class="Chemical">phenological phases. Previous studies showed that Juniperus communis is a good model species for the study of sexual dimorphism in dioecious plants. Significant differences in growth rate, morphology, and ecophysiology were found in this species [5,31,32], but no detailed analysis of chemical composition, considering both differences between sexes and soil fertilization treatment, have been conducted. Moreover, similarly to many other dioecious species, a decline in population size of J. communis and a sex ratio bias are observed [33,34,35,36] which makes such studies even more necessary.

In the current study, we attempted to determine if male and female plants of n class="Species">J. communis differ iclass="Chemical">n their respoclass="Chemical">nse to loclass="Chemical">ng-term class="Chemical">nutrieclass="Chemical">nt limitatioclass="Chemical">ns iclass="Chemical">n regards to the syclass="Chemical">nthesis aclass="Chemical">nd allocatioclass="Chemical">n of class="Chemical">n class="Chemical">carbohydrates, macroelements, and phenolic compounds in leaves. At the same time, we focused on this response during the annual cycle of growth. We hypothesized that:

Females allocate more resources to storage and defense than males. Therefore, female leaves have a greater concentration of class="Chemical">carbon, class="Chemical">n class="Chemical">carbohydrates, and C:N ratio, as well as phenolic compounds, but a lower concentration of other macroelements.

Differences between sexes are most evident when plants are grown under nutrient limited conditions.

Differences between male and female plants will be most pronounced after strobili opening when females produce seeds and accompanying structures and during the most intensive period of vegetative growth. Differences would also be evident in winter (December) when additional n class="Disease">stresses, such as low temperatures, occur.

2. Results

2.1. Effect of Soil Fertilization

n class="Chemical">No differeclass="Chemical">nces iclass="Chemical">n the coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n of soluble class="Chemical">n class="Chemical">sugars were observed between fertilized (F) and nonfertilized (NF) plants. Starch and total nonstructural carbohydrates (TNC) concentrations, however, were higher in NF plants in March during both years of the study (2014 starch: NF 5.06 ± 0.61, F 1.75 ± 0.19, p < 0.0001; 2014 TNC: NF 13.36 ± 0.70, F 10.23 ± 0.21, p = 0.0002; 2015 starch: NF 5.35 ± 0.57, F 2.61 ± 0.38, p = 0.0011; 2015 TNC: NF 12.66 ± 1.08, F 8.99 ± 0.69, p = 0.0099) and in June 2014 (starch: NF 7.66 ± 0.97, F 5.32 ± 0.67, p = 0.0234; TNC: NF 13.13 ± 0.99, F 10.76 ± 0.72, p = 0.0156). The concentration of total phenolic compounds was higher in NF plants in March 2015 (NF 100.73 ± 4.14, F 80.44 ± 5.78, p = 0.0074) and December 2015 (NF 198.85 ± 5.65, F 177.98 ± 5.39, p = 0.0061) (Tables S1 and S2).

Fertilized plants had a higher concentration of C in March of both years (2014 n class="Chemical">NF 47.39 ± 0.12, F 47.94 ± 0.09, p = 0.0019; 2015 class="Chemical">n class="Chemical">NF 47.65 ± 0.15, F 48.44 ± 0.16, p = 0.0008) and in September 2014 (NF 48.43 ± 0.15, F 48.85 ± 0.16, p = 0.0309). They also had a higher concentration of C in June 2015 (NF 47.52 ± 0.09, F 47.83 ± 0.07, p = 0.013). N concentration and the C:N ratio were both significantly different in plants growing under different nutritional conditions over the entire course of the study. Notably, the concentration of N was higher in F plants, and the C:N ratio was higher in NF plants.

Fertilized plants had a significantly higher concentration of P than n class="Chemical">NF placlass="Chemical">nts duriclass="Chemical">ng four sampliclass="Chemical">ng times (March 2014 class="Chemical">n class="Chemical">NF 0.18 ± 0.01, F 0.24 ± 0.01, p = 0.0019; July 2014 NF 0.14 ± 0.01, F 0.16 ± 0.01, p = 0.0134; December 2014 NF 0.18 ± 0.01, F 0.25 ± 0.01, p < 0.0001; March 2015 NF 0.16 ± 0.01, F 0.26 ± 0.01, p < 0.0001), while the results were reversed in September 2015 (NF 0.22 ± 0.01, F 0.21 ± 0.01, p = 0.0462). Differences in the concentration of K varied, with F plants having a higher concentration in September 2014 (NF 0.80 ± 0.02, F 0.90 ± 0.04, p = 0.0020) but a lower concentration in December 2014 (NF 0.67 ± 0.02, F 0.57 ± 0.02, p = 0.0012), followed by a higher concentration in F plants again in March 2015 (NF 0.64 ± 0.02, F 0.78 ± 0.02, p = 0.0002), and then a higher concentration in NF plants in July 2015 (NF 0.76 ± 0.02, F 0.68 ± 0.02, p = 0.0098). The concentration of Ca was higher in F plants over the course of the entire study, however, the concentration of Mg was significantly higher in F plants only in March 2015 (NF 0.18 ± 0.01, F 0.20 ± 0.01, p = 0.0255) (Table S2).

2.2. Effects of Sex and Sex × Soil Fertilization Interactions

Female individuals exhibited a higher concentration of soluble n class="Chemical">sugars, class="Chemical">n class="Chemical">starch, TNC, and total phenolic compounds (TPhC) than male individuals (Figure 1, Table S1). The concentration of soluble sugars in females, however, was only higher in July 2015 (♀ 7.11 ± 0.27, ♂ 6.19 ± 0.24, p = 0.0197) (Figure 1a, Table S2). At this sampling time point (July 2015), the concentration of starch and TNC was also higher in female plants than in male plants (starch: ♀ 3.91 ± 0.85, ♂ 1.43 ± 0.36, p = 0.0103; TNC: ♀ 11.02 ± 0.82, ♂ 7.80 ± 0.43, p = 0.0033). Those differences were also evident in July 2014 (starch: ♀ 8.28 ± 0.99, ♂ 4.91 ± 0.45, p = 0.0031; TNC: ♀ 13.25 ± 1.00, ♂ 10.66 ± 0.68, p = 0.0107) (Figure 1b,c). The concentration of starch was also higher in females than in males in March 2014 (♀ 3.95 ± 0.73, ♂ 2.56 ± 0.52, p = 0.0072) and December 2014 (♀ 0.46 ± 0.00, ♂ 0.44 ± 0.00, p = 0.0465) (Figure 1b). The concentration of TPhC was higher in females in September in both 2014 (♀ 135.44 ± 6.76, ♂ 109.66 ± 4.84, p = 0.0046) and 2015 (♀ 97.02 ± 3.07, ♂ 83.12 ± 3.36; p = 0.0066), as well as December 2014 (♀ 224.85 ± 8.81, ♂ 199.57 ± 8.59, p = 0.0350) (Figure 1d). Significant variability in the interaction between sex and soil fertilization treatment was observed for TPhC in December 2015. The highest concentration of TPhC occurred in NF males (NF♂ 206.81 ± 5.55(a)) and was significantly lower in F males (F♂ 166.47 ± 7.88 (b)). The values in both of these groups, however, were not significantly different than groups of females (NF♀ 190.90 ± 9.19 (ab), F♀ 187.58 ± 4.96 (ab), p = 0.0170) (Figure 1d, Tables S1 and S2).

Figure 1

Effect of sex (female (red) vs. male (blue)) on the concentration of (a) soluble sugars (%); (b) starch (%); (c) total nonstructural carbohydrates (TNC; %); (d) total phenolic compounds concentration (TPhC; µmol/ g−1 dry mass) in needles of Juniperus communis measured at eight time points over the course of two years. Asterisks indicate significant influence of sex (p < 0.05). Values for time points with a significant interaction between sex and soil fertilization treatment (fertilized F vs. nonfertilized NF) are illustrated in the inset.

Female individuals exhibited a higher concentration of C than males (Figure 2a) in the colder months, i.e., in September 2014 (♀ 48.97 ± 0.13, ♂ 48.36± 0.14, p = 0.0027) and 2015 (♀ 49.25 ± 0.17, ♂ 48.71 ± 0.15, p = 0.0251), as well as in December 2014 (♀ 49.17 ± 0.09, ♂ 48.75 ± 0.12, p = 0.0112) and 2015 (♀ 49.41 ± 0.13, ♂ 49.05 ± 0.11, p = 0.0407) (Figure 2a). In contrast, males had a higher concentration of n class="Chemical">N iclass="Chemical">n March 2014 (♀ 1.73± 0.17, ♂ 2.00 ± 0.16, p = 0.0560) aclass="Chemical">nd Juclass="Chemical">ne 2014 (♀ 1.32 ± 0.14, ♂ 1.54 ± 0.10, p = 0.0140) (Figure 2b). class="Chemical">n class="Chemical">Notably, a significant interaction between sex and soil fertilization treatment was observed for N concentration in June 2014, where F plants of both sexes had the highest N concentration (F♀ 1.75 ± 0.08(a) and F♂ 1.77 ± 0.06(a)), and whose values were significantly different from NF plants. The lowest concentration of N, however, was observed in NF females (NF♀ 0.91 ± 0.06(c) and NF♂ 1.23 ± 0.11(b), p = 0.0242) (Figure 2b, Tables S1 and S2).

Figure 2

Effect of sex (female (red) vs. male (blue)) on the concentration of (a) carbon (%); (b) nitrogen (%); (c) C:N ratio in needles of Juniperus communis measured at eight time points over the course of two years. Asterisks indicate significant influence of sex (p < 0.05). Values for time points with a significant interaction between sex and soil fertilization treatment (fertilized F vs. nonfertilized NF) are illustrated in the insets.

The C:n class="Chemical">N ratio was higher iclass="Chemical">n males iclass="Chemical">n March 2015 (♀ 28.73 ± 2.91, ♂ 34.70 ± 4.09, p = 0.0152), aclass="Chemical">nd higher iclass="Chemical">n females iclass="Chemical">n Juclass="Chemical">ne 2014 (♀ 38.38 ± 4.00, ♂ 31.52 ± 1.98, p = 0.0016) aclass="Chemical">nd September 2015 (♀ 29.44 ± 2.08, ♂ 26.12 ± 2.11, p = 0.0234) (Figure 2c). The C:class="Chemical">n class="Chemical">N ratio exhibited significant differences in regards to the interaction between sexes and soil fertilization treatment in June 2014 and in March 2015. Both sexes had the lowest C:N ratio in F plants in June 2014 (F♀ 27.13 ± 1.45(c), F♂ 27.12 ± 0.95(c)), when NF females had the highest C:N ratio (NF♀ 53.56 ± 3.32(a)), a value that was significantly greater than NF males (NF♂ 39.85 ± 3.76(b), p = 0.0027) (Tables S1 and S2). Fertilized plants still had the lowest C:N ratio regardless of sex in March 2015, however, the highest values were observed in NF males and intermediate values in NF females (C:N ratio: NF♀ 41.53 ± 3.69(b), F♀ 20.78 ± 0.58(c), NF♂ 44.62 ± 1.61(a), F♂ 24.80 ± 3.80(c), p = 0.0207) (Figure 2c, Tables S1 and S2).

n class="Chemical">Phosphorus coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n was higher iclass="Chemical">n male thaclass="Chemical">n iclass="Chemical">n female iclass="Chemical">ndividuals duriclass="Chemical">ng the first four sampled time poiclass="Chemical">nts (March 2014 ♀ 0.19 ± 0.01, ♂ 0.24 ± 0.01, p = 0.0041; July 2014 ♀ 0.14 ± 0.01, ♂ 0.16 ± 0.01, p = 0.0186; September 2014 ♀ 0.18 ± 0.01, ♂ 0.20 ± 0.01, p = 0.0255; December 2014 ♀ 0.18 ± 0.01, ♂ 0.24 ± 0.01, p = 0.0003) aclass="Chemical">nd was theclass="Chemical">n reversed (higher iclass="Chemical">n females) at the fifth time poiclass="Chemical">nt (March 2015 ♀ 0.23 ± 0.02, ♂ 0.19 ± 0.02, p = 0.0483) (Figure 3a). A sigclass="Chemical">nificaclass="Chemical">nt iclass="Chemical">nteractioclass="Chemical">n betweeclass="Chemical">n sex aclass="Chemical">nd soil fertilizatioclass="Chemical">n treatmeclass="Chemical">nt was observed for P levels iclass="Chemical">n September 2015. At that time, fertilized females had a sigclass="Chemical">nificaclass="Chemical">ntly lower coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n of P thaclass="Chemical">n aclass="Chemical">ny of the other sample groups (class="Chemical">n class="Chemical">phosphorus: NF♀ 0.23 ± 0.01 (a), F♀ 0.18 ± 0.00 (b), NF♂ 0.22 ± 0.02 (a), F♂ 0.22 ± 0.01 (a), p = 0.0330) (Figure 3a, Tables S1 and S2).

Figure 3

Effect of sex (female (red) vs. male (blue)) on the concentration of (a) phosphorus (%); (b) potassium (%); (c) calcium (%); (d) magnesium (%) in needles of Juniperus communis measured at eight time points over two years. Asterisks indicate significant influence of sex (p < 0.05). Values for time points with a significant interaction between sex and soil fertilization treatment (fertilized, F vs. nonfertilized, NF) are illustrated in the insets.

The concentration of K was higher in male individuals during periods corresponding to the accumulation of biomass in roots, during both years (September 2014 ♀ 0.80 ± 0.01, ♂ 0.88 ± 0.04, p = 0.0080; September 2015 ♀ 0.62 ± 0.02, ♂ 0.69 ± 0.02, p = 0.0238) (Table S2). K levels were also higher in males in July 2014 (♀ 0.72 ± 0.02, ♂ 0.84 ± 0.02, p = 0.0002) and December 2014 (♀ 0.58 ± 0.03, ♂ 0.66 ± 0.02, p = 0.0126) (Figure 3b). A significant interaction between sex and soil fertilization treatment was observed in September 2014, when fertilized males had a significantly higher concentration of K than any of the other sample groups (n class="Chemical">potassium: class="Chemical">n class="Chemical">NF♀ 0.80 ± 0.03 (b), F♀ 0.81 ± 0.01 (b), NF♂ 0.80 ± 0.03 (b), F♂ 0.99 ± 0.04 (a), p = 0.0091) (Figure 3b, Tables S1 and S2).

The concentration of n class="Chemical">calcium was higher iclass="Chemical">n female iclass="Chemical">ndividuals prior to the period of strobili opeclass="Chemical">niclass="Chemical">ng iclass="Chemical">n March 2014 (♀ 1.04 ± 0.07, ♂ 0.86 ± 0.04, p = 0.0001) aclass="Chemical">nd theclass="Chemical">n reversed with higher levels of class="Chemical">n class="Chemical">calcium in males prior to strobili opening in March 2015 (♀ 0.89 ± 0.09, ♂ 1.01 ± 0.09, p = 0.0455) and during the dormant period (December 2014 ♀ 0.98 ± 0.09, ♂ 1.20 ± 0.11, p = 0.0231) (Figure 3c). A significant interaction between soil fertilization treatment and sex in the level of Ca was observed in July 2014, where NF males had a significantly higher level of Ca than any of the other sample groups (calcium: NF♀ 0.83 ± 0.05 (b), F♀ 0.71 ± 0.06 (b), NF♂ 1.09 ± 0.04 (a), F♂ 0.65 ± 0.02 (b), p = 0.0043) (Figure 3c, Tables S1 and S2).

The concentration of n class="Chemical">magnesium was higher iclass="Chemical">n males iclass="Chemical">n July 2014 (♀ 0.11 ± 0.00, ♂ 0.13 ± 0.00, p = 0.0004) aclass="Chemical">nd September 2014 (♀ 0.16 ± 0.01, ♂ 0.22 ± 0.01, p < 0.0001) (Figure 3d, Table S2).

3. Discussion

The different functional roles of males and females in dioecious species may result in different strategies of resource allocation (e.g., [37,38]). Results of our present study confirmed that females allocate more resources to storage and defense than males, thus males exhibited a lower accumulation of n class="Chemical">starch, Tclass="Chemical">n class="Chemical">NC, carbon, and TPhC than females in their needles (leaves). Those concentrations of carbohydrates and C, however, were not related to availability of nutrients as initially hypothesized. Our results on carbohydrates did confirm, as we hypothesized, that differences between both sexes generally appear during the time directly after strobili opening (in June) when the most intensive period of vegetative growth occurs in both sexes of plants, but females are also allocating resources into cone development. Seed production requires significant resource demands which can result in increasing stress levels in plants [39], and the increase of accumulation of nonstructural carbohydrates is directly associated with plant response to stress conditions [40]. In our study, however, no significant interaction between sex and soil fertilization treatment was observed for carbohydrate accumulation, so the differences in carbohydrate levels reflect an allocation strategy rather than a stress response.

3.1. Carbohydrates

In our study, low nutrient availability resulted in increased accumulation of Tn class="Chemical">NC aclass="Chemical">nd class="Chemical">n class="Chemical">starch in needles of both sexes. These findings are similar to the results obtained in Salix paraplesia where low nutrient availability resulted in an increase in the accumulation of nonstructural carbohydrates and, similar to our findings, the concentration was higher in females and no interaction between sex and nutrient availability was observed for carbohydrate levels [38]. Lack of interaction between sex and soil fertilization in our studies result in rejection of our second hypothesis. Different studies show that female plants had higher levels of soluble sugars than male plants in leaves of Populus cathayana when they were grown under elevated CO2 and N deposition [14]. Similarly, female plants of Tinospora cordifolia had higher concentrations of total sugars and starch than male plants in different seasons [15]. Additional comparative studies with a greater number of species need to be conducted to definitely determine if a greater accumulation of carbohydrates in females of dioecious perennial plants is a general rule. Studies in species that exhibit sex plasticity, however, have demonstrated that plants which produce and maintain female generative organs have higher long-term concentrations of TNC [41]. We assume that the greater accumulation of carbohydrates in female plants of J. communis is related to the different reproductive roles of male and female plants rather than to a stress response. This conclusion is supported by a similar carbohydrate accumulation response in both sexes when they were grown under different levels of soil fertilization, as well as the lack of a statistically significant interaction between sex and soil fertilization treatment.

The accumulation of soluble n class="Chemical">sugars is part of a short-time respoclass="Chemical">nse to class="Chemical">n class="Disease">stress conditions [28]. Nutrient availability did not impact the concentrations of soluble sugars, indicating that their synthesis does not play a role in the response of J. communis to different levels of soil fertilization, but rather that differences in soluble sugars observed between male and female individuals in July 2015 are connected to the different energy demands required by female plants for cone development. This observation stands in contrast to a study on Populus cathayana, where greater N deposition in the environment resulted in a greater shift from starch to soluble sugars in females than in males, while excessive accumulation of starch and soluble sugars was observed in females under control conditions [42].

Male individuals can adopt an energy-saving strategy when they grow in nutrient deficient conditions [43]. In our current study, however, a significant interaction between soil fertilization treatment and sex in the concentration of n class="Chemical">carbohydrates was class="Chemical">not observed, coclass="Chemical">ntrary to our secoclass="Chemical">nd hypothesis. Iclass="Chemical">nstead, our results iclass="Chemical">ndicated that females had a higher accumulatioclass="Chemical">n of class="Chemical">n class="Chemical">carbohydrates than male plants; independent of nutrient availability.

n class="Chemical">Starch represeclass="Chemical">nts aclass="Chemical">n eclass="Chemical">nergy reserve, therefore, its coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n is geclass="Chemical">nerally low duriclass="Chemical">ng active periods of placlass="Chemical">nt growth aclass="Chemical">nd high duriclass="Chemical">ng the wiclass="Chemical">nter wheclass="Chemical">n placlass="Chemical">nts are dormaclass="Chemical">nt [15,44]. Iclass="Chemical">n coclass="Chemical">ntrast, the coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n of total soluble class="Chemical">n class="Chemical">sugars is generally the highest in summer [15], but not always [45]. In our study, the lowest concentrations of starch were observed in September and December in both years. This phenomenon is observed in coniferous, evergreen species, which in contrast to deciduous trees, maintain their metabolism during winter and in colder months decrease in starch [46,47] and increase in fat was observed [47]. In addition, differences in starch levels were evident between male and female plants in June. Notably, the highest air temperatures also occurred at this time, which may have affected starch accumulation as elevated temperature (more than 25 °C) can inhibit starch synthesis [48]. Additionally, our previous study shows that the high temperatures can also have a differential impact on leaf mass area (LMA) values in male and female plants; with females exhibiting a significantly higher LMA during the hottest month of the two years of the study (females 156.78 ± 3.13 and males 147.35 ± 3.23, p = 0.0465) [32]. The thicker lamina present in leaves of female plants, relative to male plants, can better protect cells against a sharp rise in temperature [49]. As a consequence of this protective feature, starch synthesis can be maintained in female plants.

In studies conducted during the same time on male and female plants of n class="Species">J. communis it was fouclass="Chemical">nd that the two sexes accumulate a similar amouclass="Chemical">nt of total biomass regardless of class="Chemical">nutrieclass="Chemical">nt availability, however, both sexes differed iclass="Chemical">n their allocatioclass="Chemical">n to abovegrouclass="Chemical">nd vs. root tissues [50], data class="Chemical">not published]. Therefore, it is plausible that the differeclass="Chemical">nt strategies of class="Chemical">n class="Chemical">carbohydrate allocation (greater TNC, starch, and soluble sugars in females than in males) do not influence plant growth and are probably associated to a greater extent with differences in their allocation to generative functions and defense.

3.2. Carbon, Nitrogen, C:N Ratio, and Other Elements

Increased n class="Chemical">nitrogen depositioclass="Chemical">n has beeclass="Chemical">n previously observed to iclass="Chemical">nduce aclass="Chemical">n iclass="Chemical">ncrease iclass="Chemical">n the level of class="Chemical">n class="Chemical">carbon in female individuals of Populus cathayana, where the concentration of C was lower in control females than those which received nitrogen supplementation [42]. In that study, however, carbon concentration was independent of nitrogen deposition in males [42]. In our current study, C concentration was higher in females than males, independent of fertilizer treatment, although carbon levels were higher in F plants than in NF plants. These data indicate that despite an increase in C accumulation in fertilized plants, females have mechanisms that allow them to maintain a higher concentration of C, relative to males, even in a nutrient deficient environment.

A higher n class="Chemical">N coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n was observed iclass="Chemical">n class="Chemical">needles of class="Chemical">n class="Species">J. communis male plants in March and June 2014 and a higher C:N ratio was observed in March 2015. These data can suggest that males require more N than females in order to produce pollen [51], a requirement that could have a significant impact when plants are grown in a low nutrient environment (see Figure 2b,c). As an extension, it is plausible to suggest that nonfertilized males allocate their limited N supply to strobili rather than leaves. Differences in the C:N ratio can be the result of differences in the allocation of these elements between the sexes. Under conditions of elevated CO2 and N deposition, a higher C:N ratio in Populus cathayana was observed in roots of male individuals whereas the concentration of N in leaves was also greater in males than in females [14]. Consistent with our second hypothesis, a greater C:N ratio was observed in nonfertilized female individuals, relative to nonfertilized males, in June 2014. These data indicate that females are more depleted of nitrogen than males under nutrient-limited conditions, where the nitrogen is probably allocated to seed production. On the other hand, the higher C:N ratio observed in females was not always influenced by nutrient availability, thus providing additional evidence for sex differences in nutrient allocation strategies.

Males require n class="Chemical">nitrogen to produce polleclass="Chemical">n aclass="Chemical">nd male iclass="Chemical">nfloresceclass="Chemical">nces have a greater coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n of class="Chemical">n class="Chemical">nitrogen than female inflorescences [4]. Notably, a lower level of nitrogen was detected in females of Mercurialis perennis during the reproductive stage, which was attributed by the authors to nitrogen allocation to seeds [52]. Similar conclusions have also been formulated by Bañuelos and Obeso [8]. Despite the previous demonstration of a greater allocation of N to male cones [13], females allocate more N and P to reproduction than males where those elements are mainly used in seed and fruit production [4]. In accordance to our first hypothesis, this strategy is most likely present in J. communis where females exhibit lower concentrations than males in N, as well as the other measured macroelements. Moreover, the concentration of K in female generative structures has been reported to increase during the transition from flowers to seeds and accompanying structures [4]. Notably, differences between male and female plants in the concentration of K in our present study appeared after strobili opening (a process analogous to flowering). This confirms our third hypothesis and indicates that K may be allocated more intensively to generative organs in females during seed formation. Differences in K allocation were also confirmed by results obtained in F and NF plants where fluctuations in the differences between the soil fertilization treatments were observed, indicating that fertilized plants allocate or use K differently, for example, to increase growth.

Low concentrations of n class="Chemical">nitrogen caclass="Chemical">n iclass="Chemical">nflueclass="Chemical">nce growth iclass="Chemical">n female placlass="Chemical">nts due to lower photosyclass="Chemical">nthetic capacity [23]. Iclass="Chemical">n the curreclass="Chemical">nt study, the lower levels of class="Chemical">n class="Chemical">nitrogen detected in needles of female plants of J. communis may be connected to the lower photosynthetic efficiency of females detected in a previous study [32]. It has been previously suggested that male individuals of J. thurifera use available nutrients to increase gas exchange capacity, while females exhibit a long-term strategy and increase N storage, saving N-reserves for reproduction [53]. In this regard, female plants of Populus cathayana have been reported to exhibit a higher level of plasticity in N allocation than male plants in response to increased N deposition and use N more efficiently in response to N enrichment [42].

Fertilized females probably use any additional P and K to increase reproduction while fertilized males store excess elements in leaves. n class="Chemical">Noclass="Chemical">nfertilized females have class="Chemical">no reduclass="Chemical">ndaclass="Chemical">nt resources aclass="Chemical">nd thus have similar coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">ns of macroelemeclass="Chemical">nts as class="Chemical">noclass="Chemical">nfertilized males. This suggestioclass="Chemical">n, however, is coclass="Chemical">ntrary to our secoclass="Chemical">nd hypothesis; as well as the ficlass="Chemical">ndiclass="Chemical">ngs of Xia et al. [22] where female placlass="Chemical">nts growiclass="Chemical">ng uclass="Chemical">nder high P supply exhibited a greater coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n of P iclass="Chemical">n leaves thaclass="Chemical">n male placlass="Chemical">nts.

3.3. Phenolic Compounds

Female and male individuals can differ in their response to biotic and n class="Disease">abiotic stresses. Oclass="Chemical">ne type of class="Chemical">n class="Disease">stress response is to increase the concentration of total phenolic compounds (TPhC). In dioecious species, female plants generally accumulate a higher level of TPhC than male plants [3,15,54]. The increased accumulation occurs regardless of habitat [55] and in different seasons [15], however, this is not always the case [52]. Our results are similar to the majority of studies and are in agreement with our first hypothesis. Female plants exhibited a higher concentration of TPhC, and in accordance to our third hypothesis, differences between sexes were the most pronounced in autumn and winter.

Female and male plants can respond differently to n class="Disease">stress coclass="Chemical">nditioclass="Chemical">ns iclass="Chemical">n regard to class="Chemical">n class="Chemical">TPhC. Drought stress can decrease TPhC in females but not in males [6]. Warmer temperatures can decrease TPhC levels while UV-B radiation can increase the concentration of phenolic glycosides in leaves [54]. Increased levels of UV-B also induce an increase in some phenolic compounds in female plants of Populus tremula, but not in males [54]. Moreover, increased temperature and UV increased the concentration of secondary metabolites in female plants of Salix myrsinifolia [16].

Warm temperatures and elevated n class="Chemical">CO2 caclass="Chemical">n sometimes iclass="Chemical">nduce similar iclass="Chemical">ncreases iclass="Chemical">n growth aclass="Chemical">nd the accumulatioclass="Chemical">n of class="Chemical">n class="Chemical">phenolic compounds in both male and female plants [56]. In our present study, however, during the period of the highest temperatures TPhC concentrations were relatively low and did not differ between sexes. On the other hand, fruiting has been demonstrated to reduce the concentration of secondary metabolites [3]. However, it is important to note that this was not observed in our study, where female plants of J. communis were found to have a higher level of TPhC than male plants, which do not bear cones.

In our study, n class="Chemical">NF placlass="Chemical">nts exhibited a higher coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">n of class="Chemical">n class="Chemical">TPhC than F plants, indicating that TPhC in J. communis is produced as a response to stress associated with nutrient limitation. On the other hand, carbon concentration is higher in F plants while carbohydrates and TPhC concentrations are higher in NF plants, which may indicate that F plants invest carbon differently than stressed plants and probably invest more in different compounds (other than starch and phenolics) than NF plants or have different allocation strategy. It was shown previously that phosphorus availability influence allocation of resources and P-limited plants invested more in constitutive and induced chemical defenses while fertilized plants invested more in growth [57].

A recent study of Song et al. [25] reported that female individuals of n class="Species">Populus cathayana exhibited a higher level of secoclass="Chemical">ndary metabolite activity thaclass="Chemical">n males iclass="Chemical">n respoclass="Chemical">nse to class="Chemical">n class="Chemical">nitrogen deficiency. Males plants, however, had greater stress tolerance, and did not invest as much in defense as females [25]. A similar response was present in our study where male plants had a lower concentration of TPhC than female plants during the winter months when cold stress was severe.

We found that male and female plants can sometimes (December) respond differently to nutrient limitation and that a higher concentration of n class="Chemical">TPhC was preseclass="Chemical">nt iclass="Chemical">n class="Chemical">n class="Chemical">NF males compared to F males. However, no differences were apparent between females growing under different soil fertilization treatments. These finding are in contrast to the study of Randriamanana et al. [58] where no differences between male and female plants of Populus tremula were found in regards to the concentration of phenolics in plants grown in different levels of N and P fertilization. Our findings indicate that in J. communis, higher levels of phenolic compounds in female plants can be independent of nutrient conditions, while in males, differences can be connected to the nutrient stress response. We assume that the main function of phenolics in J. communis is to protect plant from the adverse impact of low temperatures because of the observed elevation of TPhC levels in autumn and winter [59].

4. Materials and Methods

4.1. Plant Material

Fifty shoots of n class="Species">Juniperus communis L. were collected iclass="Chemical">n 2012 from each of 10 male aclass="Chemical">nd 10 female mature placlass="Chemical">nts growiclass="Chemical">ng iclass="Chemical">n the Rokita forest district, Westerclass="Chemical">n Pomeraclass="Chemical">nia, Polaclass="Chemical">nd. Shoots were takeclass="Chemical">n from the middle part of braclass="Chemical">nches aclass="Chemical">nd were choseclass="Chemical">n from braclass="Chemical">nches that had class="Chemical">no evideclass="Chemical">nce of reproductive developmeclass="Chemical">nt. A total of 1000 shoots were rooted, traclass="Chemical">nsferred to the Iclass="Chemical">nstitute of Declass="Chemical">ndrology, Polish Academy of Scieclass="Chemical">nces iclass="Chemical">n Kórclass="Chemical">nik, Polaclass="Chemical">nd aclass="Chemical">nd maiclass="Chemical">ntaiclass="Chemical">ned iclass="Chemical">n a greeclass="Chemical">nhouse iclass="Chemical">n 10-litre pots uclass="Chemical">nder two-meter-high scaffoldiclass="Chemical">ng covered with a shadiclass="Chemical">ng class="Chemical">net. The shadiclass="Chemical">ng class="Chemical">net reduced full suclass="Chemical">nlight by 50% aclass="Chemical">nd it was coclass="Chemical">nfirmed by the measuremeclass="Chemical">nts of the relative photosyclass="Chemical">nthetic photoclass="Chemical">n flux declass="Chemical">nsity iclass="Chemical">nside the shaded caclass="Chemical">nopy. For this purpose a liclass="Chemical">ne quaclass="Chemical">ntum seclass="Chemical">nsor (Apogee Iclass="Chemical">nc., Logaclass="Chemical">n, UT, USA) was used accordiclass="Chemical">ng to the method of Messier aclass="Chemical">nd Puttoclass="Chemical">neclass="Chemical">n [60]. The soil substrate used iclass="Chemical">n the experimeclass="Chemical">nt was takeclass="Chemical">n from a mixed broadleaved forest. A total of 10% of the soil volume origiclass="Chemical">nated from the locatioclass="Chemical">n iclass="Chemical">n which the materclass="Chemical">nal placlass="Chemical">nts grew to provide the iclass="Chemical">ntroductioclass="Chemical">n of mycorrhizae. Pots were class="Chemical">n class="Chemical">watered separately by an automatic irrigation system. Plants from each group of soil fertilization treatment were irrigated with different amounts of water as soil fertilization increases the growth of the plant and water demands. To provide plants with sufficient amount of water and avoid water stress fertilized individuals got twice the volume of water as nonfertilized plants. Plants were watered each day to keep the medium soil moisture during the whole vegetation season.

4.2. Experimental Design and Sampling

Potted seedlings of both sexes were randomly divided into two groups in March 2013 with the same number of male (♂) and female (♀) plants in each of the two groups. One group was provided fertilizer (F) while the other group was nonfertilized (n class="Chemical">NF). Placlass="Chemical">nts iclass="Chemical">n the F group were fertilized every year iclass="Chemical">n May, after strobili opeclass="Chemical">niclass="Chemical">ng had occurred. The fertilizer applicatioclass="Chemical">n coclass="Chemical">nsisted of five grams of slow-release fertilizer (15.0% class="Chemical">n class="Chemical">N, 9.0% P, 12.0% K, 2.5% MgO and microelements; Osmocote Exact, ICL, Israel) per liter. Plants in the NF group were grown without the addition of any supplemented fertilizer. Each treatment group (F and NF) contained the same number of plants with the same paternal or maternal origin. Plants were further divided into two blocks containing the same number of male and female plants from each treatment group.

Whole plants were harvested four times per year (March, June, September, and December) in 2014 and 2015. Sampling dates were linked to plant n class="Chemical">phenology, with the first (March) occurriclass="Chemical">ng before strobili opeclass="Chemical">niclass="Chemical">ng; the secoclass="Chemical">nd (Juclass="Chemical">ne) occurriclass="Chemical">ng immediately after polliclass="Chemical">natioclass="Chemical">n, duriclass="Chemical">ng the most iclass="Chemical">nteclass="Chemical">nsive period of vegetative growth; the third (September/October) occurriclass="Chemical">ng duriclass="Chemical">ng biomass allocatioclass="Chemical">n primarily to roots; the fourth (December) occurriclass="Chemical">ng wheclass="Chemical">n placlass="Chemical">nts were dormaclass="Chemical">nt. This patterclass="Chemical">n of sampliclass="Chemical">ng was repeated iclass="Chemical">n both years. Placlass="Chemical">nts did class="Chemical">not exhibit preseclass="Chemical">nce of strobili iclass="Chemical">n the first year of collectioclass="Chemical">n (2014) but all of the harvested placlass="Chemical">nts exhibited coclass="Chemical">ne iclass="Chemical">nitiatioclass="Chemical">n aclass="Chemical">nd strobili opeclass="Chemical">niclass="Chemical">ng iclass="Chemical">n the secoclass="Chemical">nd year (2015). A total of 24 iclass="Chemical">ndividuals were harvested oclass="Chemical">n each sampliclass="Chemical">ng date. The same class="Chemical">number of fertilized aclass="Chemical">nd class="Chemical">noclass="Chemical">nfertilized, female aclass="Chemical">nd male placlass="Chemical">nts were harvested each time. Three iclass="Chemical">ndividuals from each pareclass="Chemical">ntal origiclass="Chemical">n were desigclass="Chemical">nated for collectioclass="Chemical">n at each sampliclass="Chemical">ng time (3 iclass="Chemical">ndividuals × 2 sexes × 2 treatmeclass="Chemical">nts × 2 blocks = 24).

Whole plants were harvested and dried at 65 °C for 72 h. All needles were separated from their respective shoots and used in downstream analyses. n class="Chemical">Needles were grouclass="Chemical">nd to a ficlass="Chemical">ne powder prior to use with the aid of a Mikro-Feiclass="Chemical">nmühle Culatti mill (IKA Labortechclass="Chemical">nik, Staufeclass="Chemical">n, Germaclass="Chemical">ny).

4.3. Microclimate

Four EL-USB-2+ data loggers (Lascar electronics, Wiltshire, United Kingdom) were placed near the top of plants to monitor temperature and humidity. Meteorological parameters were measured every hour over the entire two-year duration of the study. Monthly mean, minimal, and maximal temperatures were calculated, as well as monthly mean relative humidity values, for the entire two-year sampling period (Table 1). Monthly mean temperatures ranged between 18.14 ± 7.95 °C (mean ± SE) in June 2014 and −2.00 ± 5.97 °C in December 2015. The highest and lowest temperatures occurred in September and December 2015, respectively. The difference between the highest and lowest monthly temperature throughout the entire study was 60 °C. The lowest relative humidity occurred in June 2014 (RHmin= 69.70 ± 22.49) and the highest in December 2014 (RHmax = 92.21 ± 8.37). The difference between the lowest and the highest mean relative humidity throughout the study was 22.51%.

Table 1

Air temperature (mean monthly, minimum, and maximum monthly temperatures) and mean monthly relative humidity monitored near the top of J. communis plants throughout the two-year study. Data were registered hourly and are presented as a mean ± SE (n = 745).

Dates		Monthly Temperature (°C)			Relative Air Humidity (%)
		Mean	Minimum	Maximum
I year	March 2014	7.47 ± 7.97	−6.0	29.5	76.51 ± 21.43
	June 2014	18.14 ± 7.95	4.0	40.5	69.70 ± 22.49
	September 2014	16.49 ± 7.32	−1.0	36.0	78.82 ± 20.82
	December 2014	1.58 ± 3.33	−8.0	14.5	92.21 ± 8.37
II year	March 2015	5.68 ± 6.53	−8.5	26.0	76.99 ± 20.54
	June 2015	17.89 ± 7.95	4.0	43.0	72.34 ± 23.32
	September 2015	15.10 ± 7.31	0.5	44.5	76.31 ± 19.13
	December 2015	−2.00 ± 5.97	−15.5	13.5	91.37 ± 8.85

4.4. Sugars and Starch

A modification of the methods described by Haissig and Dickson [61], as well as Hansen and Møller [62], were used to determine the concentration of total nonstructural n class="Chemical">carbohydrates (Tclass="Chemical">n class="Chemical">NC) and starch, as previously described in Oleksyn et al. [63]. Carbohydrates were extracted from powdered needle tissue in a 12:5:3 ratio solution of methanol:chloroform:water. The concentration of soluble sugars in the extracts was determined colorimetrically using anthrone reagent and measured at 625 nm, while starch concentration was determined in tissue residue by enzymatically converting the starch to glucose with amyloglucosidase. Absorbance was measured at 450 nm after a 30 min incubation at 25 °C in peroxidase-glucose oxidase-odianisidine dihydrochloride. The concentrations of soluble sugars and starch are expressed as a percentage of glucose per g of needle dry mass. Glucose standards were used to generate a standard curve using linear regression and the standard curve was used to estimate the concentration of soluble sugars in the samples.

4.5. Elemental Analysis

The concentration of a variety of elements was determined in powdered, leaf-tissue samples. The percentage of n class="Chemical">nitrogen aclass="Chemical">nd class="Chemical">n class="Chemical">carbon in samples was determined using an Elemental Combustion System CHNS/O Analyser 2400 Series II (Perkin Elmer; Costech Analytical Technologies Inc., Valencia, CA, USA). The percentage of phosphorus, potassium, calcium, and magnesium in the samples was determined using an inductively coupled plasma–optical emission spectroscope (ICP–OES). Prior analysis samples were mineralized in nitric acid. Calibration was conducted by external standard method according to the standard PN-EN ISO 11885:2009 [64].

4.6. Total Phenols

A total of 0.1 g of tissue powder was used to determine the concentration of total n class="Chemical">phenols. Samples were boiled for 15 miclass="Chemical">n iclass="Chemical">n 95% class="Chemical">n class="Chemical">ethanol and 10 min in 80% ethanol. Folin–Ciocalteu Phenol Reagent (Sigma F-9252) was used and the concentration of total phenols was determined spectrophotometrically by measuring absorbance at 660 nm as described by Johnson and Schaal [65] and modified by Singleton and Rossi [66]. The concentration of total phenolic compounds (TPhC) is expressed as µmol of chlorogenic acid per g−1 dry mass.

4.7. Statistical Analysis

Data were analyzed using JMP® 15.1 Pro software (SAS Institute Inc., Cary, n class="Chemical">NC, USA, 1989–2019). Prior statistical aclass="Chemical">nalysis of results obtaiclass="Chemical">ned as perceclass="Chemical">ntages were calculated with Bliss correctioclass="Chemical">n [67]. Outliers were ideclass="Chemical">ntified usiclass="Chemical">ng methods as described by Deaclass="Chemical">n et al. [68] aclass="Chemical">nd studeclass="Chemical">ntized residual plots aclass="Chemical">nd residual class="Chemical">normal quaclass="Chemical">ntile plots were aclass="Chemical">nalyzed. Homogeclass="Chemical">neity of variaclass="Chemical">nces was checked usiclass="Chemical">ng Leveclass="Chemical">ne’s test aclass="Chemical">nd all data were tested for class="Chemical">normality with the Shapiro–Wilk test. Logarythmizatioclass="Chemical">n was applied to data that did class="Chemical">not exhibit a class="Chemical">normal distributioclass="Chemical">n. Data origiclass="Chemical">naticlass="Chemical">ng from each measured term were aclass="Chemical">nalyzed usiclass="Chemical">ng a two-way Aclass="Chemical">n class="Chemical">NOVA since a different set of individuals were evaluated at each sampling term. Sex and soil fertilization treatment were used as fixed effects. The number of individual nested in a block and in the number of maternal plants were treated as random effects. A Tukey’s HSD test was used to compare mean values when variability occurred. The presented data represent the mean ± standard error (SE) and differences between means were determined to be statistically significant at p < 0.05.

5. Conclusions

In the present study, we confirmed that males and females have different strategies of resource allocation, and that females invest more resources in storage and defense relative to males. It also appeared that this allocation pattern probably represents a difference in allocation strategy rather than a difference in the response to n class="Disease">stress, because differeclass="Chemical">nt levels of soil fertilizatioclass="Chemical">n (fertilizatioclass="Chemical">n aclass="Chemical">nd class="Chemical">noclass="Chemical">nfertilizatioclass="Chemical">n) iclass="Chemical">n most cases did class="Chemical">not result iclass="Chemical">n differeclass="Chemical">nces iclass="Chemical">n sex-specific respoclass="Chemical">nses. Differeclass="Chemical">nces betweeclass="Chemical">n the sexes were class="Chemical">not evideclass="Chemical">nt over the eclass="Chemical">ntire course of the study, aclass="Chemical">nd were oclass="Chemical">nly evideclass="Chemical">nt at some sampliclass="Chemical">ng time poiclass="Chemical">nts. class="Chemical">n class="Chemical">Notably, in some cases, the higher vs. lower levels of measured compounds were completely reversed in male and female plants at different time points of the study. These data suggest that long-term studies are critical for clearly assessing differences in resource allocation and stress response in male and female plants of dioecious species. Higher concentrations of macroelements (except C) in leaves of males can indicate differences in allocation and probably reflects their deposition in developing seeds in female plants rather than in leaves. The highest values of TPhC occurred during colder months which suggests that these compounds are involved in abiotic stress response in J. communis.

Our results showed differences in sexual dimorphism within chemical composition of male and female individuals of n class="Species">J. communis. However, it might class="Chemical">not be eclass="Chemical">nough to formulate uclass="Chemical">ndeclass="Chemical">niable coclass="Chemical">nclusioclass="Chemical">ns, as we focused oclass="Chemical">nly oclass="Chemical">n the class="Chemical">needles aclass="Chemical">nd results of the aclass="Chemical">nalysis, which takes iclass="Chemical">nto accouclass="Chemical">nt also shoots aclass="Chemical">nd roots might be differeclass="Chemical">nt to some exteclass="Chemical">nt. Moreover, results were obtaiclass="Chemical">ned for rooted shoots, few years old, aclass="Chemical">nd differeclass="Chemical">nt values might be obtaiclass="Chemical">ned for mature trees, which have iclass="Chemical">nvested iclass="Chemical">n reproductioclass="Chemical">n for a loclass="Chemical">nger period.

n class="Chemical">Nevertheless, this study class="Chemical">not oclass="Chemical">nly helped to uclass="Chemical">nderstaclass="Chemical">nd the biology of class="Chemical">n class="Species">J. communis but also showed the significance of long-term analysis for proper assessing of secondary sexual dimorphism. Our results show that studies concerning ecophysiological differences between sexes should include the influence of different reproductive roles of males and females. Those roles are highly connected with seasons when specific events in reproduction and allocation of biomass occurs and it should be included in experimental design to correctly understand the biology of dioecious species.