Literature DB >> 33140748

Far-red switching DNA probes for live cell nanoscopy.

Jonas Bucevičius1, Tanja Gilat1, GraŽvydas Lukinavičius1.   

Abstract

Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5'-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.

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Year:  2020        PMID: 33140748     DOI: 10.1039/d0cc06759h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  6 in total

1.  Non-destructive monitoring of 3D cell cultures: new technologies and applications.

Authors:  Marilisa Cortesi; Emanuele Giordano
Journal:  PeerJ       Date:  2022-05-12       Impact factor: 3.061

2.  Blinking Fluorescent Probes for Tubulin Nanoscopy in Living and Fixed Cells.

Authors:  Ru Ta Gerasimaitė; Jonas Bucevičius; Kamila A Kiszka; Sebastian Schnorrenberg; Georgij Kostiuk; Tanja Koenen; Gražvydas Lukinavičius
Journal:  ACS Chem Biol       Date:  2021-11-04       Impact factor: 5.100

Review 3.  Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.

Authors:  Gabriella Gagliano; Tyler Nelson; Nahima Saliba; Sofía Vargas-Hernández; Anna-Karin Gustavsson
Journal:  Front Synaptic Neurosci       Date:  2021-11-24

4.  Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies.

Authors:  Magalie Bénard; Damien Schapman; Christophe Chamot; Fatéméh Dubois; Guénaëlle Levallet; Hitoshi Komuro; Ludovic Galas
Journal:  Int J Mol Sci       Date:  2021-10-14       Impact factor: 5.923

5.  Selective Labeling of Peptides with o-Carboranes via Manganese(I)-Catalyzed C-H Activation.

Authors:  Becky Bongsuiru Jei; Long Yang; Lutz Ackermann
Journal:  Chemistry       Date:  2022-05-23       Impact factor: 5.020

Review 6.  Opportunities for Expanding Encoded Chemical Diversification and Improving Hit Enrichment in mRNA-Displayed Peptide Libraries.

Authors:  Paddy R A Melsen; Ryoji Yoshisada; Seino A K Jongkees
Journal:  Chembiochem       Date:  2022-02-18       Impact factor: 3.461

  6 in total

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