| Literature DB >> 33140061 |
Ni Huang, Paola Perez, Takafumi Kato, Yu Mikami, Kenichi Okuda, Rodney C Gilmore, Cecilia Domínguez Conde, Billel Gasmi, Sydney Stein, Margaret Beach, Eileen Pelayo, Jose Maldonado, Bernard LaFont, Ricardo Padilla, Valerie Murrah, Robert Maile, Will Lovell, Shannon Wallet, Natalie M Bowman, Suzanne L Meinig, Matthew C Wolfgang, Saibyasachi N Choudhury, Mark Novotny, Brian D Aevermann, Richard Scheuermann, Gabrielle Cannon, Carlton Anderson, Julie Marchesan, Mandy Bush, Marcelo Freire, Adam Kimple, Daniel L Herr, Joseph Rabin, Alison Grazioli, Benjamin N French, Thomas Pranzatelli, John A Chiorini, David E Kleiner, Stefania Pittaluga, Stephen Hewitt, Peter D Burbelo, Daniel Chertow, Karen Frank, Janice Lee, Richard C Boucher, Sarah A Teichmann, Blake M Warner, Kevin M Byrd.
Abstract
Despite signs of infection, the involvement of the oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, and 15 immune cell clusters. Analysis of SARS-CoV-2 viral entry factor expression showed enrichment in epithelia including the ducts and acini of the salivary glands and the suprabasal cells of the mucosae. COVID-19 autopsy tissues confirmed in vivo SARS-CoV-2 infection in the salivary glands and mucosa. Saliva from SARS-CoV-2-infected individuals harbored epithelial cells exhibiting ACE2 expression and SARS-CoV-2 RNA. Matched nasopharyngeal and saliva samples found distinct viral shedding dynamics and viral burden in saliva correlated with COVID-19 symptoms including taste loss. Upon recovery, this cohort exhibited salivary antibodies against SARS-CoV-2 proteins. Collectively, the oral cavity represents a robust site for COVID-19 infection and implicates saliva in viral transmission.Entities:
Year: 2020 PMID： 33140061 PMCID： PMC7605572 DOI： 10.1101/2020.10.26.20219089
Source DB: PubMed Journal: medRxiv