| Literature DB >> 33139937 |
Anand Saminathan1,2, John Devany3, Aneesh Tazhe Veetil1,2, Bhavyashree Suresh1,2, Kavya Smitha Pillai1, Michael Schwake4,5, Yamuna Krishnan6,7.
Abstract
The role of membrane potential in most intracellular organelles remains unexplored because of the lack of suitable tools. Here, we describe Voltair, a fluorescent DNA nanodevice that reports the absolute membrane potential and can be targeted to organelles in live cells. Voltair consists of a voltage-sensitive fluorophore and a reference fluorophore for ratiometry, and acts as an endocytic tracer. Using Voltair, we could measure the membrane potential of different organelles in situ in live cells. Voltair can potentially guide the rational design of biocompatible electronics and enhance our understanding of how membrane potential regulates organelle biology.Mesh：
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Year: 2020 PMID： 33139937 DOI： 10.1038/s41565-020-00784-1
Source DB: PubMed Journal: Nat Nanotechnol ISSN： 1748-3387 Impact factor: 39.213