Literature DB >> 3313082

Should nagarse be used during the isolation of brain mitochondria?

E J Wilson1.   

Abstract

When nagarse is used to isolate brain mitochondria, a proportion of the nagarse stays associated with the mitochondrial fraction. This results in no detrimental affect on the respiratory activities. The nagarse is active in the presence of 2.3% sodium dodecyl sulfate and when samples are prepared for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the nagarse degrades a substantial amount of the mitochondrial proteins.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3313082     DOI： 10.1007/BF00971523

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  7 in total

1.  Mitochondrial biogenesis in cultured animal cells. I. Effect of chloramphenicol on morphology and mitochondrial respiratory enzymes.

Authors:  J H Lipton; W C McMurray
Journal:  Biochim Biophys Acta       Date:  1977-08-02

2.  Overview--preparation and properties of mitochondria from different sources.

Authors:  J Nedergaard; B Cannon
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  Preparation of synaptic and nonsynaptic mitochondria from mammalian brain.

Authors:  J C Lai; J B Clark
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

4.  A comparative study of palmitoyl-CoA synthetase activity in rat-liver, heart and gut mitochondrial and microsomal preparations.

Authors:  J W de Jong; W C Hülsmann
Journal:  Biochim Biophys Acta       Date:  1970-03-03

5.  Preferential loss of ATP-dependent long-chain fatty acid activating enzyme in mitochondria prepared using Nagarse.

Authors:  S V Pande; M C Blanchaer
Journal:  Biochim Biophys Acta       Date:  1970-02-10

6.  Respiratory activity of isolated rat brain mitochondria following in vitro exposure to oxygen radicals.

Authors:  L Hillered; L Ernster
Journal:  J Cereb Blood Flow Metab       Date:  1983-06       Impact factor: 6.200

7.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469
  7 in total
  5 in total

Review 1.  Mitochondria: isolation, structure and function.

Authors:  Martin Picard; Tanja Taivassalo; Gilles Gouspillou; Russell T Hepple
Journal:  J Physiol       Date:  2011-06-27       Impact factor: 5.182

Review 2.  Unravelling the role of epigenetics in reproductive adaptations to early-life environment.

Authors:  Ben Bar-Sadeh; Sergei Rudnizky; Lilach Pnueli; Gillian R Bentley; Reinhard Stöger; Ariel Kaplan; Philippa Melamed
Journal:  Nat Rev Endocrinol       Date:  2020-07-03       Impact factor: 43.330

3.  Lower respiratory capacity in extraocular muscle mitochondria: evidence for intrinsic differences in mitochondrial composition and function.

Authors:  Samir P Patel; Jorge L Gamboa; Colleen A McMullen; Alexander Rabchevsky; Francisco H Andrade
Journal:  Invest Ophthalmol Vis Sci       Date:  2008-09-12       Impact factor: 4.799

4.  Mitochondrial structure and function are disrupted by standard isolation methods.

Authors:  Martin Picard; Tanja Taivassalo; Darmyn Ritchie; Kathryn J Wright; Melissa M Thomas; Caroline Romestaing; Russell T Hepple
Journal:  PLoS One       Date:  2011-03-28       Impact factor: 3.240

5.  Evidence for the mitochondrial lactate oxidation complex in rat neurons: demonstration of an essential component of brain lactate shuttles.

Authors:  Takeshi Hashimoto; Rajaa Hussien; Hyung-Sook Cho; Daniela Kaufer; George A Brooks
Journal:  PLoS One       Date:  2008-08-13       Impact factor: 3.240
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.