Literature DB >> 33129

Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet.

J S Tse, Y Shibayama, B J Lin.   

Abstract

With a cytochemical method using guanylyl imidodiphosphate as a substrate, the guanylate cyclase activity was localized on the plasma membrane of A, B and D cells of islets of Langerhans isolated from the rat. Adequate control experiments were performed by a double-blind method. Parallel biochemical assay showed that guanylate cyclase activity was not completely lost after fixation with 1% glutaraldehyde and incubation with 4 mM lead nitrate. Furthermore, the depressed activity was still stimulatable with acetylcholine.

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Year:  1978        PMID： 33129     DOI： 10.1007/bf00495386

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  16 in total

1.  Cytochemical localization of adenylate cyclase and 3',5'-nucleotide phosphodiesterase in Dictyostelium.

Authors:  C J Farnham
Journal:  Exp Cell Res       Date:  1975-03-01       Impact factor: 3.905

2.  Letter: Comments on the validity of the use of lead nitrate for the cytochemical study of adenylate cyclase.

Authors:  L S Cutler
Journal:  J Histochem Cytochem       Date:  1975-10       Impact factor: 2.479

3.  Localization of adenylate cyclase in Dictyostelium discoideum. II. Cytochemical studies on whole cells and isolated plasma membrane vesicles.

Authors:  L S Cutler; E F Rossomando
Journal:  Exp Cell Res       Date:  1975-10-01       Impact factor: 3.905

4.  A rapid method for the assay of guanylate cyclase.

Authors:  G Krishna; N Krishnan
Journal:  J Cyclic Nucleotide Res       Date:  1975

5.  Separation of cyclic 3',5'-nucleoside monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl cyclase and guanyl cyclase.

Authors:  A A White; T V Zenser
Journal:  Anal Biochem       Date:  1971-06       Impact factor: 3.365

6.  Adenylylimidodiphosphate and guanylylimidodiphosphate.

Authors:  R G Yount
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

7.  Method for the isolation of intact islets of Langerhans from the rat pancreas.

Authors:  P E Lacy; M Kostianovsky
Journal:  Diabetes       Date:  1967-01       Impact factor: 9.461

Review 8.  Cyclic GMP.

Authors:  N D Goldberg; R F O'Dea; M K Haddox
Journal:  Adv Cyclic Nucleotide Res       Date:  1973

9.  Pitfalls in the use of lead nitrate for the histochemical demonstration of adenylate cyclase activity.

Authors:  A Lemay; L Jarett
Journal:  J Cell Biol       Date:  1975-04       Impact factor: 10.539

10.  Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. II. Cytochemical localization within Golgi and ER membranes.

Authors:  H Cheng; M G Farquhar
Journal:  J Cell Biol       Date:  1976-09       Impact factor: 10.539
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  3 in total

1.  Ultracytochemical localization of guanylate cyclase activity in guinea-pig peritoneal macrophages under different physiological conditions.

Authors:  G Dini; M Del Rosso
Journal:  Histochem J       Date:  1984-12

Review 2.  Histochemistry of nucleotidyl cyclases and cyclic nucleotide phosphodiesterases.

Authors:  G Poeggel; H Luppa
Journal:  Histochem J       Date:  1988-05

3.  Cytochemical demonstration of guanylate cyclase activity in cardiac muscle. Preferential localization at sarcolemma and junctional sarcoplasmic reticulum.

Authors:  W Schulze; E G Krause
Journal:  Histochemistry       Date:  1983
  3 in total

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