| Literature DB >> 3312820 |
J Sawada1, T Terao, S Itoh, M Maeda, A Tsuji, H Hosoda, T Nambara.
Abstract
Hybridoma clones producing antibodies to 17 alpha-hydroxyprogesterone (17-OHP) were established by using a 17-OHP-bovine serum albumin conjugate as an immunogen. Six representative IgG-class monoclonal antibodies of high affinity (10(8)-10(9) M-1) showed differential reactivities with several structurally related steroids. Two enzyme immunoassay (EIA) systems (fluorescence EIA and micro-EIA) for 17-OHP using OHP 4B2.2.3, which showed the lowest cross-reactivity with other steroids, were established. The micro-EIA system was shown to be applicable to the mass-screening of congenital adrenal hyperplasia.Entities:
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Year: 1987 PMID： 3312820 DOI： 10.1016/0022-4731(87)91058-2
Source DB: PubMed Journal: J Steroid Biochem ISSN： 0022-4731 Impact factor: 4.292