Yuyan Zhou1,2, Xiaoya Lu1, Li Xia1, Weiqiang Yao1, Guozheng Qin1,2, Guodong Wang1,2. 1. Drug Research and Development Center//School of Pharmacy, Wannan Medical College, Wuhu 241002, China. 2. Anhui Provincial Engineering Research Center for Polysaccharide Drugs//Anhui Provincial Key Laboratory of Active Biological Macro-molecules, Wuhu 241002, China.
Abstract
OBJECTIVE: To investigate the protective effect of arctiin with anti-inflammatory bioactivity against triptolide-induced nephrotoxicity in rats and explore the underlying mechanism. METHODS: Forty SD rats were divided into 4 groups for gastric lavage of normal saline, arctiin (500 mg/kg), triptolide (500 μg/kg), or both arctiin (500 mg/kg) and triptolide (500 μg/kg). Blood samples were collected for analysis of biochemical renal parameters, and the renal tissues were harvested for determining the kidney index and for pathological evaluation with HE staining. In the in vitro experiment, HK-2 cells were treated with arctiin and triptolide either alone or in combination, and the cell viability was determined with MTT assay; the cell morphological changes was observed using laser confocal microscopy, cell apoptosis was detected using flow cytometry, and the expressions of inflammation-related protein expression were detected by Western blotting. RESULTS: In SD rats, arctiin significantly antagonized triptolide-induced elevation of BUN, Scr and kidney index (P < 0.05) and obviously improved renal tissue damages induced by triptolide including cell swelling, vacuolization and spotty necrosis. Arctiin significantly inhibited triptolide-induced cytotoxicity in HK-2 cells and increased the cell viability at 24 h (P < 0.05). Arctiin also attenuated triptolide-induced cell morphological changes, decreased cell apoptosis rate (P < 0.05) and reversed the expressions of IκBα and nuclear p65 (P < 0.05). CONCLUSIONS: Arctiin can protect the kidney from triptolide-induced damages in rats possibly through the anti-inflammatory pathway.
OBJECTIVE: To investigate the protective effect of arctiin with anti-inflammatory bioactivity against triptolide-induced nephrotoxicity in rats and explore the underlying mechanism. METHODS: Forty SD rats were divided into 4 groups for gastric lavage of normal saline, arctiin (500 mg/kg), triptolide (500 μg/kg), or both arctiin (500 mg/kg) and triptolide (500 μg/kg). Blood samples were collected for analysis of biochemical renal parameters, and the renal tissues were harvested for determining the kidney index and for pathological evaluation with HE staining. In the in vitro experiment, HK-2 cells were treated with arctiin and triptolide either alone or in combination, and the cell viability was determined with MTT assay; the cell morphological changes was observed using laser confocal microscopy, cell apoptosis was detected using flow cytometry, and the expressions of inflammation-related protein expression were detected by Western blotting. RESULTS: In SD rats, arctiin significantly antagonized triptolide-induced elevation of BUN, Scr and kidney index (P < 0.05) and obviously improved renal tissue damages induced by triptolide including cell swelling, vacuolization and spotty necrosis. Arctiin significantly inhibited triptolide-induced cytotoxicity in HK-2 cells and increased the cell viability at 24 h (P < 0.05). Arctiin also attenuated triptolide-induced cell morphological changes, decreased cell apoptosis rate (P < 0.05) and reversed the expressions of IκBα and nuclear p65 (P < 0.05). CONCLUSIONS: Arctiin can protect the kidney from triptolide-induced damages in rats possibly through the anti-inflammatory pathway.
Authors: Jun Zhang; Li-Li Huang; Xiu-Jie Liang; Yue Wang; Na Duan; Xiao-Hong Xiang; Shuang-Shuang Shu; Ting-Ting Guo; Lei Yang; Xun Tang Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2016-06
Authors: Lei Jiang; Alexander A Shestov; Pamela Swain; Chendong Yang; Seth J Parker; Qiong A Wang; Lance S Terada; Nicholas D Adams; Michael T McCabe; Beth Pietrak; Stan Schmidt; Christian M Metallo; Brian P Dranka; Benjamin Schwartz; Ralph J DeBerardinis Journal: Nature Date: 2016-04-06 Impact factor: 49.962