Literature DB >> 3311810

Involvement of a 65 kDa phosphoprotein in the regulation of membrane fusion during exocytosis in Paramecium cells.

B Stecher1, B Höhne, U Gras, M Momayezi, R Glas-Albrecht, H Plattner.   

Abstract

Antisera were raised against a phosphoprotein of 65 kDa (PP65) from Paramecium cells (shown before to be selectively dephosphorylated during synchronous exocytosis) and specified by immunoblotting. By immunofluorescence PP65 has been localized within the cortex, beneath the cell membrane. This corresponds to data obtained by cell fractionation, applying SDS-PAGE autoradiography to cortices prepared from 32P-prelabeled cells. Antisera against PP65 inhibit exocytosis in vivo (microinjection). Applying anti-PP65 antisera in vitro to cortices we could demonstrate inhibition not only of exocytosis, but also of PP65 dephosphorylation. We conclude that PP65 is involved in the regulation of membrane fusion during exocytosis.

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Year:  1987        PMID： 3311810     DOI： 10.1016/0014-5793(87)80503-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  A cortical phosphoprotein ('PP63') sensitive to exocytosis triggering in Paramecium cells. Immunolocalization and quenched-flow correlation of time course of dephosphorylation with membrane fusion.

Authors:  B Höhne-Zell; G Knoll; U Riedel-Gras; W Hofer; H Plattner
Journal:  Biochem J       Date:  1992-09-15       Impact factor: 3.857

2.  Protein phosphatase and kinase activities possibly involved in exocytosis regulation in Paramecium tetraurelia.

Authors:  R Kissmehl; T Treptau; H W Hofer; H Plattner
Journal:  Biochem J       Date:  1996-07-01       Impact factor: 3.857

Review 3.  The role of protein kinase C and its neuronal substrates dephosphin, B-50, and MARCKS in neurotransmitter release.

Authors:  P J Robinson
Journal:  Mol Neurobiol       Date:  1991       Impact factor: 5.590

4.  Identification of isoforms of the exocytosis-sensitive phosphoprotein PP63/parafusin in Paramecium tetraurelia and demonstration of phosphoglucomutase activity.

Authors:  K Hauser; R Kissmehl; J Linder; J E Schultz; F Lottspeich; H Plattner
Journal:  Biochem J       Date:  1997-04-01       Impact factor: 3.857

Review 5.  Regulated exocytosis.

Authors:  R D Burgoyne; A Morgan
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

6.  Evidence for protein dephosphorylation as a permissive step in GTP-gamma-S-induced exocytosis from permeabilized mast cells.

Authors:  Y Churcher; K M Kramer; B D Gomperts
Journal:  Cell Regul       Date:  1990-06

7.  A 63 kDa phosphoprotein undergoing rapid dephosphorylation during exocytosis in Paramecium cells shares biochemical characteristics with phosphoglucomutase.

Authors:  T Treptau; R Kissmehl; J D Wissmann; H Plattner
Journal:  Biochem J       Date:  1995-07-15       Impact factor: 3.857

8.  In vivo analysis of the major exocytosis-sensitive phosphoprotein in Tetrahymena.

Authors:  N D Chilcoat; A P Turkewitz
Journal:  J Cell Biol       Date:  1997-12-01       Impact factor: 10.539

9.  Phosphoprotein inhibition of calcium-stimulated exocytosis in sea urchin eggs.

Authors:  T Whalley; I Crossley; M Whitaker
Journal:  J Cell Biol       Date:  1991-05       Impact factor: 10.539
  9 in total

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